Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,133,104	G→A	R34R (CGC→CGT)	ppc ←	phosphoenolpyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,133,104	0	G	A	100.0%	309.8 / NA	90	R34R (CGC→CGT)	ppc	phosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/43);  total (47/43)

ATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACGCGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAG  >  REL606/4133056‑4133151                                                 |                                                atcatTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTAcacg                                               <  1:4701301/51‑1 (MQ=255)  tcatTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGt                                              <  1:4702747/51‑1 (MQ=255)  tcatTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGt                                              <  1:6676470/51‑1 (MQ=255)  tcatTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGt                                              <  1:518859/51‑1 (MQ=255)   catTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGtt                                             <  1:7176496/51‑1 (MQ=255)   catTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGtt                                             <  1:5191364/51‑1 (MQ=255)    atTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTc                                            <  1:686477/51‑1 (MQ=255)    atTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTc                                            <  1:5455852/51‑1 (MQ=255)     ttGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCa                                           <  1:4578864/51‑1 (MQ=255)     ttGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCa                                           <  1:220616/51‑1 (MQ=255)      tGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCaa                                          <  1:6433959/51‑1 (MQ=255)      tGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCaa                                          <  1:3530542/51‑1 (MQ=255)       gCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAg                                         >  1:4416163/1‑51 (MQ=255)       gCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAg                                         <  1:6739115/51‑1 (MQ=255)        ccAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGa                                        >  1:4745380/1‑51 (MQ=255)        ccAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGa                                        <  1:487463/51‑1 (MQ=255)        ccAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGa                                        <  1:857/51‑1 (MQ=255)         cAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGna                                       <  1:2033305/51‑1 (MQ=255)          aGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAAt                                      <  1:1938848/51‑1 (MQ=255)          aGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAAt                                      >  1:4123320/1‑51 (MQ=255)          aGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAAt                                      >  1:4371764/1‑51 (MQ=255)            cgcgTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAAtgt                                    >  1:1432407/1‑51 (MQ=255)             gcgTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAAtgtg                                   <  1:7277302/51‑1 (MQ=255)              cgTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATgtgt                                  >  1:4199455/1‑51 (MQ=255)              cgTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATgtgt                                  <  1:660090/51‑1 (MQ=255)               gTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGtt                                 <  1:234766/51‑1 (MQ=255)                tGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTtc                                <  1:3769876/51‑1 (MQ=255)                tGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTtc                                >  1:213296/1‑51 (MQ=255)                 gAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTtct                               <  1:4630645/51‑1 (MQ=255)                 gAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTtct                               >  1:548476/1‑51 (MQ=255)                 gAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTtct                               <  1:3144618/51‑1 (MQ=255)                  aaGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTtctc                              <  1:1992477/51‑1 (MQ=255)                  aaGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTtct                               <  1:2898762/50‑1 (MQ=255)                    gATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTccc                            >  1:7120206/1‑51 (MQ=255)                     aTTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCa                           >  1:730025/1‑51 (MQ=255)                     aTTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCa                           <  1:2539415/51‑1 (MQ=255)                     aTTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCa                           >  1:4967799/1‑51 (MQ=255)                      tttCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCaa                          <  1:4070961/51‑1 (MQ=255)                      tttCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCaa                          >  1:4553787/1‑51 (MQ=255)                       ttCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAAc                         <  1:5770667/51‑1 (MQ=255)                       ttCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAAc                         >  1:1970517/1‑51 (MQ=255)                       ttCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAAc                         >  1:6884094/1‑51 (MQ=255)                        tCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACg                        <  1:5974477/51‑1 (MQ=255)                        tCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACg                        >  1:7250322/1‑51 (MQ=255)                        tCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACg                        <  1:7040524/51‑1 (MQ=255)                         cGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGc                       <  1:2868375/51‑1 (MQ=255)                         cGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGc                       <  1:6687240/51‑1 (MQ=255)                         cGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGc                       >  1:2768575/1‑51 (MQ=255)                          gACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCa                      >  1:6282530/1‑51 (MQ=255)                          gACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCa                      <  1:3115792/51‑1 (MQ=255)                          gACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCa                      >  1:683808/1‑51 (MQ=255)                          gACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCa                      <  1:3248089/51‑1 (MQ=255)                           aCAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCAt                     <  1:7244814/51‑1 (MQ=255)                            cAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATc                    >  1:4295360/1‑51 (MQ=255)                            cAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATc                    >  1:842750/1‑51 (MQ=255)                              aCTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCt                  >  1:5474719/1‑51 (MQ=255)                               cTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCtt                 >  1:7058940/1‑51 (MQ=255)                               cTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCtt                 <  1:619597/51‑1 (MQ=255)                               cTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCtt                 <  1:26589/51‑1 (MQ=255)                                ttACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTg                >  1:828228/1‑51 (MQ=255)                                ttACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTg                <  1:3502113/51‑1 (MQ=255)                                tnACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTg                >  1:2237913/1‑51 (MQ=255)                                 tACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGa               >  1:2663331/1‑51 (MQ=255)                                    ggATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATgg            >  1:5642754/1‑51 (MQ=255)                                    ggATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATgg            >  1:6766103/1‑51 (MQ=255)                                      aTAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGtt          >  1:1418719/1‑51 (MQ=255)                                      aTAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGtt          >  1:1280434/1‑51 (MQ=255)                                       tAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGttt         >  1:943976/1‑51 (MQ=255)                                       tAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGttt         >  1:1659011/1‑51 (MQ=255)                                       tAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGttt         >  1:3488510/1‑51 (MQ=255)                                        aGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTtc        >  1:1147856/1‑51 (MQ=255)                                        aGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTtc        >  1:129875/1‑51 (MQ=255)                                         gTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTtct       <  1:2656668/51‑1 (MQ=255)                                         gTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTtct       <  1:2580371/51‑1 (MQ=255)                                          tttCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTtctc      >  1:757152/1‑51 (MQ=255)                                          tttCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTtctc      <  1:3360708/51‑1 (MQ=255)                                          tttCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTtctc      >  1:5984318/1‑51 (MQ=255)                                           ttCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTcc     >  1:642732/1‑51 (MQ=255)                                           ttCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTcc     >  1:1667478/1‑51 (MQ=255)                                           ttCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTcc     >  1:2453948/1‑51 (MQ=255)                                            tCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTccc    >  1:6755169/1‑51 (MQ=255)                                            tCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTccc    >  1:3013094/1‑51 (MQ=255)                                             cTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCa   >  1:6239970/1‑51 (MQ=255)                                             cTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCa   >  1:2109890/1‑51 (MQ=255)                                             cTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCa   >  1:1770875/1‑51 (MQ=255)                                              tACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAg  >  1:2986218/1‑51 (MQ=255)                                              tACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAg  <  1:6950978/51‑1 (MQ=255)                                              tACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAg  <  1:4968437/51‑1 (MQ=255)                                              tACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAg  >  1:2057122/1‑51 (MQ=255)                                              tACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAg  <  1:1295535/51‑1 (MQ=255)                                              tACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAg  >  1:3627624/1‑51 (MQ=255)                                                 |                                                ATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACGCGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAG  >  REL606/4133056‑4133151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑