| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 875,094 | G→C | 100% | H65D (CAT→GAT) | deoR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 875,094 | 0 | G | C | 100.0% | 37.1 / NA | 12 | H65D (CAT→GAT) | deoR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (6/6); total (6/6) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCG‑CAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATT > REL606/874947‑875243 | tattttataTCGCTTCAACACTCCACTGCAACGTTGTACCCCAGGCAAACAAGGCTGTCTGATCGGGGTCTACCAG‑GTCGTCGGGCAGTTTTGCCGCCCGGCGTTTTGCTTCCGCCGGGGGGGAGTTTTGATCGCTGAGCAGGTAAtcgc < 1:49219/142‑1 (MQ=255) cAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGCTCTGATCGGGTGCTACCAGCGTCG‑CAGCCAGTTTTGCCGCCCGTGGGTTTTCTTACTCCAGGCGGGATTTTTGATCGCTTCACAGGTACTCGCTGGCCCTGCGCGGtt > 1:226901/1‑150 (MQ=255) aggtgtggCCACAGTCAAAAAAGAGGGTCAGATAGGGTTCTACCAGCGTCG‑CAGTGGGTTTTGCCGGCCGGCGTTTTTGTTGCACCAGGCGGGATTTTTGATCGCGTAACAGGTGATCGCTGGCACTGCGCGGGTCCAGAACAATGTAgc < 1:600720/143‑1 (MQ=255) gTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCG‑CAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATCGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAac > 1:182505/1‑150 (MQ=255) gTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCG‑CAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATCGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAac > 2:72467/1‑150 (MQ=255) gATCGGGTTCTACCAGCGTCG‑CAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATCGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGgt > 2:444406/1‑150 (MQ=255) tCGGGTTCTACCAGCGTCG‑CAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATCGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGgttg < 1:443150/150‑1 (MQ=255) ggTTCTACCAGCGTCG‑CAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATCGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTc < 2:183031/150‑1 (MQ=255) ggTTCTACCAGCGTCG‑CAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATCGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTc > 2:614540/1‑150 (MQ=255) gCGTCG‑CAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATCGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGcgac < 2:550081/150‑1 (MQ=255) cccGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATCGCTAGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAAccc < 1:392312/150‑1 (MQ=255) cTTAACAGGTAATCGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGAGGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCg > 1:589637/1‑150 (MQ=255) ttAACAGGTAATCGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGc < 1:612743/150‑1 (MQ=255) aGGTAATCGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGt > 2:217000/1‑150 (MQ=255) ggctggcACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAAtt > 1:517541/1‑150 (MQ=255) | CATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCG‑CAGCCAGTTTTGCCGCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATT > REL606/874947‑875243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |