Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779	2,842,666	G→T	R67R (CGC→CGA)	hycG ←	hydrogenase 3 and formate hydrogenase complex, HycG subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	2,842,666	0	G	T	100.0%	281.8 / NA	70	R67R (CGC→CGA)	hycG	hydrogenase 3 and formate hydrogenase complex, HycG subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/36);  total (34/36)

ACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACG  >  NC_007779/2842569‑2842761                                                                                                  |                                                                                                acgcacgcAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGt                                                                                              <  1:1207171/101‑1 (MQ=255)    cacgcaGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGTTGAAGGAACGACTTTAATGCCGAATCGTTCt                                                                                           <  1:1049133/101‑1 (MQ=255)     acgcaGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTg                                                                                          <  3:661450/101‑1 (MQ=255)     acgcaGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCAGAATCGTTCTg                                                                                          >  3:1418260/1‑101 (MQ=255)      cgcagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGc                                                                                         >  3:259871/1‑101 (MQ=255)       gcagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCa                                                                                        >  1:1548177/1‑101 (MQ=255)       gcagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCAGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCa                                                                                        >  3:1020700/1‑101 (MQ=255)             cagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAAc                                                                                  >  3:302162/1‑101 (MQ=255)             cagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAAc                                                                                  <  3:936348/101‑1 (MQ=255)             cagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAAc                                                                                  <  1:435975/101‑1 (MQ=255)              agGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACa                                                                                 <  1:1113789/101‑1 (MQ=255)                gggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgc                                                                               <  3:1354628/101‑1 (MQ=255)                gggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgc                                                                               >  3:1344143/1‑101 (MQ=255)                 ggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcg                                                                              <  3:632886/101‑1 (MQ=255)                      gCACTGCACGGGTGACCGCTATGGTAAATAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCAGCGaa                                                                         <  1:1103659/101‑1 (MQ=255)                       cATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGaaa                                                                        >  1:116763/1‑101 (MQ=255)                       cATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGaaa                                                                        >  1:1271600/1‑101 (MQ=255)                        aTTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAg                                                                       >  1:568966/1‑101 (MQ=255)                         ttGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGc                                                                      <  3:1525593/101‑1 (MQ=255)                           gCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGt                                                                    >  1:382821/1‑101 (MQ=255)                            cACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTg                                                                   <  3:20960/101‑1 (MQ=255)                            cACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTg                                                                   <  3:723576/101‑1 (MQ=255)                            cACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTg                                                                   <  1:966950/101‑1 (MQ=255)                                  tGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaa                                                             >  1:1455464/1‑101 (MQ=255)                                  tGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaa                                                             >  1:75488/1‑101 (MQ=255)                                  tGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaa                                                             >  1:1347627/1‑101 (MQ=255)                                   gACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaaa                                                            >  1:957543/1‑101 (MQ=255)                                     ccGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatt                                                          >  3:127970/1‑101 (MQ=255)                                      cgcgCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAattt                                                         <  3:1255575/101‑1 (MQ=255)                                       gcgcCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttc                                                        <  2:134798/101‑1 (MQ=255)                                                 aCAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCaa                                              >  3:1008235/1‑101 (MQ=255)                                                 aCAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCaa                                              >  3:87005/1‑101 (MQ=255)                                                  cAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAc                                             >  3:576712/1‑101 (MQ=255)                                                  cAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAc                                             >  1:198760/1‑101 (MQ=255)                                                       aaaTATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTg                                        <  1:774002/101‑1 (MQ=255)                                                         atatCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGcg                                      >  1:1316057/1‑100 (MQ=255)                                                         atatCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCa                                      >  3:1584337/1‑101 (MQ=255)                                                          tatCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCAGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAg                                     >  3:242301/1‑101 (MQ=255)                                                            tCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGATAATTTCGATTTCGCAACCGTTGCAgcc                                   >  1:1086712/1‑101 (MQ=255)                                                            tCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgcc                                   <  3:1204961/101‑1 (MQ=255)                                                            tCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgcc                                   >  1:683617/1‑101 (MQ=255)                                                            tCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgcc                                   <  1:512260/101‑1 (MQ=255)                                                             ccGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccg                                  <  1:1294745/101‑1 (MQ=255)                                                              cGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccgc                                 >  2:117400/1‑101 (MQ=255)                                                              cGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccgc                                 <  1:865478/101‑1 (MQ=255)                                                                cATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGccgccg                               >  1:308891/1‑101 (MQ=255)                                                                   gACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAg                            <  3:1564201/101‑1 (MQ=255)                                                                     cgcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTc                          <  3:296056/101‑1 (MQ=255)                                                                     cgcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTc                          <  3:1271935/101‑1 (MQ=255)                                                                      gcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTcc                         >  3:116892/1‑101 (MQ=255)                                                                      gcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTcc                         >  3:426840/1‑101 (MQ=255)                                                                      gcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTcc                         <  1:978059/101‑1 (MQ=255)                                                                        ggTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAc                       >  3:210562/1‑101 (MQ=255)                                                                         gTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcg                      >  1:740924/1‑101 (MQ=255)                                                                         gTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcg                      <  1:570443/101‑1 (MQ=255)                                                                           gAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcgcg                    <  3:588617/101‑1 (MQ=255)                                                                             aGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGt                  >  1:1265611/1‑101 (MQ=255)                                                                                aaCGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTaaa               >  1:390142/1‑101 (MQ=255)                                                                                  cGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACa             <  3:275133/101‑1 (MQ=255)                                                                                  cGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACa             >  3:195187/1‑101 (MQ=255)                                                                                  cGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACa             <  3:1410865/101‑1 (MQ=255)                                                                                    aCTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATa           <  1:967032/101‑1 (MQ=255)                                                                                    aCTTTAATGCCGAATAGTTCTTCATCAAACAGCGACGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATa           <  1:1010969/101‑1 (MQ=38)                                                                                      tttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAgg         <  3:1462409/101‑1 (MQ=255)                                                                                         aaTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAg      <  3:76375/101‑1 (MQ=255)                                                                                         aaTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAg      >  3:1458828/1‑101 (MQ=255)                                                                                          aTGCCTAATCGTTCTTCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGa     <  1:1404115/101‑1 (MQ=255)                                                                                          aTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGa     <  1:258462/101‑1 (MQ=255)                                                                                            gCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGTTTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAAc   <  3:393181/101‑1 (MQ=255)                                                                                             ccGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACg  <  3:231008/101‑1 (MQ=255)                                                                                                  |                                                                                                ACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACG  >  NC_007779/2842569‑2842761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑