| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 3,093,020 | T→G | 100% | F74L (TTT→TTG) | yqhH → | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,093,020 | 0 | T | G | 100.0% | 46.9 / NA | 15 | F74L (TTT→TTG) | yqhH | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (11/4); total (11/4) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGGCGAGCAATGTGCAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCG > REL606/3092883‑3093160 | atGGCGAGCAATGTGCAAACATTAAATGCCAAAAGCGCCCGGCTTGAGCAGGATATGAAAGAACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTgcgc < 1:199066/149‑1 (MQ=255) ggCGAGCAATGTGCAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCgctg > 2:418984/1‑150 (MQ=255) ggCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATTCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATTATAAAAAAATCCCCGGt > 2:155104/1‑150 (MQ=255) cTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTTACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAg < 1:154666/150‑1 (MQ=255) cTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAg < 2:451056/150‑1 (MQ=255) ggATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAg > 1:623558/1‑150 (MQ=255) ggATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAg > 2:243104/1‑150 (MQ=255) ggATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAg > 2:631083/1‑150 (MQ=255) ggATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAg > 2:670366/1‑150 (MQ=255) ggATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAg > 2:530836/1‑150 (MQ=255) gagCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTCGCCGGGGATATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCa > 1:187690/1‑150 (MQ=255) gTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGAATATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCAcc < 1:529304/150‑1 (MQ=255) gCTCAGGCTTATTGGGAATGCCTGCGCTGCTTGCGTATGTCCGCAGAATGATAAACAAATCCCCGGTAGCGTGTCAGTTCCCCGGGATATTTCTTATCGTCCAACCGCCGCTTTAGGCCGTCTCTTCCGACCAGCAGTCCCCGGCCggga > 1:130806/1‑150 (MQ=255) gCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACgaga > 2:299105/1‑150 (MQ=255) gCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACgaga > 2:166622/1‑150 (MQ=255) ggACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCg > 2:524617/1‑150 (MQ=255) | CTGGCGAGCAATGTGCAAACATTAAATGCCAAAATCGCCCGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAACCGCCGCTTTAGGCCGTTTCTTCGCACCAGCATTCACCGGACGAGATTGCG > REL606/3092883‑3093160 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |