Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,338,108	T→G	intergenic (+266/‑63)	prmA → / → yhdG	ribosomal protein L11 methyltransferase/tRNA‑dihydrouridine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,338,108	0	T	G	100.0%	301.6 / NA	79	intergenic (+266/‑63)	prmA/yhdG	ribosomal protein L11 methyltransferase/tRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (44/35);  total (44/35)

AATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATG  >  REL606/3338062‑3338155                                               |                                                aaTTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCg                                             >  1:7029843/1‑51 (MQ=255) aaTTCTTTGATCCATCTCAGAGGATTGGCCAAAGTTTGGCCTTTCAgct                                               >  1:3417836/1‑49 (MQ=255)   ttCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTg                                           >  1:820830/1‑51 (MQ=255)   ttCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTg                                           <  1:2110148/51‑1 (MQ=255)   ttCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTg                                           >  1:7272386/1‑51 (MQ=255)     cTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCa                                         >  1:6634813/1‑51 (MQ=255)      tttGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCaa                                        >  1:4005026/1‑51 (MQ=255)      tttGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCaa                                        >  1:5600599/1‑51 (MQ=255)      tttGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCaa                                        >  1:5515345/1‑51 (MQ=255)      tttGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCaa                                        <  1:1302329/51‑1 (MQ=255)        tGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCaaaa                                      <  1:4186590/51‑1 (MQ=255)        tGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCaaaa                                      <  1:6005848/51‑1 (MQ=255)         gATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCaaaaa                                     >  1:6933179/1‑51 (MQ=255)         gATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCaaaaa                                     >  1:6284589/1‑51 (MQ=255)         gATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCaaaaa                                     >  1:6012645/1‑51 (MQ=255)          aTCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCaaaaaa                                    >  1:3610046/1‑51 (MQ=255)          aTCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCaaaaaa                                    <  1:1076755/51‑1 (MQ=255)          aTCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCaaaaaa                                    >  1:750009/1‑51 (MQ=255)           tCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAAt                                   <  1:6527720/51‑1 (MQ=255)           tCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAAt                                   <  1:1821055/51‑1 (MQ=255)           tCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAAt                                   <  1:1651523/51‑1 (MQ=255)           tCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAAt                                   >  1:4136726/1‑51 (MQ=255)            ccATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATg                                  >  1:3189420/1‑51 (MQ=255)            ccATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATg                                  <  1:4282631/51‑1 (MQ=255)            ccATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATg                                  >  1:1505306/1‑51 (MQ=255)            ccATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATg                                  <  1:5658046/51‑1 (MQ=255)             cATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGc                                 <  1:110619/51‑1 (MQ=255)             cATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGc                                 >  1:501632/1‑51 (MQ=255)               tctcAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGt                               <  1:657164/51‑1 (MQ=255)               tctcAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGt                               >  1:3674234/1‑51 (MQ=255)                ctcAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTa                              >  1:6677476/1‑51 (MQ=255)                ctcAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTa                              >  1:1506506/1‑51 (MQ=255)                ctcAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTa                              <  1:6989878/51‑1 (MQ=255)                ctcAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTa                              >  1:6496147/1‑51 (MQ=255)                 tcAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTaa                             >  1:3974051/1‑51 (MQ=255)                  cAGAGGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAat                            >  1:7035171/1‑51 (MQ=255)                   agagGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAata                           >  1:7226387/1‑51 (MQ=255)                   agagGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAata                           <  1:5684049/51‑1 (MQ=255)                    gagGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAatat                          >  1:2350339/1‑51 (MQ=255)                    gagGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAatat                          <  1:3133352/51‑1 (MQ=255)                    gagGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAatat                          <  1:6457103/51‑1 (MQ=255)                     agGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAAtata                         <  1:6372252/51‑1 (MQ=255)                     agGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAAtata                         <  1:2735984/51‑1 (MQ=255)                     agGATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAAtata                         <  1:2317814/51‑1 (MQ=255)                      ggATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATAc                        <  1:4658381/51‑1 (MQ=255)                       gATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATAcg                       >  1:2167067/1‑51 (MQ=255)                       gATTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATAcg                       <  1:2538251/51‑1 (MQ=255)                        aTTGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATAcgc                      <  1:169549/51‑1 (MQ=255)                         ttGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATAcgcc                     >  1:6776572/1‑51 (MQ=255)                          tGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATAcgccg                    >  1:1198520/1‑51 (MQ=255)                          tGGTCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATAcgccg                    >  1:6320119/1‑51 (MQ=255)                             tCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCt                 >  1:929573/1‑51 (MQ=255)                             tCAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCt                 >  1:5510950/1‑51 (MQ=255)                              cAAAGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCtt                <  1:6534575/51‑1 (MQ=255)                               aaaGTTTGTCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTg               <  1:6632934/51‑1 (MQ=255)                               aaaGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTg               >  1:1103062/1‑51 (MQ=255)                               aaaGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTg               >  1:4811999/1‑51 (MQ=255)                               aaaGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTg               >  1:1096497/1‑51 (MQ=255)                               aaaGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTg               >  1:2712763/1‑51 (MQ=255)                               aaaGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTg               >  1:4146957/1‑51 (MQ=255)                               aaaGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTg               >  1:6546443/1‑51 (MQ=255)                                aaGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGc              <  1:6181748/51‑1 (MQ=255)                                 aGTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCa             <  1:7065580/51‑1 (MQ=255)                                  gTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAg            <  1:3640911/51‑1 (MQ=255)                                  gTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAg            <  1:6170525/51‑1 (MQ=255)                                  gTTTGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAg            <  1:5984621/51‑1 (MQ=255)                                    ttGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTc          <  1:691646/51‑1 (MQ=255)                                     tGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTca         >  1:1072697/1‑51 (MQ=255)                                     tGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTca         <  1:4241879/51‑1 (MQ=255)                                     tGGCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTca         >  1:4572722/1‑51 (MQ=255)                                      ggCCTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTcac        >  1:1218693/1‑51 (MQ=255)                                        ccTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAg      >  1:2243098/1‑51 (MQ=255)                                        ccTTTCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAg      <  1:1202136/51‑1 (MQ=255)                                          tttCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTa    <  1:3579949/51‑1 (MQ=255)                                          tttCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTa    >  1:4361622/1‑51 (MQ=255)                                          tttCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTa    >  1:5908794/1‑51 (MQ=255)                                          tttCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTa    >  1:6637302/1‑51 (MQ=255)                                           ttCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTAt   <  1:7254361/51‑1 (MQ=255)                                            tCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATg  >  1:2602069/1‑51 (MQ=255)                                            tCAGCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATg  <  1:1187364/51‑1 (MQ=255)                                               |                                                AATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATG  >  REL606/3338062‑3338155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑