Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779	547,694	A→G	intergenic (+123/‑1156)	fdrA → / → ylbF	acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	547,694	0	A	G	100.0%	117.3 / NA	31	intergenic (+123/‑1156)	fdrA/ylbF	acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/15);  total (16/15)

CGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGG  >  NC_007779/547597‑547789                                                                                                  |                                                                                                cgcAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc                                                                                              >  3:1233718/1‑101 (MQ=255)  gcAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCa                                                                                             >  1:178267/1‑101 (MQ=255)  gcAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGACTTCTTCACTTATCAGCGAACTAAACGAGGGCa                                                                                             >  1:430036/1‑101 (MQ=255)        cAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAacac                                                                                       <  3:1304455/101‑1 (MQ=255)          aTGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTg                                                                                     >  3:34489/1‑101 (MQ=255)           tgctgcGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGc                                                                                    <  1:1134538/101‑1 (MQ=255)                     aTCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCgg                                                                          <  3:118127/101‑1 (MQ=255)                      tCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCggg                                                                         >  3:923234/1‑101 (MQ=255)                            aaaTTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCa                                                                   >  3:851097/1‑101 (MQ=255)                                    cgcgCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTg                                                           >  3:399538/1‑101 (MQ=255)                                         tCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAgg                                                      <  1:333368/101‑1 (MQ=255)                                            tCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga                                                   >  3:237373/1‑101 (MQ=255)                                             ccACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt                                                  <  1:329579/101‑1 (MQ=255)                                              cacaCTGGCTGGATGTGCAACCGGCTTCTTCGCTTATCAGCGAACTAACCGGGGACAAAACACTGCTTCACCCCGGGCCGCCAATGCGTTGGCAGGAGATg                                                 <  1:1531092/101‑1 (MQ=255)                                                cacTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGAc                                               <  1:346178/101‑1 (MQ=255)                                                    ggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGa                                           >  1:943690/1‑101 (MQ=255)                                                     gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAc                                          >  3:1311323/1‑101 (MQ=255)                                                     gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAc                                          <  3:1302260/101‑1 (MQ=255)                                                           tgtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa                                    >  3:102920/1‑101 (MQ=255)                                                           tgtgCAACCGCCTTCTTCACTTATCACCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa                                    <  3:637149/101‑1 (MQ=255)                                                                 aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgggg                              <  1:716578/101‑1 (MQ=255)                                                                  ccGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgc                             >  2:162387/1‑101 (MQ=255)                                                                  ccGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgc                             <  3:1446164/101‑1 (MQ=255)                                                                      cttcttCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcgtgc                         <  3:424418/101‑1 (MQ=255)                                                                       ttcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcgtgcg                        >  1:1138948/1‑101 (MQ=255)                                                                        tcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcgtgcgt                       >  1:1510499/1‑101 (MQ=255)                                                                              cTTACCAGCAAACAAAAGGAGGGAAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGgcgc                 <  1:1284388/101‑1 (MQ=255)                                                                                 aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATg              <  1:472625/101‑1 (MQ=255)                                                                                       gAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGtt        <  3:495671/101‑1 (MQ=255)                                                                                         aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCg      >  1:485214/1‑101 (MQ=255)                                                                                             aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAgg  >  3:373973/1‑101 (MQ=255)                                                                                                  |                                                                                                CGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGG  >  NC_007779/547597‑547789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑