Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779	1,778,780	G→A	D494N (GAT→AAT)	ydiF →	fused acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit and beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	1,778,780	0	G	A	100.0%	51.3 / NA	14	D494N (GAT→AAT)	ydiF	fused acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit and beta subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/5);  total (9/5)

TGGATGTTCGTTATATCACTGAGCGCGCAGTATTCACGCTGAAAGAAGACGGCCTGCATTTAATCGAAATCGCCCCTGGCGTCGATTTACAAAAAGATATTCTCGACAAAATGGATTTCACCCCAGTGATTTCGCCAGAACTCAAACTGATGGACGAAAGATTATTTATCGATGCGGCGATGGGTTTTGT  >  NC_007779/1778685‑1778874                                                                                                |                                                                                               tGGATGTTCGTTATATCACTGAGCGCGCAGTATTCACGCTGAAAGAAGACGGCCTGCATTTAATCGAAATCGCACCTGGCGTCGATTTACAAAAAAATAtt                                                                                           >  3:502244/1‑101 (MQ=255)        tCGTTATATCACTGAGCGCGCAGTATTCACGCTGAAAGAAGACGGCCTGCATTTAATCGAAATCGCCCCTGGCGTCGATTTACAAAAAAATATTCTCGACa                                                                                    <  1:142884/101‑1 (MQ=255)        tCGTTATATCACTGAGCGCGCAGTATTCACGCTGAAAGAAGACGGCCTGCATTTAATCGAAATCGCCCCTGGCGTCCATTTACAAAAAAATATTCTCGGCa                                                                                    <  1:1441782/101‑1 (MQ=255)                   cTGAGCGCGCAGTATTCACGCTGAAAGAAGACGGCCTGCATTTAATCGAAATCGCCCCTGGCGTCGATTTACAAAAAAATATTCTCGACAAAATGGATTTc                                                                         >  1:444571/1‑101 (MQ=255)                    tGAGCGCGCAGTATTCACGCTGAAAGAAGACGGCCTGCATTTAATCGAAATCGCCCCTGGCGTCGATTTACAAAAAAATATTCTCGACAAAATGGATTTCa                                                                        >  1:268013/1‑101 (MQ=255)                                       cTGAAAGAAGACGGCCTGCATTTAATCGAAATCGCCCCTGGCGTCGATTTACAAACAAATCTTCTCGACAACATGGATTTCACCCCAGTGATTTCGTCAGa                                                     >  1:1586134/1‑101 (MQ=255)                                            agaagaCGGCCTGCATTTAATCGAAATCGCCCCTGGCGTCGATTTACAAAAAAATATTCTCGACAAAATGGATTTCACCCCAGTGATTTCGCCAGAACTCa                                                <  1:371173/101‑1 (MQ=255)                                                gaCGGCCTGCATTTAATCGAAATCGCCCCTGGCGTCGATTTACAAAAAAATATTCTCGACAAAATGGATTTCACCCCAGTGATTTCGCCAGAACTCAAACt                                            <  1:497787/101‑1 (MQ=255)                                                                tCGAAATCGCCCCTGGCGTCGATTTACAAAAAAATATTCTCGACAAAATGGATTTCACCCCAGTGATTTCGCCAGAACTCAAACTGATGGACGAAAGatta                            >  1:1515937/1‑101 (MQ=255)                                                                   aaaTCGCCCCTGGCGTCGATTTACAAAAAAATATTCTCGACAAAATGGATTTCACCCCAGTGATTTCGCCAGAACTCAAACTGATGGACGAAAGAttattt                         >  1:363479/1‑101 (MQ=255)                                                                                    gATTTACACACAACTATTCTCGACAAAATGGATTTCACCCCAGTGATTTCGCCAGAACTCAAACTGATGGACGAAAGATTATTTATCGATGCGGCGATggg        >  4:62530/1‑101 (MQ=255)                                                                                    gATTTACAAAAAAATATTCTCGACAAAATGGATTTCACCCCAGTGATTTCGCCAGAACTCAAACTGATGGACGAAAGATTATTTATCGATGCGGCGATggg        >  3:190405/1‑101 (MQ=255)                                                                                     aTTTACAAAAAAATATTCTCGACAAAATGGATTTCACCCCAGTGATTTCGCCAGAACTCAAACTGATGGACGAAAGATTATTTATCGATGCGGCGATGGGt       <  3:448325/101‑1 (MQ=255)                                                                                          cAAAAAAATATTCTCGACAAAATGGATTTCACCCCAGTGATTTCGCCAGAACTCAAACTGATGGACGAAAGATTATTTATCGATGCGGCGATGGGTTTTGt  >  1:1564229/1‑101 (MQ=255)                                                                                                |                                                                                               TGGATGTTCGTTATATCACTGAGCGCGCAGTATTCACGCTGAAAGAAGACGGCCTGCATTTAATCGAAATCGCCCCTGGCGTCGATTTACAAAAAGATATTCTCGACAAAATGGATTTCACCCCAGTGATTTCGCCAGAACTCAAACTGATGGACGAAAGATTATTTATCGATGCGGCGATGGGTTTTGT  >  NC_007779/1778685‑1778874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑