Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	REL606	474,374	+TCGCCAGCG	77.0%	coding (932/1677 nt)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 474374	4 (0.170)	15 (0.600) +TCGCCAGCG	11/226	NT	77.0%	coding (932/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
	?	REL606	474375 =	5 (0.190)					coding (931/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGT  >  REL606/474233‑474521                                                                                                                                               |                                                                                                                                                   cccagtcccgccaggataaacgcgaactcaccaatctgcgccaggctggcggcgatggttaatgccgtacgttgggagtgaccaaacagtcgcaccaggaaaaatgcggctaacgacttaccaaacagaataatcgccagcgtcgccagc                                                                                                                                             <  2:438625‑M2/8‑1 (MQ=255)                           ctcaccaagctgcgccaggctggcggcgatggttaatgccgtacgttgggagtgaccaaacagtcgcaccaggaaaaatgcggctaacgacttaccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTg                                                                                                                   <  1:158561‑M2/34‑1 (MQ=255)                            tcaccaatctgcgccaggctggcggcgatggttaatgccgtacgttgggagtgaccaaacagtcgcaccaggaaaaatgcggctaacgacttaccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGa                                                                                                                  >  1:344518‑M2/116‑150 (MQ=255)                                      gcgccaggctggcggcgatggttaatgccgtacgttgggagtgaccaaacagtcgcaccaggaaaaatgcggctaacgacttaccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTa                                                                                                        >  2:669956‑M2/106‑150 (MQ=255)                                        gccaggctggcggcgatggttaatgccgtacgttgggagtgaccaaacagtcgcaccaggaaaaatgcggctaacgacttaccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAAc                                                                                                      <  2:264629‑M2/47‑1 (MQ=255)                                                    gcgatggttaatgccgtacgttgggagtgaccaaacagtcgcaccaggaaaaatgcggctaacgacttaccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAaacaac                                                                                          <  2:345508‑M2/59‑1 (MQ=255)                                                         ggttaatgccgtacgttgggagtgaccaaacagtcgcaccaggaaaaatgcggctaacgacttaccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATccc                                                                                     >  2:228216‑M2/87‑150 (MQ=255)                                                                  ggTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACATACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGAc                                                                            >  2:428740/1‑150 (MQ=255)                                                                      cgttgggagtgaccaaacagtcgcaccaggaaaaatgcggctaacgacttaccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACaaaa                                                                        >  1:46389‑M2/74‑150 (MQ=255)                                                                      cgttgggagtgaccaaacagtcgcaccaggaaaaatgcggctaacgacttaccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACaaaa                                                                        >  2:452809‑M2/74‑150 (MQ=255)                                                                      cgttgggagtgaccaaacagtcgcaccaggaaaaatgcggctaacgacttaccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACaaaa                                                                        >  2:594068‑M2/74‑150 (MQ=255)                                                                      cgttgggagtgaccaaacagtcgcaccaggaaaaatgcggctaacgacttaccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACaaaa                                                                        >  1:602111‑M2/74‑150 (MQ=255)                                                                              aCGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCTGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCAc                                                                <  2:392836/150‑1 (MQ=255)                                                                               cGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCAcc                                                               >  2:533117/1‑150 (MQ=255)                                                                                      aGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAAcg                                                        <  2:534018/150‑1 (MQ=255)                                                                                                           aggctaacaacttaccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTAt                                   <  1:241891‑M2/114‑1 (MQ=255)                                                                                                             gctaacgacttaccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCg                                 >  2:385974‑M2/35‑150 (MQ=255)                                                                                                             gctaacaacttaccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTAtct                                 >  2:242574‑M2/35‑149 (MQ=255)                                                                                                               taccgacttaccaacaagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTg                               <  1:667984‑M2/118‑1 (MQ=255)                                                                                                                       taccaaacagaataatcgccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCAACGGAGACAGAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGAATCGTGGGCGGCAc                       >  1:413712‑M2/25‑150 (MQ=255)                                                                                                                                         gAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACt     <  1:531576/150‑1 (MQ=255)                                                                                                                                         ccagcgTCTGCAGCGTCGCCAGCACGGCCAGCGGTTGCTGAATCAGAAGTAACGGAGCAAACAACATCCCGACGGAGACTCAAAACAGCGCCGCAAGCGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACt     <  1:618675‑M2/144‑1 (MQ=255)                                                                                                                                         ccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACt     <  2:46531‑M2/144‑1 (MQ=255)                                                                                                                                         ccagcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACt     <  2:241420‑M2/144‑1 (MQ=255)                                                                                                                                            gcgTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGt  >  2:4079‑M2/4‑150 (MQ=255)                                                                                                                                               |                                                                                                                                                   AATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGT  >  REL606/474233‑474521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.