BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	REL606	796,329	Δ1 bp	100%	coding (1/993 nt)	ECB_00737 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	796,329	0	T	.	100.0%	418.1 / NA	85	coding (1/993 nt)	ECB_00737	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (39/46); total (39/46)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 123 total aligned reads displayed.

AATAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCATTTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCTTCTCA  >  REL606/796279‑796375

aaTAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTccatccat                                                <  1:10733790/51‑1 (MQ=255)

aaTAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTccatccat                                                >  1:6217050/1‑51 (MQ=255)

aaTAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTccatccat                                                >  1:6827136/1‑51 (MQ=255)

aaTAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTccatccat                                                <  1:5241808/51‑1 (MQ=255)

aaTAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTccatccat                                                <  1:4361469/51‑1 (MQ=255)

 aTAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtt                                               <  1:9910701/51‑1 (MQ=255)

  tAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAttt                                              >  1:3421068/1‑51 (MQ=255)

  tAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAttt                                              <  1:5252888/51‑1 (MQ=255)

  tAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAttt                                              <  1:7574053/51‑1 (MQ=255)

  tAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAttt                                              >  1:568983/1‑51 (MQ=255)

  tAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAttt                                              >  1:5605088/1‑51 (MQ=255)

  tAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAttt                                              >  1:8986489/1‑51 (MQ=255)

   aaaCACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttt                                             <  1:3101669/51‑1 (MQ=255)

   aaaCACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttt                                             >  1:4753270/1‑51 (MQ=255)

     acacATAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttttt                                           <  1:7959575/51‑1 (MQ=255)

     acacATAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttttt                                           <  1:7730150/51‑1 (MQ=255)

      cacaTAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAttttttt                                          <  1:650515/51‑1 (MQ=255)

       acaTAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttttttc                                         >  1:8868224/1‑50 (MQ=255)

       acaTAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttttttc                                         <  1:2669874/51‑2 (MQ=255)

        caTAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttttttct                                        >  1:4272483/1‑49 (MQ=255)

        caTAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttttttct                                        >  1:1289428/1‑49 (MQ=255)

         aTAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttttttctt                                       >  1:5298357/1‑48 (MQ=255)

         aTAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttttttctt                                       <  1:2310450/51‑4 (MQ=255)

         aTAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttttttctt                                       <  1:4736799/51‑4 (MQ=255)

         aTAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttttttctt                                       <  1:7649575/51‑4 (MQ=255)

          tAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttttttcttg                                      <  1:8493339/51‑5 (MQ=255)

          tAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCAtttttttcttg                                      >  1:9445921/1‑47 (MQ=255)

           aaaaaGCAGCTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgt                                    <  1:8929864/51‑1 (MQ=255)

           aaaaaGCAGCTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgt                                    >  1:9382489/1‑51 (MQ=255)

           aaaaaGCAGCTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgt                                    >  1:8196958/1‑51 (MQ=255)

           aaaaaGCAGCTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgt                                    >  1:4378946/1‑51 (MQ=255)

            aaaaGCAGCTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgtt                                   >  1:3526100/1‑51 (MQ=255)

            aaaaGCAGCTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgtt                                   <  1:9127478/51‑1 (MQ=255)

            aaaaGCAGCTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgtt                                   <  1:821746/51‑1 (MQ=255)

            aaaaGCAGCTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgtt                                   >  1:7289103/1‑51 (MQ=255)

              aaGCAGCTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgttat                                 >  1:3541528/1‑51 (MQ=255)

               agcagcTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgttatg                                >  1:6481475/1‑51 (MQ=255)

               agcagcTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgttatg                                <  1:6420760/51‑1 (MQ=255)

                gcagcTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgttatgt                               <  1:5756437/51‑1 (MQ=255)

                 cagcTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgttatgtt                              <  1:10914885/51‑1 (MQ=255)

                 cagcTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgttatgtt                              <  1:9135001/51‑1 (MQ=255)

                 cagcTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgttatgtt                              >  1:9533956/1‑51 (MQ=255)

                 cagcTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgttatgtt                              >  1:9231622/1‑51 (MQ=255)

                  agcTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgttatgtta                             <  1:6517072/51‑1 (MQ=255)

                  agcTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgttatgtta                             >  1:3501007/1‑51 (MQ=255)

                  agcTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTtgttatgtta                             <  1:5482655/51‑1 (MQ=255)

                    cTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTAtt                           >  1:5222894/1‑51 (MQ=255)

                    cTATAAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTAtt                           >  1:10158138/1‑51 (MQ=255)

                     tataAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTg                          <  1:1959973/51‑1 (MQ=255)

                      ataAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTgg                         >  1:5758029/1‑51 (MQ=255)

                       taAATCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTg                          >  1:9939910/1‑49 (MQ=255)

                        aaaTCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTa                       <  1:470528/51‑1 (MQ=255)

                        aaaTCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTa                       >  1:5533851/1‑51 (MQ=255)

                        aaaTCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTa                       >  1:2953522/1‑51 (MQ=255)

                        aaaTCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTa                       <  1:10922272/51‑1 (MQ=255)

                         aaTCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTAc                      >  1:217756/1‑51 (MQ=255)

                          aTCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACt                     <  1:4770965/51‑1 (MQ=255)

                          aTCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACt                     <  1:9549199/51‑1 (MQ=255)

                           tCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTa                    <  1:10463767/51‑1 (MQ=255)

                           tCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTa                    <  1:4648979/51‑1 (MQ=255)

                           tCTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTa                    <  1:353245/51‑1 (MQ=255)

                            cTTAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAg                   <  1:7722548/51‑1 (MQ=255)

                             ttAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGt                  >  1:1769616/1‑51 (MQ=255)

                             ttAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGt                  >  1:10794938/1‑51 (MQ=255)

                              tAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTc                 >  1:10095383/1‑51 (MQ=255)

                              tAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTc                 <  1:2692407/51‑1 (MQ=255)

                              tAAGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTc                 <  1:5912549/51‑1 (MQ=255)

                               aaGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCa                <  1:10623647/51‑1 (MQ=255)

                               aaGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCa                >  1:8722414/1‑51 (MQ=255)

                               aaGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCa                >  1:1044202/1‑51 (MQ=255)

                                aGATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCAt               >  1:8292360/1‑51 (MQ=255)

                                 gATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCAtt              <  1:1816401/51‑1 (MQ=255)

                                 gATTGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCAtt              <  1:6556887/51‑1 (MQ=255)

                                   ttGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTg            <  1:9716583/51‑1 (MQ=255)

                                   ttGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTg            <  1:10382854/51‑1 (MQ=255)

                                    tGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGa           <  1:7609866/51‑1 (MQ=255)

                                    tGTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGa           <  1:2820831/51‑1 (MQ=255)

                                     gTTACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAg          <  1:8724403/51‑1 (MQ=255)

                                      ttACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAgg         >  1:5439074/1‑51 (MQ=255)

                                      ttACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAgg         <  1:1717473/51‑1 (MQ=255)

                                      ttACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAgg         >  1:1087627/1‑51 (MQ=255)

                                      ttACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAgg         <  1:10496877/51‑1 (MQ=255)

                                       tACTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGc        <  1:538925/51‑1 (MQ=255)

                                        aCTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCt       >  1:7330059/1‑51 (MQ=255)

                                         cTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCtt      >  1:5267905/1‑51 (MQ=255)

                                         cTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCtt      <  1:6757705/51‑1 (MQ=255)

                                         cTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCtt      >  1:8774840/1‑51 (MQ=255)

                                         cTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCtt      <  1:656402/51‑1 (MQ=255)

                                         cTCCATCCA‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCtt      <  1:2546332/51‑1 (MQ=255)

                                          tccatcca‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCTtc     >  1:647770/1‑51 (MQ=255)

                                          tccatcca‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCTtc     >  1:2398972/1‑51 (MQ=255)

                                          tccatcca‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCTtc     >  1:1503476/1‑51 (MQ=255)

                                          tccatcca‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCTtc     <  1:2867150/51‑1 (MQ=255)

                                          tccatcca‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCTtc     <  1:4600291/51‑1 (MQ=255)

                                          tccatcca‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCTtc     <  1:3449132/51‑1 (MQ=255)

                                          tccatcca‑TTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCTtc     <  1:6202262/51‑1 (MQ=255)

                                           ccatcca‑tTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCTtct    <  1:7316490/51‑1 (MQ=255)

                                            catcca‑tTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCTtctc   >  1:6485133/1‑51 (MQ=255)

                                            catcca‑tTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCTtctc   >  1:3106787/1‑51 (MQ=255)

                                             atcca‑tTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCTTCTCa  <  1:9884854/51‑1 (MQ=255)

AATAAACACATAAAAAGCAGCTATAAATCTTAAGATTGTTACTCCATCCATTTTTTTTCTTGTTATGTTATTGGTACTAGTCATTTGAGGCTTCTCA  >  REL606/796279‑796375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`