| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 4,628,460 | T→G | 100% | T3P (ACC→CCC) | arcA ← | DNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,628,460 | 0 | T | G | 100.0% | 78.3 / NA | 24 | T3P (ACC→CCC) | arcA | DNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (12/12); total (12/12) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTAACATGACTAAAGTACGTAATTGCGTTCTTGATGCACTTTCCA > REL606/4628313‑4628601 | tCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCt > 1:3138/1‑150 (MQ=255) ggTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGt > 2:534838/1‑150 (MQ=255) gATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGc < 2:601335/150‑1 (MQ=255) cATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCt > 2:65736/1‑150 (MQ=255) tatTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTaa > 1:279360/1‑150 (MQ=255) ttCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAAt < 1:533292/150‑1 (MQ=255) ttCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAAt > 2:677988/1‑150 (MQ=255) gATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTa > 2:339415/1‑150 (MQ=255) aTGCAGTTCCTCGTCACCTGTCGCTGCGGAATCACCATTGCGTGCCGCTTCGACAATACTTTTCAACGTGCTGCGTGTTCCCAACTCGTTTTCAATGATAAGAATGTGTGGGGGCTGCATGTTTGCTACCTAAATTTCCAACTAAATCGa < 1:1778/150‑1 (MQ=255) aaCATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGAcctgcc < 2:39688/150‑1 (MQ=255) atAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGatgc < 1:676001/150‑1 (MQ=255) gCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGatgcatg > 2:675979/1‑150 (MQ=255) cGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCaa < 2:446246/150‑1 (MQ=255) gAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAAc < 2:266914/150‑1 (MQ=255) cTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCg > 1:323436/1‑150 (MQ=255) cTTTTCAACGTGTTGCGTGTTACCAACTCGATTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTCCAAAAGTCCCTGACCTGCCTGATGCATGCTGCACATAAACATGATCg > 1:447533/1‑150 (MQ=255) tCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGt < 1:673995/150‑1 (MQ=255) cgggtTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTAACa < 1:338442/147‑1 (MQ=255) gtgtTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTAACAt < 2:80231/150‑1 (MQ=255) ttACCAACTCGTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTAACATGACTAAAGTa > 1:174846/1‑150 (MQ=255) gTTTTCAACGATAAGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTAACATGACTAAAGTACGTAATTGCg < 2:175345/150‑1 (MQ=255) aaGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTAACATGACTAAAGTACGTAATTGCGTTCTTGATGCAc < 1:264020/150‑1 (MQ=255) aGAATGTGCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTAACATGACTAAAGTACGTAATTGCGTTCTTGATGCACt > 2:683207/1‑150 (MQ=255) gtgCGGGGGCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTAACATGACTAAAGTACGTAATTGCGTTCTTGATGCACTTTCCa > 2:503840/1‑150 (MQ=255) | TCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAACTCGTTTTCAACGATAAGAATGTGCGGGGTCTGCATGTTTGCTACCTAAATTGCCAACTAAATCGAAACAGGAAGTACAAAAGTCCCTGACCTGCCTGATGCATGCTGCAAATTAACATGATCGGCGTAACATGACTAAAGTACGTAATTGCGTTCTTGATGCACTTTCCA > REL606/4628313‑4628601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |