| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 433,668 | C→T | 100% | R55C (CGT→TGT) | hupB → | HU, DNA‑binding transcriptional regulator, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 433,668 | 0 | C | T | 97.8% | 147.1 / ‑4.0 | 46 | R55C (CGT→TGT) | hupB | HU, DNA‑binding transcriptional regulator, beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (25/20); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGT > REL606/433523‑433808 | gATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGtgc < 1:566592/150‑1 (MQ=255) aCAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGccc < 1:632431/150‑1 (MQ=255) gggCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAAcc < 2:667329/150‑1 (MQ=255) ggCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAAccc > 1:477209/1‑150 (MQ=255) ctctATAGCGGCGGCTGGCCGTACGTTAGATGCTATTATTGCTTCCGTACCTGAATCTTTGCCAGAAGGGGGTGAGGTAGAACTGGTAGGGTTTGGTACTTTTGCCGTTAGAGAGTGTGCTGCGCGTAGTGGTGGCAACCTGCCGACTgg > 1:478922/1‑150 (MQ=255) ctctAAAGCTGCGGCTGGCCGTGCGTTAGATGTTATTATTGCTTCCGTAACTGAATCTCTGAAGGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCTCGTACTGGTCGCAACCCGAAGACCgg > 1:396673/1‑150 (MQ=255) ctctAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGACGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCgg > 1:156198/1‑150 (MQ=255) ctctAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCgg > 2:614799/1‑150 (MQ=255) ctctAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCgg > 1:627197/1‑150 (MQ=255) ctctAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCgg > 2:163846/1‑150 (MQ=255) ctctAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCgg > 1:330633/1‑150 (MQ=255) tAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTaa < 2:397823/150‑1 (MQ=255) aGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAaga > 2:72314/1‑150 (MQ=255) tgtgtataagagacagCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAagag < 1:253501/135‑1 (MQ=255) gCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAagag < 2:331571/150‑1 (MQ=255) gCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAagag < 2:302626/150‑1 (MQ=255) gCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAagag < 2:478563/150‑1 (MQ=255) cTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAgaga < 2:156641/150‑1 (MQ=255) cTGCGGCTGGCCGTGAGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAgaga < 1:613000/150‑1 (MQ=255) aTGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAg < 2:564990/150‑1 (MQ=255) tGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGt < 2:509917/150‑1 (MQ=255) cTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTAc < 2:475039/150‑1 (MQ=255) cTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTAc < 1:332466/150‑1 (MQ=255) cTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTAc < 1:88928/150‑1 (MQ=255) attattGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGCACTGGCCTCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCg > 1:54795/1‑150 (MQ=255) ctctGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCAc < 2:629045/150‑1 (MQ=255) aagaagGGTATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAg < 1:163384/150‑1 (MQ=255) gatgatGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTa > 1:245293/1‑150 (MQ=255) atgTAGCACTGGTAGGTTTTGGTTCTTTTGCCGTTAAAGAGTGTGCTGCCCGTAATAACCGCAACCCGCAGACAGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCATGTAAAGCACTGAACGGCGCGGTCAACt > 1:498789/1‑150 (MQ=255) atgTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAACGACGCGGTAAACt > 1:27900/1‑150 (MQ=255) atgTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACt > 1:538306/1‑150 (MQ=255) atgTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACt > 2:150328/1‑150 (MQ=255) atgTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACt > 2:542732/1‑150 (MQ=255) atgTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACt > 2:503277/1‑150 (MQ=255) atgTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACt > 2:15116/1‑150 (MQ=255) atgTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCACGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAATCACTGAAAGACGCGGTAAACt > 1:146989/1‑150 (MQ=255) cACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTTGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACTAAGCGt < 2:632155/150‑1 (MQ=255) cTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCTCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACTAAGCGTTg > 1:54562/1‑150 (MQ=255) ggTAGGTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGCAAAGAGATCACCATCGCTGCTGCTAAAGCACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACTAAGCGttcta > 1:54336/1‑147 (MQ=255) gTTTTGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACTAAGCGTTGTCCCCAg > 1:453612/1‑150 (MQ=255) ttttGGTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACTAAGCGTTGTCCCCAGt < 1:15067/150‑1 (MQ=255) gTACTTTTGCCGTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACTAAGCGTTGTCCCCAGTGGGGa < 1:451169/150‑1 (MQ=255) ggtAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCCCTGAAAGACGCGGTAAATTAAGCGTTGTCCCCAGGGTGGATGTGACGAAGt > 1:600275/3‑150 (MQ=39) gTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAATGCACTGGCAGAGGCGGTAAACTACGCGTTGTCCCCAGTGGGGATGTGCAGAAGt > 1:628341/1‑150 (MQ=38) gTTAAAGAGTGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGt > 1:27757/1‑150 (MQ=255) gTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGt > 2:666553/1‑150 (MQ=255) | GATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCTGCTAAAGTACCGAGCTTCCGTGCAGGTAAAGCACTGAAAGACGCGGTAAACTAAGCGTTGTCCCCAGTGGGGATGTGACGAAGT > REL606/433523‑433808 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |