| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 588,490:1 | +AA | 100% | intergenic (‑217/‑52) | ybdK ← / → insJ‑1 | gamma‑glutamyl:cysteine ligase/IS150 hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 588,490 | 1 | . | A | 100.0% | 196.5 / NA | 69 | intergenic (‑217/‑52) | ybdK/insJ‑1 | gamma‑glutamyl:cysteine ligase/IS150 hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (32/37); total (32/37) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | REL606 | 588,490 | 2 | . | A | 100.0% | 195.5 / NA | 69 | intergenic (‑217/‑52) | ybdK/insJ‑1 | gamma‑glutamyl:cysteine ligase/IS150 hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (32/37); total (32/37) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCGAAGAATTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑AAGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAAT > REL606/588440‑588531 || ggCGAAGAATTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTc > 1:8568759/1‑51 (MQ=255)ggCGAAGAATTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTc > 1:4044461/1‑51 (MQ=255)ggCGAAGAATTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTc < 1:7593767/51‑1 (MQ=255) gCGAAGAATTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑a < 1:3462012/51‑1 (MQ=255) cGAAGAATTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑aa > 1:4903700/1‑51 (MQ=255) gaagaaTTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑aaa > 1:2751171/1‑50 (MQ=255) gaagaaTTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑aaa < 1:5798168/51‑2 (MQ=255) agaaTTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑aaaag > 1:7852914/1‑48 (MQ=255) agaaTTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑aaaag < 1:458578/51‑4 (MQ=255) agaaTTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑aaaa < 1:9457220/50‑3 (MQ=255) gaaTTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑aaaagt > 1:5335688/1‑47 (MQ=255) gaaTTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑aaaag > 1:4239772/1‑47 (MQ=255) aaTTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑aaaagtt > 1:9041050/1‑46 (MQ=255) aaTTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑aaaagtt < 1:7917491/51‑6 (MQ=255) aaTTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑aaaagtt < 1:5764141/51‑6 (MQ=255) tGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACt < 1:6482558/51‑1 (MQ=38) gACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTg < 1:5593376/51‑1 (MQ=38) aCGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGc < 1:3108/51‑1 (MQ=38) aCGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGc < 1:5427084/51‑1 (MQ=38) aCGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGc > 1:946775/1‑51 (MQ=38) aCGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGc > 1:4135153/1‑51 (MQ=38) cGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCa < 1:1931148/51‑1 (MQ=38) cGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCa < 1:10609400/51‑1 (MQ=38) cGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCa > 1:8810932/1‑51 (MQ=38) cGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCa > 1:1267536/1‑51 (MQ=38) gATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCAc < 1:3056368/51‑1 (MQ=38) gATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCAc < 1:6610207/51‑1 (MQ=38) gATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCAc < 1:3203610/51‑1 (MQ=38) ttGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCAccc > 1:9954815/1‑51 (MQ=38) ttGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCAccc < 1:10870336/51‑1 (MQ=38) tGAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCa > 1:6270721/1‑51 (MQ=38) gAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCAt > 1:10406524/1‑51 (MQ=38) gAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCAt < 1:6548699/51‑1 (MQ=38) gAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCAt < 1:10640069/51‑1 (MQ=38) gAGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCAt < 1:4506448/51‑1 (MQ=38) aGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCAtt < 1:9893766/51‑1 (MQ=38) aGTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCAtt > 1:4246516/1‑51 (MQ=38) gTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCAttt < 1:9170642/51‑1 (MQ=37) gTCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCAttt < 1:3616509/51‑1 (MQ=37) tCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCAtttt > 1:3348436/1‑51 (MQ=37) tCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCAtttt < 1:4161240/51‑1 (MQ=37) tCATGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCAttt < 1:2913422/50‑1 (MQ=37) aTGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCAcc > 1:10486858/1‑43 (MQ=25) aTGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTttgt > 1:7551526/1‑51 (MQ=25) tGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTttgtt < 1:10269523/51‑1 (MQ=25) tGCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTttgtt < 1:5084315/51‑1 (MQ=25) gCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTttgttg < 1:10622870/51‑1 (MQ=25) gCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTttgttg > 1:3824507/1‑51 (MQ=25) gCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTttgttg > 1:1152440/1‑51 (MQ=25) gCAGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTttgttg < 1:7742483/51‑1 (MQ=25) aGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGa < 1:5053658/51‑1 (MQ=21) aGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGa > 1:6127233/1‑51 (MQ=21) aGAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGa < 1:2340265/51‑1 (MQ=21) gAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGAc < 1:8281706/51‑1 (MQ=21) gAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGAc > 1:9845396/1‑51 (MQ=21) gAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGAc > 1:9532317/1‑51 (MQ=21) gAAAAAAACGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGAc > 1:3295407/1‑51 (MQ=21) aaaaaaaCGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACg < 1:5490599/51‑1 (MQ=21) aaaaaaaCGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACg > 1:10938373/1‑51 (MQ=21) aaaaaaaCGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACg < 1:3814326/51‑1 (MQ=21) aaaaaaaCGGGTTCAGATTCAAAAGTTGTACTGCTCCCATTTTGTTGGACg < 1:8945973/51‑1 (MQ=18) aaaaaaCTGGTTCAGCTTCAAAAGTTGTACTGCGCCCATTTTGTTGGACGa < 1:10249759/51‑1 (MQ=18) aaaaaaCGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTttgtt > 1:9521821/1‑45 (MQ=21) aaaaaaCGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGa < 1:4143697/51‑1 (MQ=18) aaaaCGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATg > 1:5386907/1‑51 (MQ=18) aaaCGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGa < 1:507884/51‑1 (MQ=18) aaCGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGaa > 1:783393/1‑51 (MQ=17) aaCGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGaa < 1:8868692/51‑1 (MQ=17) aaCGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGaa > 1:8430086/1‑51 (MQ=17) aCGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGaaa > 1:640268/1‑51 (MQ=17) cGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAAt > 1:10687610/1‑51 (MQ=17) cGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAAt > 1:3714578/1‑51 (MQ=17) cGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAAt < 1:8220364/51‑1 (MQ=17) cGGGTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAAt > 1:120574/1‑51 (MQ=17) gggTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAATg > 1:1139627/1‑51 (MQ=14) ggTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAATgg < 1:2732124/51‑1 (MQ=14) ggTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAATgg > 1:594425/1‑51 (MQ=14) gTTCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGa > 1:3115700/1‑51 (MQ=14) ttCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGaa > 1:7034265/1‑51 (MQ=14) ttCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGaa > 1:2695409/1‑51 (MQ=14) ttCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGaa < 1:8445595/51‑1 (MQ=14) ttCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGaa > 1:4812376/1‑51 (MQ=14) tCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAAt < 1:10754860/51‑1 (MQ=11) tCAGCTTCAAAAGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAAt < 1:9846471/51‑1 (MQ=11) || GGCGAAGAATTTTGACGATTGAGTCATGCAGAAAAAAACGGGTTCAGCTTC‑‑AAGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAAT > REL606/588440‑588531 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |