Predicted mutation
evidence seq id position mutation freq annotation gene description
RA REL606 2,758,314 A→C 100% K374Q (AAA→CAA)  ygbK → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,758,3140AC93.6% 153.4 / 1.5 47K374Q (AAA→CAA) ygbKhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base C (23/21);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATAAAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAG  >  REL606/2758170‑2758458
                                                                                                                                                |                                                                                                                                                 
cAGCACTACGGTGCACAAACAGCCAGGCAGGCAGTAGAAACACTGTCTTCTCAACTAGCGGCGCGGTTAGCAGCGGACGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGGCCTCCGGCGGAGGCACACAGAGCCTGGGAATACAAggg                                                                                                                                              <  1:332503/150‑1 (MQ=255)
cAGCAATACGGTGCACAAACAGCCAGTCAGGTAGTAGAAACACTGTTTTCTCAACTAGCGGCGCGGATAGCAGCGGAAGGCGTGATACGCTTTATTGTAGCAGGCGGTGAGACCTCAGGCGTAGTCACACAGAGCCTGGGAATACAAggg                                                                                                                                              >  1:636619/1‑150 (MQ=255)
cAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAggg                                                                                                                                              >  2:226450/1‑150 (MQ=255)
cAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAAAACAAggg                                                                                                                                              >  2:313582/1‑150 (MQ=255)
  gCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGtt                                                                                                                                            >  2:41606/1‑150 (MQ=255)
  gCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGtt                                                                                                                                            >  1:277187/1‑150 (MQ=255)
       aCGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCata                                                                                                                                       <  2:138047/150‑1 (MQ=255)
       aCGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCata                                                                                                                                       <  2:603982/150‑1 (MQ=255)
       aCGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATAAAGGGGTTTCata                                                                                                                                       <  2:465654/150‑1 (MQ=255)
               caAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCa                                                                                                                               >  2:420751/1‑150 (MQ=255)
                              gCAGTAGAAACACTGTTTTCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGc                                                                                                                >  2:175064/1‑150 (MQ=255)
                                 gaaGAACCACTGTTTTCTCAACTATCCCCGCGGTTAGCAGCGGAAGGCGGGACACGCCTTAATGTCGCAGGCGGAGCGACCTCCGGCGTAGTCAAACACAGTCGGGGAATACCAGGGATTCATATTTGCCCAACCGTTTCCCCCGGCGTg                                                                                                             >  1:96494/3‑150 (MQ=255)
                                               ttCGCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGc                                                                                               <  1:136016/150‑1 (MQ=255)
                                                         gcggcgCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAg                                                                                     <  2:374218/150‑1 (MQ=255)
                                                          cggcgCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTGCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑c                                                                                   <  1:247336/150‑1 (MQ=255)
                                                           ggcgCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGCGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑cc                                                                                  <  1:422335/150‑1 (MQ=255)
                                                           ggcgCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑cc                                                                                  <  2:406201/150‑1 (MQ=255)
                                                           ggcgCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑cc                                                                                  <  2:447326/150‑1 (MQ=255)
                                                           ggcgCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑cc                                                                                  <  1:121450/150‑1 (MQ=255)
                                                           ggcgCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑cc                                                                                  <  1:419555/150‑1 (MQ=255)
                                                           ggcgCGGTTAGCAGCGGAAGGCGTAACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑cc                                                                                  <  1:321020/150‑1 (MQ=255)
                                                           ggcgCAGTCAGCTGCGGACAGCGTGACACCCCTTATTGTCGCAGGCGGTGCGACCTCCGGCGTAGTCACACAGGGCCGGGGAAGACAAGGGTTTCATGTTGGCCCAACCATGTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑cc                                                                                  <  1:511841/150‑1 (MQ=255)
                                                            gcgcGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCt                                                                                 >  2:418495/1‑150 (MQ=255)
                                                               cgGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGtc                                                                              <  2:638487/150‑1 (MQ=255)
                                                                ggTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGGGCCTGGGGATACAAGGGTTTCATATTGTCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGAACGGGATAAG‑CCTGtct                                                                             >  1:673924/1‑150 (MQ=255)
                                                                ggTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATAAAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGtct                                                                             <  2:536981/150‑1 (MQ=255)
                                                                gacaggcagcGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGtct                                                                             <  2:402033/145‑1 (MQ=255)
                                                                 gTTAGCAGCGGAAGGCGGGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCAAACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGtctc                                                                            <  1:229566/150‑1 (MQ=255)
                                                                   tAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCAc                                                                          >  1:104830/1‑150 (MQ=255)
                                                                     gcagcGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGtctctgtc                                                                        >  1:400875/1‑146 (MQ=255)
                                                                               gCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCCTGtctcttatacacatctc                                                               >  2:9777/1‑137 (MQ=255)
                                                                                cGTGAAACGCGTTATTGTAGCGGGCGGTGAGTCCGCCGGCGTAGTCCCACAGACCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATc                                                             <  1:204844/150‑1 (MQ=255)
                                                                                                gtagCAGGCGGTGAGACATCCGACGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCACGACCCAGTCCCCCGGCGTGCAGTGGGAAAACGCACTGGATAAT‑CCTGTCTAACTCGCCCTTAAATCTGGCAAATTCGGtgtt                                             >  1:178183/4‑148 (MQ=255)
                                                                                                      ggCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGcc                                       >  2:61538/1‑150 (MQ=255)
                                                                                                         ggagagaCCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTATCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAGCGCACTGGATACC‑CGTGTGTCACTCGCCCTTAACTCTGGCCACGTCGgtggtggatcattt                                    >  1:438978/4‑142 (MQ=255)
                                                                                                         ggTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCttt                                    >  2:100240/1‑150 (MQ=255)
                                                                                                                 cTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTCACg                            >  2:595740/1‑150 (MQ=255)
                                                                                                                          aGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTCACGAGCCCAAag                   <  2:105135/150‑1 (MQ=255)
                                                                                                                           gTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTCACGAGCCCAAaga                  >  2:470752/1‑150 (MQ=255)
                                                                                                                            tCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTCACGAGCCCAAagag                 <  1:99944/150‑1 (MQ=255)
                                                                                                                               cacaGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTCACGAGCCCAAAGAGCGt              >  1:526018/1‑150 (MQ=255)
                                                                                                                               cacaGAGCCTGGGAATAAAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTCACGAGCCCAAagagaag              >  1:41358/1‑148 (MQ=255)
                                                                                                                                   gagCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTCACCAGCCCAGCGAGAGttttt          >  1:630926/1‑150 (MQ=255)
                                                                                                                                   gagCCTGGGAATACAAGGGTTTCATATTCGCCCAACCACTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCCCCCTTCAATCTGGCCACTTCGTGCATGACGCCTTTTTTTCCCGCGCCCAAAGAGAGtgctt          >  1:216088/1‑146 (MQ=255)
                                                                                                                                       cagggAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCCCTGGATAAG‑CCAGTCTCACTCGCCCTTAAATCTGGACACTGCGGTGATGACGCCTTTTTTTCACGAGACCAAAGAGTGTTTTTATCa      <  1:208529/148‑1 (MQ=255)
                                                                                                                                       cTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCa      <  2:527533/150‑1 (MQ=255)
                                                                                                                                       cTGGGAATAAGAGGGTCTCATACTGGCCCAACCATTTCCCCCGGCGTGCCGATGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAAGCTGGCAACTTCGGTGATGTCGCCTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCa      <  1:127979/150‑1 (MQ=255)
                                                                                                                                          ggAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGa   >  1:293566/1‑150 (MQ=255)
                                                                                                                                           gAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTCACGCGCCCAAAGAGGGTTTTTATCATGac  >  1:213620/1‑149 (MQ=255)
                                                                                                                                           gAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAg  >  2:65828/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CAGCAATACGGTGCACAAAAAGCCAGTCAGGCAGTAGAAACACTGTTTTCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATAAAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAG‑CCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGTGATGACGCCTTTTTTTCACGAGCCCAAAGAGAGTTTTTATCATGAG  >  REL606/2758170‑2758458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑