| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 3,308,106 | G→A | 100% | A6A (GCG→GCA) | yhcB → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,308,106 | 0 | G | A | 100.0% | 60.0 / NA | 21 | A6A (GCG→GCA) | yhcB | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/10); total (11/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTT > REL606/3307959‑3308247 | gTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCACt > 2:99897/1‑150 (MQ=255)gTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCACt > 2:35168/1‑150 (MQ=255) atatGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCACTAATTGGGTTAgtcgt > 2:557742/1‑150 (MQ=255) ttCCACTTCTGTGGAATTTACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCACTCATTGGGTTAGTCGTCGGCGTCATTATTg > 1:200818/1‑150 (MQ=255) ttCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCACTAATTGGGTTAGTCGTCGGCATCATTATTg > 1:432428/1‑150 (MQ=255) ttCCACTTCTGTGGAATTAACGGTTATAGTGAAATAATCAGCCGCAGGCATGGAGCATGAAGCCAACACCCTACGGAAAAAAAAGACAACGGGAGATGTTAATGACCAGGGAATATGCACTAATCGGGTTAGTCGCAGGAATCAttgttg > 1:317381/1‑150 (MQ=255) tCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCACTAATTGGGTTAGTCGTCGGCATCATTATTgg < 2:321617/150‑1 (MQ=255) ttCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCACTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCtg < 2:186762/150‑1 (MQ=255) taGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCACTAACTGGGGTAGTCGTCGGCATCATTATTGGTGCTGTGGCCAGGCGGTTTGGAAAt > 1:69491/1‑150 (MQ=255) aTAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCACTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAAc < 2:103822/150‑1 (MQ=255) atgtagacggacaccAACACCCTACGTTAACAAAAGAGAACGGGATAGGGTTATGACCGTGGGGTATGCACGGATGCGGTTAGTCTGTGTCATCATTATTGGTGCTGTGGCCATGGGTTTTGGTAATCGTAAACTACGCGACCAACAGGc < 1:595762/137‑1 (MQ=255) aTGGAGCCTGAAGCCCACACCCTACGGAAACATCAGCGAACGGTGGATGTTCATGACCTGGTAATATGCACTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCGAGCAACAGGc < 1:565346/150‑1 (MQ=255) aGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCACTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTg > 2:351851/1‑150 (MQ=255) gCCTGAAGCCAACACCCTACGGCAACAAAAGACAACGGGAGAAGTTCATGACCTGGGAATATGCACCAATTGGGTGAGTCGTCGGCATCATTATTGGTGCTGTGGCCACGCGTCTTGGAAATCGGAAACTCCGCCAGCACCAGGCGTTGc > 1:295219/1‑150 (MQ=255) aaGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCACTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTAc < 2:13020/150‑1 (MQ=255) tccGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCAATAATTGGGTGAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAg < 1:21467/148‑1 (MQ=255) gaaaaGACAACGGGGGCTGTTCATGACTTGGGAATATGCAATAATTGGGTTAGTCGTCGGCATCATGATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGc < 1:675990/149‑1 (MQ=255) aGACAACGGGAGATGTTCATGACCTGGGAATATGCACTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGGTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGATGaa > 1:75056/1‑150 (MQ=255) cAACGGGAGATGTTCATGACCTGGGAATATGCACTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTg < 1:481468/150‑1 (MQ=255) cGGGAGATGTTCATGACCTGGGAATATGCACTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGAc < 1:584438/150‑1 (MQ=255) ttCATGACCTGGGAATATGCACTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATcgc > 2:226422/1‑150 (MQ=255) taaTATGCACTATTTGGATTAGTTGTTGGCATGATGATTGGTGTGGCGGCCATGCCGTGTGGTAATCGTAAACTACGCCAGAAACAGGCGTTGCAGTACGAACTGTAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGtt < 1:302758/149‑1 (MQ=255) | GTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTT > REL606/3307959‑3308247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |