| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 1,902,269:1 | +T | 100% | coding (14/342 nt) | yebY ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,902,269 | 1 | . | T | 99.0% | 326.1 / ‑4.2 | 95 | S5N (AGT→AAT) | yebY | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base T (35/59); minor base C (0/1); total (35/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 123 total aligned reads displayed.
GTGCCGCCAGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑TTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCGAGAGTCG > REL606/1902219‑1902314 | gccgccAGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑tt < 1:10504730/51‑1 (MQ=255) ccgccAGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttt < 1:7658522/51‑1 (MQ=255) ccgccAGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttt < 1:6421618/51‑1 (MQ=255) ccgccAGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttt < 1:5890439/51‑1 (MQ=255) gccAGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttttt < 1:9900017/51‑1 (MQ=255) gccAGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttttt < 1:3910452/51‑1 (MQ=255) ccAGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑tttttt < 1:7225971/51‑1 (MQ=255) ccAGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑tttttt < 1:4801937/51‑1 (MQ=255) ccAGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑tttttt > 1:1439095/1‑51 (MQ=255) cAGCGCCGCTGCAGAACTGGTGAGTAATAGAAACGCCAGAATAC‑ttttttt < 1:9096136/51‑1 (MQ=255) cAGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttttttt < 1:1292584/51‑1 (MQ=255) aGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑tttttttt < 1:2599079/51‑2 (MQ=255) aGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑tttttttt > 1:1541665/1‑50 (MQ=255) aGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑tttttttt > 1:1982241/1‑50 (MQ=255) cgccgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttttttttca < 1:10023668/51‑4 (MQ=255) cgccgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttttttttca > 1:9022863/1‑48 (MQ=255) cgccgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttttttttca < 1:8137111/51‑4 (MQ=255) cgccgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttttttttca < 1:5589972/51‑4 (MQ=255) cgccgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttttttttca < 1:4841127/51‑4 (MQ=255) gccgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttttttttcat < 1:7371765/51‑5 (MQ=255) gccgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttttttttcat < 1:3846242/51‑5 (MQ=255) ccgcTGCTGAACTGGTGAGTAACAGAAACGCCAGAATAC‑ttttttttcatc < 1:9887133/51‑6 (MQ=255) cgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTtcatca < 1:10147286/51‑1 (MQ=255) cgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTtcatca < 1:10653807/51‑1 (MQ=255) cgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTtcatca < 1:5324073/51‑1 (MQ=255) cgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTtcatca > 1:1258195/1‑51 (MQ=255) cgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTtcatca > 1:339478/1‑51 (MQ=255) cgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTtcatca < 1:2917629/51‑1 (MQ=255) cgcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTtcatca < 1:8464140/51‑1 (MQ=255) gcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTcatcat > 1:6362357/1‑51 (MQ=255) gcTGCAGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTcatcat < 1:7765391/51‑1 (MQ=255) tGCAGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCAttt > 1:7658007/1‑51 (MQ=255) tGCAGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCAttt < 1:8661122/51‑1 (MQ=255) tGCAGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCAttt > 1:5881591/1‑51 (MQ=255) aGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCg < 1:6788039/51‑1 (MQ=255) aGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCg < 1:6578346/51‑1 (MQ=255) aGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCg < 1:5814469/51‑1 (MQ=255) aGAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCg < 1:10325134/51‑1 (MQ=255) gAACTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGt < 1:6523394/51‑1 (MQ=255) aaCTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTa > 1:7990545/1‑51 (MQ=255) aaCTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTa < 1:2605834/51‑1 (MQ=255) aaCTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTa < 1:9653461/51‑1 (MQ=255) aaCTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTa > 1:1807835/1‑51 (MQ=255) cTGGTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAAt > 1:561828/1‑51 (MQ=255) ggTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATcc < 1:9552691/51‑1 (MQ=255) gTGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCa < 1:5678793/51‑1 (MQ=255) tGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCAt > 1:1181193/1‑51 (MQ=255) tGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCAt < 1:9337052/51‑1 (MQ=255) tGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCAt < 1:49642/51‑1 (MQ=255) tGAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCAt > 1:10245804/1‑51 (MQ=255) gAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATc > 1:2743537/1‑51 (MQ=255) gAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATc < 1:5538854/51‑1 (MQ=255) gAGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATc < 1:2707882/51‑1 (MQ=255) aGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCa < 1:7243921/51‑1 (MQ=255) aGTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCa < 1:3953788/51‑1 (MQ=255) gTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCaa < 1:7381486/51‑1 (MQ=255) gTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCaa < 1:7084155/51‑1 (MQ=255) gTAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCaa < 1:3868198/51‑1 (MQ=255) tAACAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCaaa < 1:4491969/51‑1 (MQ=255) aaCAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCaaaa < 1:8392796/51‑1 (MQ=255) aaCAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCaaaa < 1:7712898/51‑1 (MQ=255) aaCAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCaaaa < 1:2475240/51‑1 (MQ=255) aaCAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCaaaa < 1:6863515/51‑1 (MQ=255) aCAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCaaaaa < 1:5589371/51‑1 (MQ=255) aCAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCaaaaa < 1:1931002/51‑1 (MQ=255) aCAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCaaaaa > 1:5331628/1‑51 (MQ=255) aCAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCaaaaa < 1:7968807/51‑1 (MQ=255) cAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAg < 1:3264141/51‑1 (MQ=255) cAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAg > 1:10779274/1‑51 (MQ=255) cAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAg < 1:524764/51‑1 (MQ=255) cAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAg > 1:2603473/1‑51 (MQ=255) cAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAg > 1:9831187/1‑51 (MQ=255) cAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAg < 1:4410063/51‑1 (MQ=255) cAGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAg > 1:5859620/1‑51 (MQ=255) aGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAgg > 1:8741164/1‑51 (MQ=255) aGAAACGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAgg > 1:7637228/1‑51 (MQ=255) aaaCGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGtt < 1:9528222/51‑1 (MQ=255) aaaCGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGtt > 1:4660513/1‑51 (MQ=255) aaCGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTc > 1:2050513/1‑51 (MQ=255) aaCGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTc < 1:244600/51‑1 (MQ=255) aaCGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTc < 1:4315714/51‑1 (MQ=255) aaCGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTc > 1:1851953/1‑51 (MQ=255) aCGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCg < 1:7240776/51‑1 (MQ=255) aCGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCg > 1:5807370/1‑51 (MQ=255) cGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCga < 1:541528/51‑1 (MQ=255) cGCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCga < 1:10127143/51‑1 (MQ=255) gCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCgag > 1:5752624/1‑51 (MQ=255) gCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCgag > 1:6264312/1‑51 (MQ=255) gCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCgag > 1:5562835/1‑51 (MQ=255) gCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCgag > 1:8935918/1‑51 (MQ=255) gCCAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCgag > 1:9065371/1‑51 (MQ=255) ccAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCgaga < 1:801868/51‑1 (MQ=255) ccAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCgaga > 1:3204119/1‑51 (MQ=255) ccAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCgaga < 1:10488134/51‑1 (MQ=255) ccAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCgaga < 1:4690758/51‑1 (MQ=255) ccAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCgaga < 1:5415585/51‑1 (MQ=255) cAGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCGagag > 1:9311387/1‑51 (MQ=255) aGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCGAGAGt < 1:7565328/51‑1 (MQ=255) aGAATACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCGAGAGt > 1:1081148/1‑51 (MQ=255) aaTACTTTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCGAGAGTCg > 1:5925815/1‑51 (MQ=255) | GTGCCGCCAGCGCCGCTGCAGAACTGGTGAGTAACAGAAACGCCAGAATAC‑TTTTTTTCATCATTTTCGTAATCCATCAAAAAGGTTCGAGAGTCG > REL606/1902219‑1902314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |