Predicted mutation
evidence seq id position mutation freq annotation gene description
RA REL606 4,459,658 A→G 100% P315P (CCA→CCG yjgL → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,459,6580AG100.0% 83.9 / NA 23P315P (CCA→CCGyjgLhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/14);  total (9/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCTTAATAATAAGAAAGCTACTGACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTCCGTGATAAATCATATTGATTTTAGCCGCGATCAATCTAAAGATTTTAGCTGCGAT  >  REL606/4459514‑4459802
                                                                                                                                                |                                                                                                                                                
tcttAATAATAAGAAAGCTACTGACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCgtatat                                                                                                                                             <  2:133556/150‑1 (MQ=255)
               aGCTACTGACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAAt                                                                                                                              <  2:251624/150‑1 (MQ=255)
                 cTACTGACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATAc                                                                                                                            <  1:319503/150‑1 (MQ=255)
                              aaTTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGttt                                                                                                               >  1:531475/1‑150 (MQ=255)
                                  aaTGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTtaat                                                                                                           <  1:211514/150‑1 (MQ=255)
                                            aaGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGc                                                                                                 >  2:122315/1‑150 (MQ=255)
                                                      gTCTCATATTCTTATTGATTCCTTAGATGATGCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTgg                                                                                       >  1:656509/1‑150 (MQ=255)
                                                              ttCTTATTGATTCCTTAGATGATGCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTg                                                                               >  2:312205/1‑150 (MQ=255)
                                                                 ttATTGATTCCTTAGATGATGCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGaaa                                                                            <  2:533015/150‑1 (MQ=255)
                                                                            ttAGATGATGCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTat                                                                 <  2:658441/150‑1 (MQ=255)
                                                                               gatgatGCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTatgca                                                              <  2:88322/150‑1 (MQ=255)
                                                                                   atgCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCt                                                          >  2:170561/1‑150 (MQ=255)
                                                                                    tgCACAAGTTAACCGTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTc                                                         <  1:431943/150‑1 (MQ=255)
                                                                                    tgCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTc                                                         <  2:212038/150‑1 (MQ=255)
                                                                                          aGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTCCGTGAt                                                   <  1:622958/150‑1 (MQ=255)
                                                                                               aCCTTACCCCGGTCATCCATTCGATTCTGGAGACTTTTTTAGAATCCCCGTATATTAATGATGTAAGAATACTAGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTCCGTGATAAATc                                              <  1:437481/150‑1 (MQ=255)
                                                                                               aCCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTACTGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTCCGTGATAAATc                                              >  2:438724/1‑150 (MQ=255)
                                                                                               aCCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTCCGTGATAAATc                                              >  2:315689/1‑150 (MQ=255)
                                                                                                         ggTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTCCGTGATAAATCATATTGAttt                                    >  1:542560/1‑150 (MQ=255)
                                                                                                             aTCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTCCGTGATAAATCATATTGATTTTAGc                                <  1:121960/150‑1 (MQ=255)
                                                                                                             aTCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTCCGTGATAAATCATATTGATTTTAGc                                <  2:544135/150‑1 (MQ=255)
                                                                                                                              gaCTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTCCGTGATAAATCATATTGATTTTAGCCGCGATCAATCTAAAGa               <  1:314798/150‑1 (MQ=255)
                                                                                                                              gaCTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTCCGTGATAAATCATATTGATTTTAGCCGCGATCAATCTAAAGa               <  1:170079/150‑1 (MQ=255)
                                                                                                                                           tCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTCCGTGATAAATCATATTGATTTTAGCCGCGATCAATCTAAAGATTTTAGCTGCGAt  >  1:34406/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TCTTAATAATAAGAAAGCTACTGACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAAGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAATGCAATATTTTGGTGATTCTGAAAAGATAAAGTATGCATGCTCCGTGATAAATCATATTGATTTTAGCCGCGATCAATCTAAAGATTTTAGCTGCGAT  >  REL606/4459514‑4459802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: