Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779	64,108	G→T	S558* (TCG→TAG)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	64,108	0	G	T	100.0%	234.2 / NA	57	S558* (TCG→TAG)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/29);  total (28/29)

TAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCGAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGAT  >  NC_007779/64011‑64205                                                                                                  |                                                                                                  tAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAAt                                                                                                >  3:428927/1‑101 (MQ=255) tAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAAt                                                                                                >  3:1089676/1‑101 (MQ=255)   aTGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAAtgt                                                                                              >  2:70104/1‑101 (MQ=255)    tGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAAtgtg                                                                                             >  3:824880/1‑101 (MQ=255)       gcTGGTCAGTCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTgcgc                                                                                          <  3:1330300/101‑1 (MQ=255)       gcTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCTCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTgcgc                                                                                          <  3:1100641/101‑1 (MQ=255)        cTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGcc                                                                                         >  1:1547578/1‑101 (MQ=255)        cTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGcc                                                                                         >  3:452571/1‑101 (MQ=255)                  gttgttTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAg                                                                               >  3:46552/1‑101 (MQ=255)                   ttgttTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGa                                                                              <  3:674044/101‑1 (MQ=255)                   ttgttTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGa                                                                              <  3:512479/101‑1 (MQ=255)                    tgttTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGAc                                                                             >  1:1147169/1‑101 (MQ=255)                         ttGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACg                                                                        >  3:596467/1‑101 (MQ=255)                           gCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTGAGCCAGACAAACGtt                                                                      >  1:1578164/1‑101 (MQ=255)                             aGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGa                                                                    >  3:1079045/1‑101 (MQ=255)                             aGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGa                                                                    <  3:1279249/101‑1 (MQ=255)                              gCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAg                                                                   <  1:833552/101‑1 (MQ=255)                                 tttCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCg                                                                <  1:1303441/101‑1 (MQ=255)                                 tttCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCg                                                                >  3:478987/1‑101 (MQ=255)                                  ttCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCgg                                                               >  1:980656/1‑101 (MQ=255)                                  ttCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCgg                                                               >  1:281472/1‑101 (MQ=255)                                      gCCCCCCAGGCGTTAACGTGCTGCACCAGTGCTGGACCGATTTTCGCCCCTTCTTCTTCCTAATGTGCGCTTTTCAGCCAGACAAACGTTGAGTCGGTATc                                                           <  1:1049192/101‑1 (MQ=255)                                      gCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATc                                                           >  3:1314263/1‑101 (MQ=255)                                      gCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATc                                                           <  3:51431/101‑1 (MQ=255)                                      gCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATc                                                           >  3:650390/1‑101 (MQ=255)                                       cccACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCg                                                          <  3:1034/101‑1 (MQ=255)                                                cGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATa                                                 >  1:995850/1‑101 (MQ=255)                                                cGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATa                                                 >  1:678295/1‑101 (MQ=255)                                                 gTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATaa                                                <  3:743638/101‑1 (MQ=255)                                                  ttAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAAc                                               <  1:813303/101‑1 (MQ=255)                                                   tAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAAcg                                              <  3:1573807/101‑1 (MQ=255)                                                     aCGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAAcgtc                                            >  3:1340932/1‑101 (MQ=255)                                                     aCGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAAcgtc                                            <  1:1446167/101‑1 (MQ=255)                                                      cGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAAcgtcg                                           >  1:805408/1‑101 (MQ=255)                                                        tgctgcACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTa                                         <  1:455675/101‑1 (MQ=255)                                                        tgctgcACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTa                                         <  1:85955/101‑1 (MQ=255)                                                         gctgcACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAg                                        >  3:93045/1‑101 (MQ=255)                                                         gctgcACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAg                                        <  1:501963/101‑1 (MQ=255)                                                            gcACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGccc                                     >  1:339508/1‑101 (MQ=255)                                                             cACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCt                                    <  3:1383855/101‑1 (MQ=255)                                                             cACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCt                                    <  3:378810/101‑1 (MQ=255)                                                             cACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCt                                    <  1:584223/101‑1 (MQ=255)                                                             cACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCt                                    >  1:461829/1‑101 (MQ=255)                                                               ccAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCtgt                                  <  3:736931/101‑1 (MQ=255)                                                                cAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCtgtg                                 <  3:898000/101‑1 (MQ=255)                                                                  gTGCACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCt                               >  1:200319/1‑101 (MQ=255)                                                                     cACGACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTtca                            <  1:754460/101‑1 (MQ=255)                                                                        gACCGATTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTtcaatc                         <  3:33820/101‑1 (MQ=255)                                                                             aTTTTCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGGTTCAATCAACGc                    >  1:1497096/1‑101 (MQ=255)                                                                                 tCGCCGCTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTg                >  3:548599/1‑101 (MQ=255)                                                                                      gcTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCTGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTg           <  1:425445/101‑1 (MQ=255)                                                                                      gcTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTg           >  3:42300/1‑101 (MQ=255)                                                                                      gcTTCTTCTTCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTg           <  1:1079496/101‑1 (MQ=255)                                                                                       cttcttcttCCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGc          <  1:1141158/101‑1 (MQ=255)                                                                                        ttcttcttcCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGcc         >  1:564941/1‑101 (MQ=255)                                                                                         tcttcttcCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCg        <  2:82226/101‑1 (MQ=255)                                                                                               tcCTAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGAt  <  3:121907/101‑1 (MQ=255)                                                                                                  |                                                                                                  TAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGATTTTCGCCGCTTCTTCTTCCGAATGTGCGCCTTTCAGCCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGAT  >  NC_007779/64011‑64205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑