BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,915,108	(A)_8→7	intergenic (‑218/‑120)	zwf ← / → yebK	glucose‑6‑phosphate 1‑dehydrogenase/predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,915,101	0	A	.	100.0%	423.3 / NA	86	intergenic (‑211/‑127)	zwf/yebK	glucose‑6‑phosphate 1‑dehydrogenase/predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (38/48); total (38/48)

Only 100 of 101 total aligned reads displayed.

AAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCAC  >  REL606/1915051‑1915147

aaaaaTCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGa                                                <  1:4206690/51‑1 (MQ=255)

aaaaaTCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGa                                                <  1:3263605/51‑1 (MQ=255)

aaaaaTCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGa                                                <  1:4915952/51‑1 (MQ=255)

 aaaaTCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaa                                               <  1:5350240/51‑1 (MQ=255)

 aaaaTCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaa                                               >  1:3231847/1‑51 (MQ=255)

  aaaTCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaa                                              <  1:1402362/51‑1 (MQ=255)

   aaTCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaa                                             <  1:2487136/51‑1 (MQ=255)

    atcatcGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaa                                            <  1:3916357/51‑1 (MQ=255)

    atcatcGTGTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaa                                            <  1:6419962/51‑1 (MQ=255)

     tcatcGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaa                                           <  1:1727439/51‑1 (MQ=255)

        tcGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaaata                                        <  1:4365813/51‑3 (MQ=255)

        tcGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaa                                              >  1:6833857/1‑45 (MQ=255)

         cGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaaataa                                       <  1:5155213/51‑4 (MQ=255)

         cGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaaataa                                       >  1:6342488/1‑48 (MQ=255)

          gTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaaataac                                      <  1:4734050/51‑5 (MQ=255)

           ttttCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATaaca                                    <  1:643904/51‑1 (MQ=255)

           ttttCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATaaca                                    <  1:7255917/51‑1 (MQ=255)

           ttttCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATaaca                                    >  1:1882021/1‑51 (MQ=255)

            tttCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATaacaa                                   >  1:1821478/1‑51 (MQ=255)

             ttCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATaacaac                                  <  1:4545611/51‑1 (MQ=255)

             ttCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATaacaac                                  <  1:557686/51‑1 (MQ=255)

              tCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACt                                 <  1:3413611/51‑1 (MQ=255)

              tCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACt                                 <  1:3904625/51‑1 (MQ=255)

                cATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACttt                               <  1:1905892/51‑1 (MQ=255)

                cATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACttt                               >  1:4668222/1‑51 (MQ=255)

                 aTTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACtttt                              >  1:2138802/1‑51 (MQ=255)

                 aTTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACtttt                              >  1:4509089/1‑51 (MQ=255)

                 aTTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACtttt                              <  1:5729038/51‑1 (MQ=255)

                 aTTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACtttt                              <  1:7292732/51‑1 (MQ=255)

                 aTTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACtttt                              >  1:3559491/1‑51 (MQ=255)

                  ttCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACttttt                             >  1:6535620/1‑51 (MQ=255)

                   tCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACtttttt                            <  1:3812821/51‑1 (MQ=255)

                   tCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACtttttt                            >  1:2682950/1‑51 (MQ=255)

                    cGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTa                           <  1:1355123/51‑1 (MQ=255)

                    cGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTa                           <  1:5485934/51‑1 (MQ=255)

                     ggTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTAt                          <  1:3988862/51‑1 (MQ=255)

                     ggTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTAt                          <  1:7168370/51‑1 (MQ=255)

                     ggTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTAt                          >  1:5007073/1‑51 (MQ=255)

                      gTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATc                         >  1:5534625/1‑51 (MQ=255)

                      gTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATc                         >  1:4694750/1‑51 (MQ=255)

                       tAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCt                        <  1:1072927/51‑1 (MQ=255)

                       tAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCt                        <  1:2921667/51‑1 (MQ=255)

                       tAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCt                        <  1:541898/51‑1 (MQ=255)

                       tAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCt                        <  1:2854384/51‑1 (MQ=255)

                        aaaaaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTg                       <  1:6881762/51‑1 (MQ=255)

                        aaaaaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTg                       <  1:6784808/51‑1 (MQ=255)

                         aaaaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGc                      >  1:1976768/1‑51 (MQ=255)

                         aaaaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGc                      <  1:1140693/51‑1 (MQ=255)

                          aaaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCt                     <  1:6976104/51‑1 (MQ=255)

                          aaaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCt                     <  1:2526279/51‑1 (MQ=255)

                           aaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCtt                    >  1:343708/1‑51 (MQ=255)

                            aTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCttt                   >  1:4888048/1‑51 (MQ=255)

                            aTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCttt                   <  1:1618405/51‑1 (MQ=255)

                            aTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCttt                   >  1:2027466/1‑51 (MQ=255)

                            aTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCttt                   <  1:5300930/51‑1 (MQ=255)

                            aTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCttt                   >  1:3053158/1‑51 (MQ=255)

                            aTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCttt                   <  1:3673137/51‑1 (MQ=255)

                             tCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCtttt                  >  1:3608990/1‑51 (MQ=255)

                             tCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCtttt                  <  1:1315629/51‑1 (MQ=255)

                              cTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTg                 >  1:4176644/1‑51 (MQ=255)

                              cTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTg                 <  1:4475824/51‑1 (MQ=255)

                              cTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTg                 <  1:1761913/51‑1 (MQ=255)

                              cTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTg                 <  1:1853080/51‑1 (MQ=255)

                              cTGACACTGAACATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTg                 >  1:6822515/1‑51 (MQ=38)

                               tGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGt                <  1:4724122/51‑1 (MQ=255)

                               tGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGt                >  1:3227758/1‑51 (MQ=255)

                                gACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTc               <  1:2085975/51‑1 (MQ=255)

                                 acacTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCa              >  1:2825490/1‑51 (MQ=255)

                                  cacTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCAt             <  1:5427186/51‑1 (MQ=255)

                                  cacTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCAt             <  1:1971438/51‑1 (MQ=255)

                                   acTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCAtt            <  1:6189254/51‑1 (MQ=255)

                                   acTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCAtt            <  1:18501/51‑1 (MQ=255)

                                   acTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCAtt            >  1:6753626/1‑51 (MQ=255)

                                    cTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTa           <  1:4512664/51‑1 (MQ=255)

                                    cTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTa           >  1:5864407/1‑51 (MQ=255)

                                     tGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTaa          >  1:975400/1‑51 (MQ=255)

                                     tGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTaa          <  1:5766047/51‑1 (MQ=255)

                                     tGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTaa          >  1:2194926/1‑51 (MQ=255)

                                     tGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTaa          <  1:7202753/51‑1 (MQ=255)

                                     tGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTaa          >  1:720382/1‑51 (MQ=255)

                                      gATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAac         >  1:5014639/1‑51 (MQ=255)

                                      gATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAac         <  1:4851699/51‑1 (MQ=255)

                                      gATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAac         <  1:168760/51‑1 (MQ=255)

                                       aTCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAaca        <  1:5665026/51‑1 (MQ=255)

                                        tCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAacac       >  1:1309815/1‑51 (MQ=255)

                                         cATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACg      <  1:3853203/51‑1 (MQ=255)

                                         cATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACg      <  1:2269189/51‑1 (MQ=255)

                                         cATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACg      <  1:4345108/51‑1 (MQ=255)

                                         cATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACg      <  1:5081714/51‑1 (MQ=255)

                                          aTGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACgg     >  1:1127941/1‑51 (MQ=255)

                                          aTGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACgg     >  1:6238553/1‑51 (MQ=255)

                                           tGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGc    >  1:722790/1‑51 (MQ=255)

                                            gTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGca   >  1:805518/1‑51 (MQ=255)

                                            gTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGca   <  1:4068399/51‑1 (MQ=255)

                                            gTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGca   <  1:4401039/51‑1 (MQ=255)

                                             ttATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGcac  >  1:4093388/1‑51 (MQ=255)

                                             ttATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGcac  >  1:3044513/1‑51 (MQ=255)

                                             ttATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGcac  >  1:6591233/1‑51 (MQ=255)

                                             ttATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGcac  >  1:4885206/1‑51 (MQ=255)

                                             ttATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGca   >  1:6575784/1‑50 (MQ=38)

AAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCAC  >  REL606/1915051‑1915147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`