Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,384,430	C→A	S29Y (TCT→TAT)	yjeB →	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,384,430	0	C	A	100.0%	310.0 / NA	88	S29Y (TCT→TAT)	yjeB	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (45/43);  total (45/43)

CGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTCTGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAA  >  REL606/4384382‑4384477                                                 |                                                cgcTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATg                                               >  1:5414329/1‑51 (MQ=255) cgcTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATg                                               <  1:930682/51‑1 (MQ=255)  gcTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGa                                              >  1:635233/1‑51 (MQ=255)   cTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGaa                                             <  1:222897/51‑1 (MQ=255)    tGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAg                                            <  1:1425075/51‑1 (MQ=255)    tGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAg                                            <  1:2965573/51‑1 (MQ=255)    tGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAg                                            >  1:3223985/1‑51 (MQ=255)    tGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAg                                            <  1:1062371/51‑1 (MQ=255)    tGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAg                                            >  1:1689660/1‑51 (MQ=255)     gATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGt                                           >  1:4879212/1‑51 (MQ=255)      aTCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtg                                          >  1:741043/1‑51 (MQ=255)      aTCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtg                                          <  1:326546/51‑1 (MQ=255)       tCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtga                                         >  1:7260709/1‑51 (MQ=255)       tCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtga                                         <  1:6783717/51‑1 (MQ=255)       tCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtga                                         <  1:3024867/51‑1 (MQ=255)        cTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGaa                                             >  1:7017441/1‑46 (MQ=255)        cTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGaa                                             >  1:834746/1‑46 (MQ=255)         tACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGaa                                             >  1:4463471/1‑45 (MQ=255)         tACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGaa                                             >  1:1899363/1‑45 (MQ=255)         tACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtgact                                       <  1:619285/51‑1 (MQ=255)          aCATGGCGTCATTGCTAGAAGGGCGGATGACCAGTATTTATGAAGtgactg                                      <  1:6327290/51‑1 (MQ=255)          aCATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtgactg                                      <  1:2936968/51‑1 (MQ=255)          aCATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtgactg                                      <  1:1170659/51‑1 (MQ=255)           cATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtgactga                                     <  1:2970825/51‑1 (MQ=255)           cATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtgactga                                     <  1:6948030/51‑1 (MQ=255)            aTGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtgactgac                                    <  1:4320562/51‑1 (MQ=255)            aTGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtgactgac                                    >  1:2022505/1‑51 (MQ=255)             tGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACg                                   <  1:6120804/51‑1 (MQ=255)             tGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACg                                   >  1:1933053/1‑51 (MQ=255)             tGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACg                                   >  1:1620321/1‑51 (MQ=255)              ggCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGaa                                             <  1:715427/40‑1 (MQ=255)              ggCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGt                                  >  1:1200667/1‑51 (MQ=255)              ggCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGt                                  >  1:1944709/1‑51 (MQ=255)              ggCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGt                                  <  1:2701372/51‑1 (MQ=255)               gCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTc                                 <  1:4324909/51‑1 (MQ=255)                cGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCt                                >  1:2846674/1‑51 (MQ=255)                  tCATTGCCAGAAGGGCGGATGACCCGTATTTATGAAGTGACTGACGTCTAc                              <  1:4177261/51‑1 (MQ=255)                  tCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGt                                  >  1:2586208/1‑47 (MQ=255)                  tCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTAc                              <  1:2528370/51‑1 (MQ=255)                   cATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACg                             >  1:3254710/1‑51 (MQ=255)                   cATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACg                             <  1:2341456/51‑1 (MQ=255)                   cATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACg                             >  1:3413762/1‑51 (MQ=255)                   cATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACg                             <  1:3262326/51‑1 (MQ=255)                    aTTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACgg                            >  1:2153665/1‑51 (MQ=255)                      tGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCg                          >  1:6594162/1‑51 (MQ=255)                        ccAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGtc                        <  1:855451/51‑1 (MQ=255)                         cAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGtct                       >  1:5407720/1‑51 (MQ=255)                         cAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGtct                       >  1:2792994/1‑51 (MQ=255)                         cAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGtct                       >  1:1657157/1‑51 (MQ=255)                           gAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTcc                     >  1:3816725/1‑51 (MQ=255)                            aaGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACgg                            >  1:999595/1‑43 (MQ=255)                            aaGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTccc                    <  1:7280870/51‑1 (MQ=255)                            aaGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTccc                    >  1:4423290/1‑51 (MQ=255)                             aGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCg                   <  1:4121632/51‑1 (MQ=255)                               ggCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTa                 <  1:5389555/51‑1 (MQ=255)                                gCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTaa                <  1:2512977/51‑1 (MQ=255)                                gCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTaa                <  1:4048685/51‑1 (MQ=255)                                 cGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAAt               >  1:3949746/1‑51 (MQ=255)                                 cGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAAt               <  1:2844199/51‑1 (MQ=255)                                 cGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAAt               >  1:904497/1‑51 (MQ=255)                                 cGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAAt               >  1:1268460/1‑51 (MQ=255)                                  ggATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATc              <  1:4145780/51‑1 (MQ=255)                                  ggATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATc              <  1:2563593/51‑1 (MQ=255)                                    aTGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCat            >  1:2207819/1‑51 (MQ=255)                                    aTGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCat            >  1:5990568/1‑51 (MQ=255)                                     tGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATc              <  1:6380652/48‑1 (MQ=255)                                     tGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCata           <  1:2898349/51‑1 (MQ=255)                                     tGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCata           >  1:1855477/1‑51 (MQ=255)                                      gACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCatat          <  1:3290052/51‑1 (MQ=255)                                        ccAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATgg        >  1:5065752/1‑51 (MQ=255)                                        ccAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATgg        <  1:16226/51‑1 (MQ=255)                                         cAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGt       >  1:5828471/1‑51 (MQ=255)                                         cAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGt       <  1:6863825/51‑1 (MQ=255)                                         cAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGt       <  1:1469752/51‑1 (MQ=255)                                         cAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGt       >  1:6151116/1‑51 (MQ=255)                                          aGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTc      >  1:802255/1‑51 (MQ=255)                                          aGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTc      >  1:6868218/1‑51 (MQ=255)                                           gTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCa     <  1:4402588/51‑1 (MQ=255)                                            tATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCaa    <  1:7200231/51‑1 (MQ=255)                                            tATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCaa    <  1:3892990/51‑1 (MQ=255)                                            tATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCaa    <  1:349986/51‑1 (MQ=255)                                            tATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCaa    >  1:6666121/1‑51 (MQ=255)                                             aTTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCaaa   >  1:1764008/1‑51 (MQ=255)                                             aTTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCaaa   >  1:2036459/1‑51 (MQ=255)                                             aTTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCaaa   <  1:2363268/51‑1 (MQ=255)                                             aTTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCaaa   >  1:6493199/1‑51 (MQ=255)                                              tttATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATc              >  1:3119377/1‑39 (MQ=255)                                              tttATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCaaaa  >  1:4044926/1‑51 (MQ=255)                                                 |                                                CGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTCTGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAA  >  REL606/4384382‑4384477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑