| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 2,775,878 | Δ1 bp | 100% | intergenic (‑48/+201) | insJ‑3 ← / ← cysH | IS150 hypothetical protein/phosphoadenosine phosphosulfate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,775,878 | 0 | G | . | 100.0% | 110.7 / NA | 26 | intergenic (‑48/+201) | insJ‑3/cysH | IS150 hypothetical protein/phosphoadenosine phosphosulfate reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (10/16); total (10/16) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGCCGAGATGATCCTGTAACCATCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCACCCGACGCCATTCTATACCTGATAATTCTTCCAGCGGCGTTCACGTTTAAAATCAGGAATACTGC > REL606/2775740‑2776018 | gTGCCGAGATGATCCTGTAACCATCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAat > 1:662966/1‑150 (MQ=18) gatgatCCTGTAACCATCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCt > 2:158673/1‑150 (MQ=255) atCCTGTAACCATCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCTCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTgggg < 1:463943/150‑1 (MQ=255) tCCTGTAACCATCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGc > 2:131540/1‑150 (MQ=255) tCCTGTAACCATCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGc > 2:43508/1‑150 (MQ=255) gTAACCATCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCtttt > 1:140504/1‑150 (MQ=255) ataaacataaGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTc < 1:289384/145‑1 (MQ=255) aCCGTCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTc < 1:532769/150‑1 (MQ=255) ccATCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTcc > 1:441846/1‑150 (MQ=255) gTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAAc < 2:140907/150‑1 (MQ=255) gTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAAc < 2:85598/150‑1 (MQ=255) gTTGTGAAGTAGTGATTCACGACTTCAAGGCGCGTTTCAAAAGGGTATTTTGGCTTTGACATATTAGTGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAAc < 1:489075/150‑1 (MQ=255) aCGACTTCAATGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGTGTATTCCATTGCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGGCCATCTGGAACCTCGCGAATGCTTAgcgc < 1:516324/150‑1 (MQ=21) ttttCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGc < 2:664926/150‑1 (MQ=255) cAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCAccc < 2:443096/150‑1 (MQ=255) tGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCACCCGACGCCATTCtat < 1:131161/150‑1 (MQ=255) ataagagacagtatTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCACCCGACGCCATTCtat < 1:334558/139‑1 (MQ=38) gCTTTTACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCACCCGACGCCATTCTATAc < 1:43370/150‑1 (MQ=255) gCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCACCCGACGCCATTCTATAc < 2:51737/150‑1 (MQ=255) gCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCACCCGACGCCATTCTATAc < 1:158228/150‑1 (MQ=255) gACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCACCCGACGCCATTCTATACCTGAt < 2:159733/150‑1 (MQ=255) tatTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCACCCGACGCCATTCtatctgtctcttat > 2:335510/1‑139 (MQ=38) tatTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCACCCGACGCCATTCTATACCTGATAAtt > 2:67183/1‑150 (MQ=255) gggCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCACCCGACGCCATTCTATACCTGATAATTCTTCCa > 2:673664/1‑150 (MQ=255) tGGGTGTAGTAC‑TGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGTAACCTCGCTAATGAGTAACGCCAGACAGCCTCAAGCACCCGACGGCATTTTATACCTGATAATTCTTCCAGCGGCGTTCACGTTTAAAATCAGGAATAc < 1:264980/150‑1 (MQ=255) ggTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCACCCGACGCCATTCTATACCTGATAATTCTTCCAGCGGCGTTCACGTTTAAAATCAGGAATACTg < 1:66985/150‑1 (MQ=255) gTGCAGTACA‑GTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCACCCGACGCCATTCTATACCTGATAATTCTTCCAGCGGCGTTCACGTTTAAAATCAGGAATACTGc > 2:604375/1‑150 (MQ=255) | GTGCCGAGATGATCCTGTAACCATCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTCAAGCACCCGACGCCATTCTATACCTGATAATTCTTCCAGCGGCGTTCACGTTTAAAATCAGGAATACTGC > REL606/2775740‑2776018 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |