Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,096,877	G→T	Y232* (TAC→TAA)	glpF ←	glycerol facilitator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,096,877	0	G	T	100.0%	277.4 / NA	79	Y232* (TAC→TAA)	glpF	glycerol facilitator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (45/34);  total (45/34)

TGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCG  >  REL606/4096829‑4096923                                                 |                                               tGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTa                                              >  1:4339373/1‑51 (MQ=255)  gCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTaa                                             <  1:5069512/51‑1 (MQ=255)  gCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTaa                                             >  1:5688306/1‑51 (MQ=255)  gCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTaa                                             >  1:3082456/1‑51 (MQ=255)  gCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTaa                                             >  1:6265354/1‑51 (MQ=255)  gCACCTACAATCACGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTaa                                             >  1:897906/1‑51 (MQ=255)   cACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAg                                            <  1:7021307/51‑1 (MQ=255)   cACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAg                                            >  1:6152900/1‑51 (MQ=255)   cACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAg                                            <  1:553101/51‑1 (MQ=255)   cACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAg                                            >  1:5127828/1‑51 (MQ=255)    aCCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAgg                                           >  1:2193920/1‑51 (MQ=255)    aCCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAgg                                           <  1:2891384/51‑1 (MQ=255)    aCCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAgg                                           <  1:2045528/51‑1 (MQ=255)    aCCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAgg                                           <  1:5024290/51‑1 (MQ=255)     ccTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGa                                          <  1:5184378/51‑1 (MQ=255)      cTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGaa                                         <  1:4948231/51‑1 (MQ=255)      cTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGaa                                         <  1:3316613/51‑1 (MQ=255)      cTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGaa                                         <  1:2901090/51‑1 (MQ=255)       tACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCGGGAATTAAGGAAt                                        <  1:1653684/51‑1 (MQ=39)       tACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAAt                                        <  1:6706202/51‑1 (MQ=255)        aCAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATg                                       <  1:3919317/51‑1 (MQ=255)        aCAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATg                                       >  1:3072083/1‑51 (MQ=255)         cAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGt                                      >  1:2463410/1‑51 (MQ=255)         cAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGt                                      >  1:613577/1‑51 (MQ=255)          aaTCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGtc                                     <  1:5941922/51‑1 (MQ=255)           aTCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGtct                                    >  1:1515796/1‑51 (MQ=255)            tCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGtctc                                   <  1:2053188/51‑1 (MQ=255)             cgcgCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTctct                                  >  1:1996280/1‑51 (MQ=255)              gcgcCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTg                                 >  1:1682711/1‑51 (MQ=255)               cgcCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgc                                >  1:3910204/1‑51 (MQ=255)                gcCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgcc                               >  1:4529083/1‑51 (MQ=255)                 ccAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgccg                              >  1:4149491/1‑51 (MQ=255)                  cAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgccgc                             >  1:3758929/1‑51 (MQ=255)                   aaCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgccgcc                            >  1:3914395/1‑51 (MQ=255)                   aaCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgccgcc                            >  1:4389136/1‑51 (MQ=255)                    aCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGccgccg                           <  1:1913381/51‑1 (MQ=255)                    aCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGccgccg                           >  1:6287005/1‑51 (MQ=255)                     cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          >  1:3271764/1‑51 (MQ=255)                     cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          <  1:3881931/51‑1 (MQ=255)                     cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          >  1:662450/1‑51 (MQ=255)                     cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          <  1:6217498/51‑1 (MQ=255)                     cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          <  1:580761/51‑1 (MQ=255)                     cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          >  1:7178930/1‑51 (MQ=255)                     cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          >  1:7234188/1‑51 (MQ=255)                     cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          <  1:2731770/51‑1 (MQ=255)                       aTAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTa                        >  1:5750247/1‑51 (MQ=255)                        tAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTaa                       >  1:167025/1‑51 (MQ=255)                         aGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTaaa                      <  1:3336111/51‑1 (MQ=255)                          gggCCAAAAAGCGGCACCGGGAATTAAGGAATGTCTCTGCCGCCGGTAAAg                     <  1:2749452/51‑1 (MQ=39)                          gggCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAg                     <  1:3389748/51‑1 (MQ=255)                           ggCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAgg                    >  1:1230717/1‑51 (MQ=255)                            gCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGc                   >  1:1321895/1‑51 (MQ=255)                             ccAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCg                  >  1:7120434/1‑51 (MQ=255)                             ccAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCg                  >  1:6614772/1‑51 (MQ=255)                             ccAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCg                  <  1:1939318/51‑1 (MQ=255)                             ccAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCg                  >  1:1813506/1‑51 (MQ=255)                              cAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGa                 >  1:6267103/1‑51 (MQ=255)                               aaaaaGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTaa                       <  1:2220529/44‑1 (MQ=255)                                aaaaGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACa               <  1:812771/51‑1 (MQ=255)                                aaaaGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACa               >  1:5655497/1‑51 (MQ=255)                                 aaaGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACAt              >  1:457115/1‑51 (MQ=255)                                 aaaGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACAt              <  1:6245935/51‑1 (MQ=255)                                   aGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTg            <  1:5442245/51‑1 (MQ=255)                                     cGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGcc          >  1:4331821/1‑51 (MQ=255)                                     cGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGcc          >  1:6587577/1‑51 (MQ=255)                                     cGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGcc          >  1:3727566/1‑51 (MQ=255)                                      ggCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTg            >  1:6515398/1‑48 (MQ=255)                                      ggCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGccc         <  1:2971293/51‑1 (MQ=255)                                       gCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGcccc        >  1:2561392/1‑51 (MQ=255)                                       gCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGcccc        <  1:313353/51‑1 (MQ=255)                                        cACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCa       >  1:464390/1‑51 (MQ=255)                                         aCCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAg      >  1:279689/1‑51 (MQ=255)                                           cAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGcc    <  1:2378899/51‑1 (MQ=255)                                           cAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGcc    >  1:7237276/1‑51 (MQ=255)                                            aGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGccc   <  1:1680780/51‑1 (MQ=255)                                            aGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGccc   >  1:1283664/1‑51 (MQ=255)                                            aGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGccc   <  1:5205421/51‑1 (MQ=255)                                             ggAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCg  >  1:615106/1‑51 (MQ=255)                                             ggAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCg  <  1:4451029/51‑1 (MQ=255)                                                 |                                               TGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCG  >  REL606/4096829‑4096923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑