| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 1,915,101 | Δ1 bp | 100% | intergenic (‑211/‑127) | zwf ← / → yebK | glucose‑6‑phosphate 1‑dehydrogenase/predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,915,101 | 0 | A | . | 100.0% | 63.8 / NA | 15 | intergenic (‑211/‑127) | zwf/yebK | glucose‑6‑phosphate 1‑dehydrogenase/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (5/10); total (5/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGC > REL606/1914954‑1915237 | gTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATTCACCCGTGAGAGTACGGGAAAAGCGCGCAAATTGCGGCAAAACTGATACAAAAATCATCGTTTTCAATTCGGTACAAATCTGACACTGATCATTTTATGaaa > 1:558860/1‑150 (MQ=255) gTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaaataa > 2:622998/1‑147 (MQ=255) gTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaaataa > 1:519702/1‑147 (MQ=255) gTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaaataa > 2:214169/1‑147 (MQ=255) tACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaaataac < 2:521195/150‑5 (MQ=255) tCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGcacaca < 2:161751/150‑1 (MQ=255) cACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACATGGCACACAgg < 1:621178/150‑1 (MQ=255) cACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAgg < 2:396280/150‑1 (MQ=255) cACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAgg < 2:4889/150‑1 (MQ=255) gCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAata < 1:213555/150‑1 (MQ=255) gCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAata < 2:532451/150‑1 (MQ=255) gCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAata < 1:540366/150‑1 (MQ=255) gCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAata < 1:253060/150‑1 (MQ=255) gCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAata < 1:374161/150‑1 (MQ=255) cGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACa > 1:488673/1‑150 (MQ=255) gTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGAACACAGGCGTAATATCTGACAAAAAGGCATTTCGAttt > 1:606651/1‑150 (MQ=255) cGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCtt > 1:488027/1‑150 (MQ=255) aTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTAcatcat > 2:46189/1‑150 (MQ=255) gacagcTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGaa < 2:627264/145‑1 (MQ=255) cTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGaactgtc > 1:625423/1‑145 (MQ=255) | GTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGC > REL606/1914954‑1915237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |