Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779	1,246,924	2 bp→AA	coding (188‑189/441 nt)	ycgY →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	1,246,924	0	G	A	100.0%	42.9 / NA	11	W63* (TGG→TAA)	ycgY	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/4);  total (7/4)
*	NC_007779	1,246,925	0	G	A	100.0%	42.7 / NA	11	W63* (TGG→TAA)	ycgY	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/4);  total (7/4)

ATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAAT  >  NC_007779/1246843‑1247015                                                                                  ||                                                                                           aTCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTAATTGCTAAATTCTCTGGAg                                                                          <  1:801122/101‑1 (MQ=255)                    ccAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTAATTGCTAAATTCTCTGGAGTGTCGGGAGGATGTGatta                                                       <  4:14516/101‑1 (MQ=255)                              cGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTAATTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAAc                                             >  3:216665/1‑101 (MQ=255)                               gTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTAATTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACt                                            >  2:75351/1‑101 (MQ=255)                                   ggCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTAATTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAg                                        >  3:1529386/1‑101 (MQ=255)                                    gCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTAATTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGa                                       <  3:925253/101‑1 (MQ=255)                                              aGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTAATTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCaaaaaa                             >  2:160272/1‑101 (MQ=255)                                                   cgagAAGAGGAATGGTCTGAATTCTTTTTTTAATTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGtt                        >  4:159575/4‑101 (MQ=255)                                                         gagGAATGGTCTGAATTCTTTTTTTAATTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAg                  >  1:223837/1‑101 (MQ=255)                                                                ggTCTGAATTCTTTTTTTAATTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAacac           >  3:511236/1‑101 (MQ=255)                                                                         tCTTTTTTTAATTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTTATGAGTGAACACCGTAACagt  <  4:106753/101‑3 (MQ=255)                                                                                  ||                                                                                           ATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTCCAGAAAGCAAAAAAACGTTGATGAGTGAACACCGTAACAAT  >  NC_007779/1246843‑1247015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑