Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779	66,528	T→C	Q8R (CAG→CGG)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	66,528	0	T	C	100.0%	162.6 / NA	40	Q8R (CAG→CGG)	araD	L‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/27);  total (13/27)

TCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGC  >  NC_007779/66430‑66624                                                                                                   |                                                                                                 tCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCgg                                                                                                >  1:342529/1‑101 (MQ=255)  cacaAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGc                                                                                               <  1:1203826/101‑1 (MQ=255)  cacaAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGc                                                                                               <  3:337360/101‑1 (MQ=255)        gACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccga                                                                                         >  3:683033‑M1/1‑96 (MQ=255)         aCGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgag                                                                                        >  1:696833‑M1/1‑95 (MQ=255)            ccgcGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacc                                                                                     <  3:1147362‑M1/101‑10 (MQ=255)              gcgcTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccg                                                                                   >  3:58234‑M1/1‑90 (MQ=255)                     cgATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgt                                                                            <  1:827311‑M1/101‑19 (MQ=255)                     cgATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgt                                                                            <  1:81340‑M1/101‑19 (MQ=255)                      gATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtc                                                                           <  1:1022192‑M1/101‑20 (MQ=255)                      gATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtc                                                                           <  1:893269‑M1/101‑20 (MQ=255)                          aaCGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacc                                                                       <  3:79110‑M1/101‑24 (MQ=255)                           aCGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacct                                                                      <  3:1197157‑M1/101‑25 (MQ=255)                            cGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctg                                                                     >  1:97869‑M1/1‑76 (MQ=255)                             ggCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgc                                                                    <  1:1263977‑M1/101‑27 (MQ=255)                             ggCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgc                                                                    <  3:1264204‑M1/101‑27 (MQ=255)                                       ttGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggag                                                          >  3:19566‑M1/1‑65 (MQ=255)                                           cccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaac                                                      <  3:1236473‑M1/101‑41 (MQ=255)                                              aTGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagt                                                   <  3:501450‑M1/101‑44 (MQ=255)                                              aTGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagt                                                   <  1:866331‑M1/101‑44 (MQ=255)                                               tgtgAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagta                                                  <  1:940358‑M1/101‑45 (MQ=255)                                                  gAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagag                                               >  3:751502‑M1/1‑54 (MQ=255)                                                    gCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagag                                             <  3:1492010‑M1/101‑50 (MQ=255)                                                       tGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttg                                          >  1:853185‑M1/1‑49 (MQ=255)                                                            aGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgata                                     <  3:716565‑M1/101‑58 (MQ=255)                                                              gTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaa                                   <  3:1325298‑M1/101‑60 (MQ=255)                                                                tgtgTTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaa                                 >  1:359513‑M1/1‑40 (MQ=255)                                                                   gtTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcg                              >  3:1049216‑M1/1‑37 (MQ=255)                                                                   gtTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcg                              <  3:760702‑M1/101‑65 (MQ=255)                                                                   gtTTTGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcg                              <  1:1038000‑M1/101‑65 (MQ=255)                                                                      ttGGCAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggaggaacagtagagagttgcgataaaaagcgtca                           >  1:626750‑M1/1‑34 (MQ=255)                                                                          cAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggta                       <  1:1027495‑M1/101‑72 (MQ=255)                                                                          cAGCGCCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggta                       <  1:742311‑M1/101‑72 (MQ=255)                                                                             cgcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtagga                    >  1:536308‑M1/1‑27 (MQ=255)                                                                             cgcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtagga                    <  3:1237789‑M1/101‑75 (MQ=255)                                                                             cgcCAGGTTGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggaagga                    >  1:854086‑M1/1‑27 (MQ=255)                                                                                     tGGCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaa            <  3:135384‑M1/101‑83 (MQ=255)                                                                                       gCTTCTAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatc          <  1:37983‑M1/101‑85 (MQ=255)                                                                                            tAATACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatg     <  3:98954‑M1/101‑90 (MQ=255)                                                                                               tACCCGGCGcccgagacccgcacgcgtcgacctgcaggtatggagaaacagtagagagttgcgataaaaagcgtcaggtaggatccgctaatcttatggat  <  3:1599697‑M1/101‑93 (MQ=255)                                                                                                   |                                                                                                 TCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGC  >  NC_007779/66430‑66624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑