Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779	1,145,280	A→G	L222S (TTA→TCA)	rne ←	fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	1,145,280	0	A	G	100.0%	90.8 / NA	23	L222S (TTA→TCA)	rne	fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/17);  total (6/17)

GCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGATGGCTTCCCAG  >  NC_007779/1145183‑1145375                                                                                                  |                                                                                                gcccatgtaaTCCGGGCGACCTATTGCAGCGATGTCCTGGCGTGCCAGTGCGAGCACTTTCGGGTTATCGATGATGTTTTCGCCGATGTCCTGGCgtgagt                                                                                              <  1:637116/93‑1 (MQ=255)  ctgctgAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtgagta                                                                                             <  1:1454277/101‑1 (MQ=255)   tgctgAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtgagtaa                                                                                            <  3:1531156/101‑1 (MQ=255)    gctgAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAAt                                                                                           >  1:1213572/1‑101 (MQ=255)     ctgAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATc                                                                                          <  3:1301338/101‑1 (MQ=255)       gAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATcgc                                                                                        <  3:119937/101‑1 (MQ=255)        aaaTCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATcgcg                                                                                       <  3:904163/101‑1 (MQ=255)             cGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATg                                                                                  >  1:1218721/1‑101 (MQ=255)                    ccTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAAc                                                                           <  3:1247446/101‑1 (MQ=255)                      tAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGa                                                                         >  3:1522881/1‑101 (MQ=255)                          gCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTAc                                                                     <  3:256453/101‑1 (MQ=255)                                 tatGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGctc                                                              <  1:1227122/101‑1 (MQ=255)                                          gTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCtgatga                                                     <  3:387602/101‑1 (MQ=255)                                                gTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGTGCGAACGATTACGTTGCTCTCCTGATGAATCAgg                                               <  1:1342972/101‑1 (MQ=255)                                                 ttCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGa                                              <  3:1358892/101‑1 (MQ=255)                                                  tCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGaa                                             <  3:332127/101‑1 (MQ=255)                                                    gAGTACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACg                                           <  1:580349/101‑1 (MQ=255)                                                    gAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACg                                           <  3:1285610/101‑1 (MQ=255)                                                     aGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACgg                                          <  3:1022075/101‑1 (MQ=255)                                                      gCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACggg                                         >  1:1200727/1‑101 (MQ=255)                                                                  tATCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCtt                             <  1:1101552/101‑1 (MQ=255)                                                                   aTCGATAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCttt                            <  3:678842/101‑1 (MQ=255)                                                                        tAAGGATTTCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCg                       <  3:573359/101‑1 (MQ=255)                                                                              tttCGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCtttt                 >  1:224361/1‑101 (MQ=255)                                                                                 cGCCGATGTCCTGACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTg              >  1:193788/1‑101 (MQ=255)                                                                                             gACGTGAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGATGGCTTCCCAg  <  2:149341/101‑1 (MQ=255)                                                                                                  |                                                                                                GCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGATGGCTTCCCAG  >  NC_007779/1145183‑1145375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑