| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| JC JC | REL606 | 502,543 | IS150 (–) +3 bp | 100% | coding (848‑850/1095 nt) | ybbB ← | tRNA 2‑selenouridine synthase, selenophosphate‑dependent |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | 502543 = | 1 (0.040) | 12 (0.450) | 11/278 | NT | 92.2% | coding (850/1095 nt) | ybbB | tRNA 2‑selenouridine synthase, selenophosphate‑dependent |
| ? | REL606 | 588495 = | NA (NA) | noncoding (1/1977 nt) | IS150 | repeat region | |||||
| * | ? | REL606 | = 502545 | 1 (0.040) | 27 (1.010) | 21/278 | NT | 96.4% | coding (848/1095 nt) | ybbB | tRNA 2‑selenouridine synthase, selenophosphate‑dependent |
| ? | REL606 | = 666130 | NA (NA) | noncoding (1443/1443 nt) | IS150 | repeat region | |||||
AATATTCTTCAAGTAACGGCACCAGCCAGGCCAGATGACCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTC > REL606/502396‑502691 | aaTATTCTTCAAGTAACGGCACCAGCCAGGCCAGATGACCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGAt < 2:520485/150‑1 (MQ=255) accaaaacgtgccgagatgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaGATg > 2:631600‑M1/147‑150 (MQ=255) aaccaaaacgtgccgatatgatcctgtaaccatcatcagttgtgaagagtgattcacgactttaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaGATg > 1:99890‑M1/147‑150 (MQ=255) tcttcAAGTAACGGCACCAGCCAGGCCAGATGACCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtac < 2:138071‑M1/150‑6 (MQ=255) gTAACGGCACCAGCCAGGCCAGATGACCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGc < 2:608094/150‑1 (MQ=255) ccgagatgatcctgtaaccataatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggcgttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaGATGCAGGTATTCGc > 1:104843‑M1/136‑150 (MQ=255) atgatcctgtaaccatcatcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaGATGCAGGTATTCGCAATAc > 2:660007‑M1/131‑150 (MQ=255) ccatcatctgttgtgaagtagtgactcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaGATGCAGGTATTCGCAATACTCCTGCCAGccc > 1:465578‑M1/119‑150 (MQ=255) gATGACCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaaca < 1:418149‑M1/150‑34 (MQ=255) ctgACCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCCGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacac < 1:251567‑M1/149‑35 (MQ=255) atcagttgtgaagtagtgattcacgacttcaaggcgcttttcaaaagggtattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTtc > 2:385122‑M1/114‑150 (MQ=255) atcagttgtgaagtagtgattcacgacttcaacgcgcttttcaaaagtggattttggctttgacagattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTtc > 1:456320‑M1/114‑150 (MQ=255) aTGACCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacac < 2:107394‑M1/150‑35 (MQ=255) aTGACCGTCGGTGCTGCCGGTGGGGAGTTGCGTTGTCAGTGCTGCATCCAGTCTTGCAGCCATTTTGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaagagttggacagttaaacac < 1:179027‑M1/150‑35 (MQ=255) gACCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacga > 2:176924‑M1/1‑114 (MQ=255) aCCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgag > 2:485904‑M1/1‑113 (MQ=255) tgcCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtc < 2:15447‑M1/150‑49 (MQ=255) gagacagggtgagttgcgTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtc < 1:571013‑M1/143‑49 (MQ=255) gtggtgAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgatt > 1:50670‑M1/1‑97 (MQ=255) ggtgAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtcctgtctc > 2:572679‑M1/1‑95 (MQ=255) gAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgat < 2:191013‑M1/150‑59 (MQ=255) aaggcgcttttcaaaagggtattttggctttggcatattaggggatattccatttcttcgtccaacaaaatgggtgcagtacaGATGCAGGTATTCGAAATGCTCCAGGCAGCCCTGTTCGTCGCCATACTCGTGGGTAAAGTCatgatg > 1:113225‑M1/84‑150 (MQ=255) gTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtc > 2:519156‑M1/1‑78 (MQ=255) gTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGGAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagatggacagttaaacacgaggcatataggtctgattccgatattcaattggagtc < 1:24433‑M1/150‑73 (MQ=255) tgcATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagac < 2:26227‑M1/150‑77 (MQ=255) tgcATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagac < 1:484527‑M1/150‑77 (MQ=255) aTCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagacctt > 2:437341‑M1/1‑71 (MQ=255) gtattttggcattgacatattaggggctattccatttcatcgtccaacaaaatgggtgaagtacaGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATActc > 1:204‑M1/66‑150 (MQ=255) tattttggctttgacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATActct > 2:642218‑M1/65‑150 (MQ=255) gacatattaggggctattccatttcatcgtccaacaaaatgggtgcagtacaGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTCCGCGTGGGTAAAATCATGATGCATACGCAAGCAATACTCTTCATTCAGgcgc < 1:266904‑M1/98‑1 (MQ=255) attaggggctattccatttcatcgtccaacaaaatgggtgcagtacaGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAg < 2:673979‑M1/103‑1 (MQ=255) ctattccatttcatcgtccaacaaaatgggtgcagtacaGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGAtct < 1:444170‑M1/111‑1 (MQ=255) cTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgt > 2:408201‑M1/1‑41 (MQ=255) cTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagctggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgt > 2:134033‑M1/1‑41 (MQ=255) gTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtag < 2:410407‑M1/150‑112 (MQ=255) aGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagta < 1:436101‑M1/150‑114 (MQ=255) gCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtat < 2:396743‑M1/150‑115 (MQ=255) aGCCGACGCTTAATCGCCGAAAGTCCGTGATtgtactgcccccaaaaagttggacagttaaacccgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaat < 1:109811‑M1/150‑120 (MQ=255) ccGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacactttaacacgggccttatcggtctgattcctatattcaattggggtcagaccttttaatttcaggctcagtcttctgctgttgtagtattctatat < 1:185014‑M1/150‑122 (MQ=255) ccGACGCTTAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagctggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatat < 1:133645‑M1/150‑122 (MQ=255) ttAATCGCCGAAAGTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgt < 1:517670‑M1/150‑129 (MQ=255) agcgccgcaaGTCCGTGATtgtactgacccgaaccagttgagcagttaaacacgactcctataggtctgattccgttattcagttggagtcagaccttttaatttcagcccaattcgtctgctgttgtagtattcaatatattccgtaac < 1:325711‑M1/148‑132 (MQ=255) gtgcagtacaGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATcgcc < 2:49685‑M1/140‑1 (MQ=255) gTCCGTGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttc < 2:331136‑M1/150‑142 (MQ=255) gtacagATGCCGGGATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTCCGCGGGGGTAACATCTTCATGCATACGCACGACATACCCTCCGTCCAGGCGCTCAAGCCGGATCTGAAGCGGATCTTCTACCCCCCCACTCGCCGACTg > 1:525770/6‑150 (MQ=255) gtacaGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTg < 2:644447‑M1/145‑1 (MQ=255) gtacaGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTg < 2:342740‑M1/145‑1 (MQ=255) caGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGt > 2:445432‑M1/3‑150 (MQ=255) tGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcctgtc > 2:458239‑M1/1‑4 (MQ=255) tGATtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttc > 1:240799‑M1/1‑4 (MQ=255) | AATATTCTTCAAGTAACGGCACCAGCCAGGCCAGATGACCGTCGGTGCTGCCGGTGGTGAGTTGCGTTGTCAGTGCTGCATCCAGCCTTGCAGCCAGTTCGTTATAGCGCTGTAGCCCCAGCCGACGCTTAATCGCCGAAAGTCCGTGATGCAGGTATTCGCAATACTCCTGCCAGCCCTGTTCGTCGCCGTACGCGTGGGTAAAATCATGATGCATACGCAAGAAATACTCTTCGTTCAGGCGCTCAAGACGGATCTCAAACGGATCTTCTACCACCGCAATCGCCGCCTGAGTC > REL606/502396‑502691 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |