Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779	705,435	T→C	L357S (TTG→TCG)	nagE →	fused N‑acetyl glucosamine‑specific PTS enzyme IICBA components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	705,435	0	T	C	100.0%	138.8 / NA	34	L357S (TTG→TCG)	nagE	fused N‑acetyl glucosamine‑specific PTS enzyme IICBA components
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/11);  total (23/11)

CGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTC  >  NC_007779/705337‑705530                                                                                                   |                                                                                                cgcgTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCgg                                                                                               >  1:6735/1‑101 (MQ=255)   cgTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                             >  3:220678/1‑101 (MQ=255)        aTGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCg                                                                                        <  3:11321/101‑1 (MQ=255)           tataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAt                                                                                     <  3:1018716/101‑1 (MQ=255)              aaCCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGGTGGGCGTTATCTTTTTCGGTATCTACTCCGAGGTGTTCAGTTCGGATATCCGCATGtt                                                                                  >  1:861205/1‑101 (MQ=255)                   gccggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTTTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAcc                                                                             <  1:68352/101‑1 (MQ=255)                        ccgccAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaa                                                                        <  2:120211/101‑1 (MQ=255)                        ccgccAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaa                                                                        <  2:91781/101‑1 (MQ=255)                          gccagccaGAACGTCTGGATGCTGCTGGTGGTGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAc                                                                      <  2:63088/101‑1 (MQ=255)                          gccagccaGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAc                                                                      >  3:1234604/1‑101 (MQ=255)                          gccagccaGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAc                                                                      <  2:63490/101‑1 (MQ=255)                          gccagccaGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGCGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAc                                                                      >  1:772539/1‑101 (MQ=255)                             agccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGcc                                                                   >  3:712508/1‑101 (MQ=255)                              gccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCg                                                                  >  3:71482/1‑101 (MQ=255)                                      gTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGa                                                          <  1:447500/101‑1 (MQ=255)                                              gctgctGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaag                                                  >  3:1300477/1‑101 (MQ=255)                                               ctgctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaaga                                                 >  1:1559161/1‑101 (MQ=255)                                                   tgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGAc                                             >  1:1007628/1‑101 (MQ=255)                                                              gTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTAc                                  >  1:938980/1‑101 (MQ=255)                                                                tATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTg                                <  2:157362/101‑1 (MQ=255)                                                                          gCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCaa                      >  1:192613/1‑101 (MQ=255)                                                                            tATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACa                    >  2:63824/1‑101 (MQ=255)                                                                            tATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACa                    >  1:841378/1‑101 (MQ=255)                                                                             aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAg                   <  3:1567205/101‑1 (MQ=255)                                                                             aTCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAg                   >  2:141733/1‑101 (MQ=255)                                                                                tACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCaa                >  3:922836/1‑101 (MQ=255)                                                                                tACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCaa                >  3:349254/1‑101 (MQ=255)                                                                                  cTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAaca              >  3:1488088/1‑101 (MQ=255)                                                                                   ttCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGATACTGAAGAAGCCAACAGCAacac             >  1:1568650/1‑101 (MQ=255)                                                                                    tCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACt            <  1:1019181/101‑1 (MQ=255)                                                                                       tggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTgaa         >  4:150726/1‑101 (MQ=255)                                                                                        ggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTgaag        >  4:96701/1‑101 (MQ=255)                                                                                         gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTgaagg       >  1:1511265/1‑100 (MQ=255)                                                                                              cAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTc  >  1:598618/1‑101 (MQ=255)                                                                                                   |                                                                                                CGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTC  >  NC_007779/705337‑705530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑