Predicted mutation
evidence seq id position mutation freq annotation gene description
RA REL606 4,133,104 G→A 100% R34R (CGC→CGT)  ppc ← phosphoenolpyruvate carboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,133,1040GA100.0% 139.6 / NA 42R34R (CGC→CGT) ppcphosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/26);  total (16/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACGCGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGACACCCCATCTTATCGTTTGATAGCCCTGTATCC  >  REL606/4132965‑4133249
                                                                                                                                           |                                                                                                                                                 
ggTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGaa                                                                                                                                         >  2:228135/1‑150 (MQ=255)
ggTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGaa                                                                                                                                         >  2:228159/1‑150 (MQ=255)
 gTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCCACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTCCTACACGTTCAAGAAt                                                                                                                                        >  1:111521/1‑150 (MQ=255)
 cttaaGGAACTTACTAAACGCACTCGCAACGGGCAACAGCTCGTCGTTCGACAAATTTTGTAAGTTGGTGAGCAACTCCTGGAGGTTATCATCCTTGCCCGCTCGTGAAGATTTCGACAACTTACGTATAGTTTCTAAACGTTGAAGAAt                                                                                                                                        >  1:134659/5‑150 (MQ=255)
          cTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTcc                                                                                                                               <  2:568539/150‑1 (MQ=255)
            gACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCa                                                                                                                             >  1:291693/1‑150 (MQ=255)
               tacacgcacgcGCACCGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACg                                                                                                                          <  1:40148/147‑1 (MQ=255)
                                        cgcgtctgtCGACAAATTGTGTTAGGTGGTGGGCAACTCCTGGCGGTTAGCGTCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGGTCTAGGATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCAc                                                                                                 <  1:341968/142‑1 (MQ=255)
                                        cTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCAc                                                                                                 <  1:508935/150‑1 (MQ=255)
                                                  gagacagtttGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGc                                                                                       <  1:205613/143‑1 (MQ=255)
                                                  gACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGc                                                                                       <  2:650377/150‑1 (MQ=255)
                                                    cAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCAt                                                                                     <  1:365195/150‑1 (MQ=255)
                                                         tttGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGcctgtctc                                                                                >  2:206207/1‑143 (MQ=255)
                                                           tGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACa                                                                              <  1:267871/150‑1 (MQ=255)
                                                            gTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACAt                                                                             <  2:292523/150‑1 (MQ=255)
                                                             tAAGGTTGTGGGTATCTCCTGGCGTTTAGCATCATTGCCAGTGCGTGAAGATTTCGACAACTTCCGGATAGTTTCTACACGTTCAAGAAGGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACAtt                                                                            <  1:82665/150‑1 (MQ=255)
                                                                ggtggtGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATtact                                                                         >  1:667123/1‑150 (MQ=255)
                                                                    gtgAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCCAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGc                                                                     <  2:408899/150‑1 (MQ=255)
                                                                    gtgAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGc                                                                     >  2:1383/1‑150 (MQ=255)
                                                                    gtgAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGc                                                                     <  2:338429/150‑1 (MQ=255)
                                                                      gAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCaa                                                                   >  2:407844/1‑150 (MQ=255)
                                                                              ccTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAat                                                           <  1:574418/150‑1 (MQ=255)
                                                                                   cGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTgt                                                      <  1:99541/150‑1 (MQ=255)
                                                                                     gTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTgttc                                                    >  2:308005/1‑150 (MQ=255)
                                                                                            tcatTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCata                                             >  2:621843/1‑150 (MQ=255)
                                                                                                      cgcgTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGa                                   >  2:673477/1‑150 (MQ=255)
                                                                                                       gcgTGACGATTTCGACAACTTACGGATAGTTTCTACACGTTCCAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGac                                  <  1:459554/150‑1 (MQ=255)
                                                                                                       gcgTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGac                                  <  1:1374/150‑1 (MQ=255)
                                                                                                       gcgTGAAGATTTCGACAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGac                                  <  2:593050/150‑1 (MQ=255)
                                                                                                       gcgTGAAGATTTCGACAACTTACGGATAGTTCCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGac                                  <  2:111853/150‑1 (MQ=255)
                                                                                                       gccggAAGATTTCGACATCTTACGGCTAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGac                                  <  1:186139/146‑1 (MQ=255)
                                                                                                                gttCGTCAACTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTGTCTCCCAGCACTTTGCCGAGCATACTGACATTACTCCGCAATGCGGAATATTGTTCGTTCATATTACCCCAGACACCCCGTCt                         <  1:284597/149‑1 (MQ=255)
                                                                                                                         cTTACGGATAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGGGGAATATTGTTCGTTCATATTACCCCAGACACCCCATCTTATCGTTTg                >  1:180059/1‑150 (MQ=255)
                                                                                                                         cTTACGGATAGTTTCTACACGTTAAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGAATACTGACATTACTAAGCAATGCGGAATATTGTTCGTTCATATTACCTAAGAAACCCCATCTTATCGTTTg                >  1:524248/1‑150 (MQ=255)
                                                                                                                                aTAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGACACCCCATCTTATCGTTTGATAGccc         <  1:406675/150‑1 (MQ=255)
                                                                                                                                aTAGTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGACACCCCATCTTATCGTTTGATAGccc         <  2:378895/150‑1 (MQ=255)
                                                                                                                                   gTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGACACCCCATCTTATCGTTTGATAGCCCTGt      >  2:565815/1‑150 (MQ=255)
                                                                                                                                   gTTTCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGACACCCCATCTTATCGTTTGATAGCCCTGt      >  2:239134/1‑150 (MQ=255)
                                                                                                                                     ttCTACACGTTCAAGAATGTGTTCTCCGAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGACACCCCATCTTATCGTTTGATAGCCCTGTAt    <  2:414698/150‑1 (MQ=255)
                                                                                                                                     ttCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCGCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGACACCCCATCTTATCGTTTGATAGCCCTGTAt    <  1:238459/150‑1 (MQ=255)
                                                                                                                                     ttCTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGACACCCCATCTTATCGTTTGATAGCCCTGTAt    <  2:669092/150‑1 (MQ=255)
                                                                                                                                       cTACACGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGACACCCCATCTTATCGTTTGATAGCCCTGTATcc  <  1:227511/150‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
GGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCAGCGCGTGAAGATTTCGACAACTTACGGATAGTTTCTACGCGTTCAAGAATGTGTTCTCCCAACGCATCCTTGATGGTTTCTCCCAGCACTTTGCCGAGCATACTGACATTACTACGCAATGCGGAATATTGTTCGTTCATATTACCCCAGACACCCCATCTTATCGTTTGATAGCCCTGTATCC  >  REL606/4132965‑4133249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑