Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,746,189	G→A	intergenic (‑237/‑152)	ydiJ ← / → ydiK	predicted FAD‑linked oxidoreductase/predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,746,189	0	G	A	100.0%	246.6 / NA	70	intergenic (‑237/‑152)	ydiJ/ydiK	predicted FAD‑linked oxidoreductase/predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/34);  total (36/34)

ATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAA  >  REL606/1746141‑1746236                                                 |                                                aTGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAgttatt                                               <  1:361877/51‑1 (MQ=255) aTGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAgttatt                                               <  1:2922186/51‑1 (MQ=255)  tGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGttattg                                              <  1:5055050/51‑1 (MQ=255)   gCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGttattg                                              <  1:6254597/50‑1 (MQ=255)   gCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTtattgt                                             >  1:4446576/1‑51 (MQ=255)    cAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTa                                            <  1:2145555/51‑1 (MQ=255)     aaCTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTAc                                           >  1:1156474/1‑51 (MQ=255)         tGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACcaca                                       >  1:5399357/1‑51 (MQ=255)         tGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACcaca                                       >  1:4888247/1‑51 (MQ=255)          gAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAt                                      >  1:4829915/1‑51 (MQ=255)          gAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAt                                      >  1:644482/1‑51 (MQ=255)          gAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAt                                      <  1:234694/51‑1 (MQ=255)           aaaTACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAtt                                     <  1:6208476/51‑1 (MQ=255)            aaTACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAttt                                    <  1:1785488/51‑1 (MQ=255)             aTACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAtttt                                   <  1:505745/51‑1 (MQ=255)              tACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAttttt                                  <  1:2304315/51‑1 (MQ=255)              tACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAttttt                                  <  1:6843358/51‑1 (MQ=255)              tACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAttttt                                  <  1:155440/51‑1 (MQ=255)               acgacgAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAtttttt                                 >  1:5043327/1‑51 (MQ=255)               acgacgAGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAtttttt                                 >  1:4152668/1‑51 (MQ=255)                cgacgaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAttttttt                                <  1:1111044/51‑1 (MQ=255)                cgacgaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAttttttt                                >  1:3998687/1‑51 (MQ=255)                cgacgaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAttttttt                                <  1:5290519/51‑1 (MQ=255)                cgacgaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAttttttt                                >  1:6001047/1‑51 (MQ=255)                cgacgaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACAttttttt                                >  1:2965750/1‑51 (MQ=255)                 gacgaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTc                               >  1:81268/1‑51 (MQ=255)                 gacgaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTc                               <  1:3216099/51‑1 (MQ=255)                 gacgaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTc                               >  1:7141676/1‑51 (MQ=255)                 gacgaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTc                               >  1:6686793/1‑51 (MQ=255)                  acgaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCt                              >  1:2182164/1‑51 (MQ=255)                   cgaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTa                             <  1:6016787/51‑1 (MQ=255)                    gaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTaa                            <  1:379515/51‑1 (MQ=255)                    gaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTaa                            <  1:178277/51‑1 (MQ=255)                    gaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTaa                            <  1:6755407/51‑1 (MQ=255)                    gaGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTaa                            >  1:4408772/1‑51 (MQ=255)                     aGTAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAac                           <  1:1150494/51‑1 (MQ=255)                       tAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAacac                         <  1:5281673/51‑1 (MQ=255)                       tAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAacac                         <  1:5244559/51‑1 (MQ=255)                       tAAATCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAacac                         <  1:6624596/51‑1 (MQ=255)                        aaaTCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTa                             >  1:5907417/1‑46 (MQ=255)                        aaaTCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACg                        <  1:5502868/51‑1 (MQ=255)                          aTCGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGcc                      >  1:7299583/1‑51 (MQ=255)                            cGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCa                    >  1:4710949/1‑51 (MQ=255)                            cGTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCa                    >  1:507478/1‑51 (MQ=255)                             gTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCAt                   >  1:2499946/1‑51 (MQ=255)                             gTTTGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCAt                   <  1:7119380/51‑1 (MQ=255)                              tttGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATc                  >  1:1434284/1‑51 (MQ=255)                              tttGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATc                  >  1:19734/1‑51 (MQ=255)                              tttGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATc                  >  1:6509770/1‑51 (MQ=255)                               ttGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCa                 <  1:6531703/51‑1 (MQ=255)                               ttGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCa                 >  1:179921/1‑51 (MQ=255)                               ttGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCa                 <  1:2808266/51‑1 (MQ=255)                               ttGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCa                 >  1:4571923/1‑51 (MQ=255)                                tGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAg                <  1:2367971/51‑1 (MQ=255)                                tGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAg                <  1:2496838/51‑1 (MQ=255)                                tGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAg                >  1:403529/1‑51 (MQ=255)                                tGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAg                >  1:538180/1‑51 (MQ=255)                                tGCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAg                >  1:2288640/1‑51 (MQ=255)                                 gCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGa               <  1:6698695/51‑1 (MQ=255)                                 gCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGa               <  1:1238726/51‑1 (MQ=255)                                 gCGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGa               <  1:1389530/51‑1 (MQ=255)                                  cGTGTTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGaa              >  1:3053201/1‑51 (MQ=255)                                    tgtTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGAAtt            <  1:5566709/51‑1 (MQ=255)                                    tgtTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGAAtt            >  1:6361632/1‑51 (MQ=255)                                     gtTGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGAATTa           >  1:1201938/1‑51 (MQ=255)                                       tGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAg         <  1:3579025/51‑1 (MQ=255)                                       tGCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAg         >  1:5790058/1‑51 (MQ=255)                                        gCCTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAgg        <  1:2482857/51‑1 (MQ=255)                                          cTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGc      <  1:4783673/51‑1 (MQ=255)                                          cTGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGc      <  1:6318387/51‑1 (MQ=255)                                           tGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCa     <  1:6579789/51‑1 (MQ=255)                                           tGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCa     >  1:3740327/1‑51 (MQ=255)                                           tGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCa     >  1:2859805/1‑51 (MQ=255)                                           tGAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCa     >  1:5793422/1‑51 (MQ=255)                                            gAGTTATTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAg    >  1:4061176/1‑51 (MQ=255)                                              gttattGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGaa  >  1:6071844/1‑51 (MQ=255)                                              gttattGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGaa  >  1:6154157/1‑51 (MQ=255)                                                 |                                                ATGCAACTTGAAATACGACGAGTAAATCGTTTGCGTGTTGCCTGAGTTGTTGTACCACATTTTTTTCTAACACGCCCATCAGAATTAAGGGCAGAA  >  REL606/1746141‑1746236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑