Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007779 2,842,666 G→T R67R (CGC→CGA hycG ← hydrogenase 3 and formate hydrogenase complex, HycG subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077792,842,6660GT100.0% 281.8 / NA 70R67R (CGC→CGAhycGhydrogenase 3 and formate hydrogenase complex, HycG subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/36);  total (34/36)

ACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACG  >  NC_007779/2842569‑2842761
                                                                                                 |                                                                                               
acgcacgcAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGt                                                                                              <  1:1207171/101‑1 (MQ=255)
   cacgcaGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGTTGAAGGAACGACTTTAATGCCGAATCGTTCt                                                                                           <  1:1049133/101‑1 (MQ=255)
    acgcaGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTg                                                                                          <  3:661450/101‑1 (MQ=255)
    acgcaGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCAGAATCGTTCTg                                                                                          >  3:1418260/1‑101 (MQ=255)
     cgcagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGc                                                                                         >  3:259871/1‑101 (MQ=255)
      gcagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCa                                                                                        >  1:1548177/1‑101 (MQ=255)
      gcagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCAGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCa                                                                                        >  3:1020700/1‑101 (MQ=255)
            cagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAAc                                                                                  >  3:302162/1‑101 (MQ=255)
            cagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAAc                                                                                  <  3:936348/101‑1 (MQ=255)
            cagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAAc                                                                                  <  1:435975/101‑1 (MQ=255)
             agGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACa                                                                                 <  1:1113789/101‑1 (MQ=255)
               gggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgc                                                                               <  3:1354628/101‑1 (MQ=255)
               gggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgc                                                                               >  3:1344143/1‑101 (MQ=255)
                ggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcg                                                                              <  3:632886/101‑1 (MQ=255)
                     gCACTGCACGGGTGACCGCTATGGTAAATAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCAGCGaa                                                                         <  1:1103659/101‑1 (MQ=255)
                      cATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGaaa                                                                        >  1:116763/1‑101 (MQ=255)
                      cATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGaaa                                                                        >  1:1271600/1‑101 (MQ=255)
                       aTTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAg                                                                       >  1:568966/1‑101 (MQ=255)
                        ttGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGc                                                                      <  3:1525593/101‑1 (MQ=255)
                          gCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGt                                                                    >  1:382821/1‑101 (MQ=255)
                           cACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTg                                                                   <  3:20960/101‑1 (MQ=255)
                           cACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTg                                                                   <  3:723576/101‑1 (MQ=255)
                           cACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTg                                                                   <  1:966950/101‑1 (MQ=255)
                                 tGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaa                                                             >  1:1455464/1‑101 (MQ=255)
                                 tGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaa                                                             >  1:75488/1‑101 (MQ=255)
                                 tGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaa                                                             >  1:1347627/1‑101 (MQ=255)
                                  gACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaaa                                                            >  1:957543/1‑101 (MQ=255)
                                    ccGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatt                                                          >  3:127970/1‑101 (MQ=255)
                                     cgcgCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAattt                                                         <  3:1255575/101‑1 (MQ=255)
                                      gcgcCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttc                                                        <  2:134798/101‑1 (MQ=255)
                                                aCAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCaa                                              >  3:1008235/1‑101 (MQ=255)
                                                aCAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCaa                                              >  3:87005/1‑101 (MQ=255)
                                                 cAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAc                                             >  3:576712/1‑101 (MQ=255)
                                                 cAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAc                                             >  1:198760/1‑101 (MQ=255)
                                                      aaaTATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTg                                        <  1:774002/101‑1 (MQ=255)
                                                        atatCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGcg                                      >  1:1316057/1‑100 (MQ=255)
                                                        atatCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCa                                      >  3:1584337/1‑101 (MQ=255)
                                                         tatCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCAGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAg                                     >  3:242301/1‑101 (MQ=255)
                                                           tCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGATAATTTCGATTTCGCAACCGTTGCAgcc                                   >  1:1086712/1‑101 (MQ=255)
                                                           tCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgcc                                   <  3:1204961/101‑1 (MQ=255)
                                                           tCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgcc                                   >  1:683617/1‑101 (MQ=255)
                                                           tCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgcc                                   <  1:512260/101‑1 (MQ=255)
                                                            ccGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccg                                  <  1:1294745/101‑1 (MQ=255)
                                                             cGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccgc                                 >  2:117400/1‑101 (MQ=255)
                                                             cGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccgc                                 <  1:865478/101‑1 (MQ=255)
                                                               cATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGccgccg                               >  1:308891/1‑101 (MQ=255)
                                                                  gACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAg                            <  3:1564201/101‑1 (MQ=255)
                                                                    cgcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTc                          <  3:296056/101‑1 (MQ=255)
                                                                    cgcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTc                          <  3:1271935/101‑1 (MQ=255)
                                                                     gcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTcc                         >  3:116892/1‑101 (MQ=255)
                                                                     gcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTcc                         >  3:426840/1‑101 (MQ=255)
                                                                     gcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTcc                         <  1:978059/101‑1 (MQ=255)
                                                                       ggTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAc                       >  3:210562/1‑101 (MQ=255)
                                                                        gTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcg                      >  1:740924/1‑101 (MQ=255)
                                                                        gTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcg                      <  1:570443/101‑1 (MQ=255)
                                                                          gAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcgcg                    <  3:588617/101‑1 (MQ=255)
                                                                            aGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGt                  >  1:1265611/1‑101 (MQ=255)
                                                                               aaCGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTaaa               >  1:390142/1‑101 (MQ=255)
                                                                                 cGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACa             <  3:275133/101‑1 (MQ=255)
                                                                                 cGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACa             >  3:195187/1‑101 (MQ=255)
                                                                                 cGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACa             <  3:1410865/101‑1 (MQ=255)
                                                                                   aCTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATa           <  1:967032/101‑1 (MQ=255)
                                                                                   aCTTTAATGCCGAATAGTTCTTCATCAAACAGCGACGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATa           <  1:1010969/101‑1 (MQ=38)
                                                                                     tttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAgg         <  3:1462409/101‑1 (MQ=255)
                                                                                        aaTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAg      <  3:76375/101‑1 (MQ=255)
                                                                                        aaTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAg      >  3:1458828/1‑101 (MQ=255)
                                                                                         aTGCCTAATCGTTCTTCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGa     <  1:1404115/101‑1 (MQ=255)
                                                                                         aTGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGa     <  1:258462/101‑1 (MQ=255)
                                                                                           gCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGTTTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAAc   <  3:393181/101‑1 (MQ=255)
                                                                                            ccGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACg  <  3:231008/101‑1 (MQ=255)
                                                                                                 |                                                                                               
ACGCACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGAACG  >  NC_007779/2842569‑2842761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: