| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 3,409,316 | T→G | 100% | S41S (TCT→TCG) | yheS → | fused predicted transporter subunits of ABC superfamily: ATP‑binding components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,409,316 | 0 | T | G | 100.0% | 72.6 / NA | 20 | S41S (TCT→TCG) | yheS | fused predicted transporter subunits of ABC superfamily: ATP‑binding components |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (9/11); total (9/11) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTCTGAACATACGGCGCCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCTACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACGCCGGCGTTACCGCAAGCGGCGCTGGAATATGTCATTGACGGAGAC > REL606/3409174‑3409457 | gTCTGAACATACGGCGCCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctgc > 2:97296/1‑150 (MQ=255) aaCATACGGCGCCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCa < 1:97007/150‑1 (MQ=255) gcgcCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGaa > 2:153033/1‑150 (MQ=255) ttACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACggcggc > 2:4619/1‑150 (MQ=255) tcgtcgCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTacac < 2:459850/150‑1 (MQ=255) cgtcgCGGCGTGTGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACAcc < 1:1695/150‑1 (MQ=255) cgtcgCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACAcc < 2:140793/150‑1 (MQ=255) cgtcgCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACAcc < 2:533013/150‑1 (MQ=255) gATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCg > 2:12041/1‑150 (MQ=255) agacagccgccaccATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTggg < 2:51470/144‑1 (MQ=255) ccgccaccATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTgggctgtct > 1:51316/1‑144 (MQ=255) ccgccaccATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATc > 2:23066/1‑150 (MQ=255) caTCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATCGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAAc > 2:639998/1‑150 (MQ=255) cAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACGcc < 2:217838/150‑1 (MQ=255) aGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACGCCGGCGTTACCGc < 2:378132/150‑1 (MQ=255) gtggGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACGCCGGCGTTACCGCAAGCGGCGCTGGaa < 1:544731/150‑1 (MQ=255) gtggGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACGCCGGCGTTACCGCAAGCGGCGCTGGaa < 1:12001/150‑1 (MQ=255) gggTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACGCCGGCGTTACCGCAAGCGGCGCTGGAata < 1:152600/150‑1 (MQ=255) gTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACGCCGGCGTTACCGCAAGCGGCGCTGGAATATg > 2:138574/1‑150 (MQ=255) tgTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAGCGCCGGCGTTACCGCAAGCGGCGCTGGAATATGGCATTGACGGAGAc < 1:203337/149‑1 (MQ=255) ggTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACGCCGGCGTTACCGCAAGCGGCGCTGGAATATGTCATTGACGGAGAc > 1:562258/1‑150 (MQ=255) | GTCTGAACATACGGCGCCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCTACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACGCCGGCGTTACCGCAAGCGGCGCTGGAATATGTCATTGACGGAGAC > REL606/3409174‑3409457 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |