Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779	1,452,268	G→A	L266L (CTG→TTG)	tynA ←	tyramine oxidase, copper‑requiring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	1,452,268	0	G	A	100.0%	213.1 / NA	50	L266L (CTG→TTG)	tynA	tyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/24);  total (26/24)

AACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATT  >  NC_007779/1452170‑1452363                                                                                                   |                                                                                                aaCGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGc                                                                                               >  3:948736/1‑101 (MQ=255)    gACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCAt                                                                                            >  3:352156/1‑101 (MQ=255)       cGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCtt                                                                                         <  3:38145/101‑1 (MQ=255)          caccaGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTAc                                                                                      >  3:940307/1‑101 (MQ=255)           accaGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTAcc                                                                                     >  1:687375/1‑101 (MQ=255)              aGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATc                                                                                  <  1:27090/101‑1 (MQ=255)                 ttttCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGaa                                                                               <  3:1169963/101‑1 (MQ=255)                  tttCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGaaa                                                                              >  1:735380/1‑101 (MQ=255)                     cGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATaa                                                                           <  3:440636/101‑1 (MQ=255)                      gATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAAc                                                                          >  3:178390/1‑101 (MQ=255)                      gATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAAc                                                                          >  3:336997/1‑101 (MQ=255)                      gATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTGTCAAGCCATCTTTACCATCGAAATAAc                                                                          >  1:1082676/1‑101 (MQ=255)                       aTGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAAcc                                                                         >  1:1376459/1‑101 (MQ=255)                        tGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCt                                                                        >  1:1489797/1‑101 (MQ=255)                         gggATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTa                                                                       >  1:424847/1‑101 (MQ=255)                         gggATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTa                                                                       >  1:745600/1‑101 (MQ=255)                           gATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACg                                                                     <  3:889845/101‑1 (MQ=255)                           gATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACg                                                                     <  3:111823/101‑1 (MQ=255)                                 cccAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAgc                                                               <  3:792161/101‑1 (MQ=255)                                     gtagtTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAgcggcg                                                           >  3:1273829/1‑101 (MQ=255)                                         ttGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACTGTCAGCGGCGTGGt                                                       <  4:53854/101‑1 (MQ=255)                                         ttGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGt                                                       <  3:1092892/101‑1 (MQ=255)                                          tGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTa                                                      <  3:290567/101‑1 (MQ=255)                                             cATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATc                                                   >  1:1263467/1‑101 (MQ=255)                                                cACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCGACGGTCAGCGGCGTGGTAATCACt                                                <  4:107175/101‑1 (MQ=255)                                                   cGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACtttt                                             >  4:109312/1‑101 (MQ=255)                                                     aCATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACtttttt                                           <  1:193996/101‑1 (MQ=255)                                                      cATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTc                                          <  3:408150/101‑1 (MQ=255)                                                        tCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGc                                        >  1:404967/1‑101 (MQ=255)                                                          aaGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCAt                                      <  3:385386/101‑1 (MQ=255)                                                            gATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCa                                    <  1:1019727/101‑1 (MQ=255)                                                            gATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCa                                    <  3:872877/101‑1 (MQ=255)                                                            gATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCa                                    >  3:899397/1‑101 (MQ=255)                                                              tAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGt                                  <  3:454006/101‑1 (MQ=255)                                                                 cTGATGACTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAAt                               >  3:1065503/1‑101 (MQ=255)                                                                  tgatgaCTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATa                              >  1:832835/1‑101 (MQ=255)                                                                    atgaCTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATAcc                            <  3:372723/101‑1 (MQ=255)                                                                     tgaCTTTGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACcg                           >  2:89891/1‑101 (MQ=255)                                                                         tttGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGtt                       >  3:1288221/1‑101 (MQ=255)                                                                           tGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTttc                     <  1:939347/101‑1 (MQ=255)                                                                           tGAGCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTttc                     <  1:177024/101‑1 (MQ=255)                                                                              gCAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTttcttc                  <  3:871607/101‑1 (MQ=255)                                                                               cAACCGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCa                 >  3:1443793/1‑101 (MQ=255)                                                                                  ccGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACgg              >  3:1002229/1‑101 (MQ=255)                                                                                   cGGGCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGc             >  1:1174007/1‑101 (MQ=255)                                                                                    gggCATCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCa            <  1:1383414/101‑1 (MQ=255)                                                                                       cATCTTGTTTCAAGCCATCTTTACCATCGAAATACCCTACGGTCAGCGGCGTGGTACTCACTTTTTTCGCATCAGTAATACCACGTCTCTTCACGGAAGCg         >  3:1515893/1‑101 (MQ=255)                                                                                        aTCTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCgg        >  1:917308/1‑101 (MQ=255)                                                                                          cTTGTTTCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCa      <  3:1478502/101‑1 (MQ=255)                                                                                              tttCAAGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAAtt  <  3:1390277/101‑1 (MQ=255)                                                                                                   |                                                                                                AACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATT  >  NC_007779/1452170‑1452363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑