| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 430,835 | C→T | 100% | intergenic (‑48/‑108) | insL‑2 ← / → lon | putative transposase insL for insertion sequence IS186/DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 430,835 | 0 | C | T | 92.0% | 66.6 / ‑0.9 | 25 | intergenic (‑48/‑108) | insL‑2/lon | putative transposase insL for insertion sequence IS186/DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (13/10); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAGCGTTCTGAACGCATTGAAATCCCC > REL606/430712‑430978 | gTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATAc < 2:479958/150‑1 (MQ=255) gAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACg < 1:553545/150‑1 (MQ=255) aTCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACAt > 2:259884/1‑150 (MQ=255) aTCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGGAAACATCCCCATATACTGACGTACAt > 2:681586/1‑150 (MQ=255) ttcttcGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAAt < 2:568687/150‑1 (MQ=255) gtcttcGTGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGGAAACATCCCCATATACGGACGTACATGTTAAt < 1:679588/149‑1 (MQ=255) cttcTGGCATACCCATATGGGCCAGAATTGCTGGCAAGATATCGTGAGAGTAATTGATCGGCACGTTACATCATATCAGGCGCAAGACCACAACCCTTAAGTTAGTGCTTATGGGGAAACGTCCCCATATACTGACGTACATGTTGATAg < 1:137/150‑1 (MQ=255) tACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGa > 2:590525/1‑150 (MQ=255) tACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGa > 2:672561/1‑150 (MQ=255) tACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGa > 2:514070/1‑150 (MQ=255) tACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGa > 2:380774/1‑150 (MQ=255) cAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGc < 1:379670/150‑1 (MQ=255) cAGAATTGCTGACGAGTTAGCGTGAGAGTATTTCATCGGAACGTTAAAACATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACAGCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCgtgt < 1:609850/150‑1 (MQ=21) gAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCa < 1:259143/150‑1 (MQ=255) gAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCAAGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCa < 1:588789/150‑1 (MQ=255) gccAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTAc < 2:546648/149‑1 (MQ=255) ccAGTTATCGTGAGAGTAATTCATCGGCACGTTTAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATTGCGTGAAGCACAGTCGTGTCATATGATTAcc > 1:545068/1‑150 (MQ=255) ttAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTAt < 1:670581/150‑1 (MQ=255) ggCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAGCGt > 2:565231/1‑150 (MQ=255) caCCACAGCCCGTCAGTTAGGGCTGCGGGTGCAACATCCTCATGTACTGACGTAGATGAGAATAGATGGCGTGAAGCACGGTCGTGTCATCTGCGTACCTGGCGGAAATTAAGCTAAGAGAGAGCTCTATGAATCCTGAGCGTTCTGAAc < 1:172603/149‑1 (MQ=255) aCCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCCATGAATCCTGAGCGTTCTGAACGGATTGa > 1:243392/1‑150 (MQ=255) cccTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAGCGTTCTGAACGCATTGaa > 2:563776/1‑150 (MQ=255) cccTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAGCGTTCTGAACGCATTGaa > 2:178005/1‑150 (MQ=255) ccTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAGCGTTCTGAACGCATTGaaa > 2:274470/1‑150 (MQ=255) ccTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAGCGTTCTGAACGCATTGaaa > 2:282064/1‑150 (MQ=255) aGTTAGTGCTTATGGGGAAACATCCGCATATACTGACGTACATGTTAATAGATGGCGTGCAGCACAGTCGTGTCATCTGATTACCTTGCGGAAATTAGACTCAGATAGAGCTTCAAAAATCCTGAGGGATCTTAAAGCGTTTAAATccca > 1:682105/1‑149 (MQ=255) | GTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAAGAGAGAGCTCTATGAATCCTGAGCGTTCTGAACGCATTGAAATCCCC > REL606/430712‑430978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |