Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779	649,229	A→T	L162H (CTC→CAC)	citE ←	citrate lyase, citryl‑ACP lyase (beta) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	649,229	0	A	T	100.0%	105.6 / NA	27	L162H (CTC→CAC)	citE	citrate lyase, citryl‑ACP lyase (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (10/17);  total (10/17)

GCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCCAGCAGGCCGGTGC  >  NC_007779/649136‑649322                                                                                              |                                                                                              gcgGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGAt                                                                                        <  3:1178013/101‑1 (MQ=255)      cTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGa                                                                                   >  3:1368466/1‑101 (MQ=255)            aaTGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAAc                                                                             <  4:28262/101‑1 (MQ=255)                    aGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGaa                                                                     <  3:281965/101‑1 (MQ=255)                            aaCAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCCCCGTGGGCGATACCGCTCAAACGCTCGGAAGCGTGAGc                                                             <  1:1578941/101‑1 (MQ=255)                             aCAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCg                                                            <  3:949092/101‑1 (MQ=255)                               agcagTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGAt                                                          >  3:455104/1‑101 (MQ=255)                                   gttcagttcCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTcc                                                      <  3:411843/101‑1 (MQ=255)                                    ttcagttcCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCa                                                     >  3:861639/1‑101 (MQ=255)                                            cttccGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACAGATCAATCGCTCGGTAGCGTGAGCGATTTCCACTGCGCgg                                             <  3:1552102/101‑1 (MQ=255)                                                   ggAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATcc                                      <  3:1107414/101‑1 (MQ=255)                                                       cGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAg                                  >  1:251462/1‑101 (MQ=255)                                                          tCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCgg                               >  3:359719/1‑101 (MQ=255)                                                           cTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGga                              <  1:884454/101‑1 (MQ=255)                                                             gTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGgaga                            <  3:971930/101‑1 (MQ=255)                                                             gTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGgaga                            >  3:214699/1‑101 (MQ=255)                                                             gTACGCAGGTTGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGgaga                            <  3:719398/101‑1 (MQ=255)                                                                      ttGCGCACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATcgc                   <  1:541337/101‑1 (MQ=255)                                                                        gcgcACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATcgccg                 >  3:787382/1‑101 (MQ=255)                                                                         cgcACATAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATcgccgc                <  1:81135/101‑1 (MQ=255)                                                                           cacaTAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCca              <  1:821190/101‑1 (MQ=255)                                                                             caTAGTCTTCCGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCCCcacc            >  1:1584802/1‑99 (MQ=255)                                                                              aTAGTCTTCTGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCcagca           <  1:357094/101‑1 (MQ=255)                                                                                    ttctGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCCAGCAGGCCgg     <  1:1354888/101‑1 (MQ=255)                                                                                     tctGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCCAGCAGGCCGGt    >  3:937195/1‑101 (MQ=255)                                                                                      ctGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCCAGCAGGCCGGtg   <  1:1084746/101‑1 (MQ=255)                                                                                       tGCACCGTGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCCAGCAGGCCGGtgc  >  3:1484134/1‑101 (MQ=255)                                                                                              |                                                                                              GCGGCCTGCAAAATGGAACAGCGTGCGAACAGCAGTTCAGTTCCTTCCGGGGAGCGTTCTGTACGCAGGTTGCGCACATAGTCTTCTGCACCGAGGGCGATACCGATCAAACGCTCGGAAGCGTGAGCGATTTCCACTGCGCGGGTAATCCCCAGCGGAGATTCAATCGCCGCCAGCAGGCCGGTGC  >  NC_007779/649136‑649322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑