Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007779 649,229 A→T L162H (CTC→CAC)  citE ← citrate lyase, citryl‑ACP lyase (beta) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007779649,2290AT100.0% 105.6 / NA 27L162H (CTC→CAC) citEcitrate lyase, citryl‑ACP lyase (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (10/17);  total (10/17)

GCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCCAGCAGGCCGGTGC  >  NC_007779/649136‑649322
                                                                                             |                                                                                             
gcgGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGAt                                                                                        <  3:1178013/101‑1 (MQ=255)
     cTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGa                                                                                   >  3:1368466/1‑101 (MQ=255)
           aaTGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAAc                                                                             <  4:28262/101‑1 (MQ=255)
                   aGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGaa                                                                     <  3:281965/101‑1 (MQ=255)
                           aaCAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCCCCGTGGGCGATACCGCTCAAACGCTCGGAAGCGTGAGc                                                             <  1:1578941/101‑1 (MQ=255)
                            aCAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCg                                                            <  3:949092/101‑1 (MQ=255)
                              agcagTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGAt                                                          >  3:455104/1‑101 (MQ=255)
                                  gttcagttcCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTcc                                                      <  3:411843/101‑1 (MQ=255)
                                   ttcagttcCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCa                                                     >  3:861639/1‑101 (MQ=255)
                                           cttccGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACAGATCAATCGCTCGGTAGCGTGAGCGATTTCCACTGCGCgg                                             <  3:1552102/101‑1 (MQ=255)
                                                  ggAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATcc                                      <  3:1107414/101‑1 (MQ=255)
                                                      cGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAg                                  >  1:251462/1‑101 (MQ=255)
                                                         tCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCgg                               >  3:359719/1‑101 (MQ=255)
                                                          cTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGga                              <  1:884454/101‑1 (MQ=255)
                                                            gTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGgaga                            <  3:971930/101‑1 (MQ=255)
                                                            gTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGgaga                            >  3:214699/1‑101 (MQ=255)
                                                            gTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGgaga                            <  3:719398/101‑1 (MQ=255)
                                                                     ttGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATcgc                   <  1:541337/101‑1 (MQ=255)
                                                                       gcgcACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATcgccg                 >  3:787382/1‑101 (MQ=255)
                                                                        cgcACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATcgccgc                <  1:81135/101‑1 (MQ=255)
                                                                          cacaTAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCca              <  1:821190/101‑1 (MQ=255)
                                                                            caTAGTCTTCCGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCCCcacc            >  1:1584802/1‑99 (MQ=255)
                                                                             aTAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCcagca           <  1:357094/101‑1 (MQ=255)
                                                                                   ttctGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCCAGCAGGCCgg     <  1:1354888/101‑1 (MQ=255)
                                                                                    tctGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCCAGCAGGCCGGt    >  3:937195/1‑101 (MQ=255)
                                                                                     ctGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCCAGCAGGCCGGtg   <  1:1084746/101‑1 (MQ=255)
                                                                                      tGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCCAGCAGGCCGGtgc  >  3:1484134/1‑101 (MQ=255)
                                                                                             |                                                                                             
GCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCCAGCAGGCCGGTGC  >  NC_007779/649136‑649322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: