| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 4,101,091 | T→C | 100% | E47G (GAA→GGA) | hslU ← | ATP‑dependent protease ATP‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,101,091 | 0 | T | C | 100.0% | 98.0 / NA | 27 | E47G (GAA→GGA) | hslU | ATP‑dependent protease ATP‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/15); total (12/15) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCCTTACCGACGTAGCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCA > REL606/4100947‑4101220 | tCCTTACCGACGTAGCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATgg < 2:483584/150‑1 (MQ=255) ttACCGACGTAGCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGgcgc < 2:479280/150‑1 (MQ=255) aCGTAGCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGctct > 2:419326/1‑150 (MQ=255) aCGTAGCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGctct > 2:559461/1‑150 (MQ=255) aCGGAGCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCTTTCGCCAGCTTAGCCAGCCGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCCTTAGGATAGTTAGCGGGGTCCCTCCTTGGCGCAGccct > 1:186167/1‑150 (MQ=255) ccctcgtCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCTCATTCGCCAGCGTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTg < 1:147456/144‑1 (MQ=255) cTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGTCGCAGCTCTTAGTCGAGCt > 1:303078/1‑150 (MQ=255) cGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCACCTCTTCGTTGCGCTGCa > 1:268630/1‑150 (MQ=255) gAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCg > 1:520915/1‑150 (MQ=255) ataagagacagCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCg < 1:515917/140‑1 (MQ=255) cTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCgctgtctcttat > 2:517397/1‑139 (MQ=255) aCTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGc < 1:669754/150‑1 (MQ=255) aCTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGc < 2:440955/150‑1 (MQ=255) ttGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCaga > 1:617232/1‑150 (MQ=255) ttCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATGGCCACAGaa > 1:171876/1‑150 (MQ=255) ttCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGaa < 2:353919/150‑1 (MQ=255) tAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGt > 2:537546/1‑150 (MQ=255) gCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTg > 2:499326/1‑150 (MQ=255) aGACGACGTGCGATGTCTGTTTTACCGGCACCGGTCGGGCCGGTCCTCAGGATATTTTTCGGGTGCCCTCCATGGCTCAGCTCTTCGTTGGGCTGCATGCGACGCCAGCGGTTACTCAGAGCAATCGCCAGAGAACGCTTGGCGGTGTcc < 1:85340/150‑1 (MQ=255) aGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTcc < 2:522415/150‑1 (MQ=255) aGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTcc < 2:125958/150‑1 (MQ=255) acgGTCTATTTCAGGTTTACCGCCTCCGGTCGGGCCGATCATCAGGATAGTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGcc < 1:514585/150‑1 (MQ=255) cGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTGCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCgatga < 1:418136/150‑1 (MQ=255) gTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTa > 2:256215/1‑150 (MQ=255) ttACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATcc < 2:533420/150‑1 (MQ=255) ttACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATcc < 2:619041/150‑1 (MQ=255) aCCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAg < 2:339337/150‑1 (MQ=255) aCCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAg < 2:542266/150‑1 (MQ=255) ggTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGAc > 1:8812/1‑150 (MQ=255) ggATATTTTTCGGGGTCACTCCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCa > 1:273533/1‑150 (MQ=255) | TCCTTACCGACGTAGCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCA > REL606/4100947‑4101220 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |