Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779	63,992	G→A	H597Y (CAT→TAT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	63,992	0	G	A	100.0%	242.6 / NA	58	H597Y (CAT→TAT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/26);  total (32/26)

CCGCTGCTTGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATGGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGA  >  NC_007779/63897‑64087                                                                                                |                                                                                                ccGCTGCTTGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGttt                                                                                            >  3:590331/1‑101 (MQ=255)  cGCTGCTTGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTc                                                                                           >  1:1324647/1‑101 (MQ=255)  cGCTGCTTGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTc                                                                                           <  1:1106607/101‑1 (MQ=255)  cGCTGCTTGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTc                                                                                           >  1:18875/1‑101 (MQ=255)   gctgctTGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCa                                                                                          <  3:1261798/101‑1 (MQ=255)      gctTGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATAc                                                                                       <  1:622728/101‑1 (MQ=255)         tGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTcc                                                                                    >  1:1021/1‑101 (MQ=255)            cGCCCTCCTGCATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGt                                                                                 >  4:82121/1‑101 (MQ=255)               cctcctGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCt                                                                              >  3:589733/1‑101 (MQ=255)                 tcctGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTaa                                                                            >  3:1021437/1‑101 (MQ=255)                    tGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATgc                                                                         <  1:722575/101‑1 (MQ=255)                    tGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATgc                                                                         <  1:571757/101‑1 (MQ=255)                     gAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATgcg                                                                        <  1:841252/101‑1 (MQ=255)                         cAGTCCGGCATAACACTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTgg                                                                    <  1:1537865/101‑1 (MQ=255)                           gTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTc                                                                  >  3:358181/1‑101 (MQ=255)                                 cATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCgt                                                            >  1:779773/1‑101 (MQ=255)                                  aTAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCgtt                                                           <  3:5037/101‑1 (MQ=255)                                      cGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGttt                                                       <  1:925314/101‑1 (MQ=255)                                        cTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAGCGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGttttt                                                     <  1:805271/101‑1 (MQ=255)                                               cTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGt                                              >  3:166665/1‑101 (MQ=255)                                               cTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGt                                              <  1:490433/101‑1 (MQ=255)                                                tGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGtt                                             >  3:1148854/1‑101 (MQ=255)                                                tGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGtt                                             >  3:802158/1‑101 (MQ=255)                                                 gCCGGTATCGGCTCCGCGCATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGttt                                            >  4:100659/1‑101 (MQ=255)                                                 gCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGttt                                            >  3:1460878/1‑101 (MQ=255)                                                  ccGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTc                                           >  1:888905/1‑101 (MQ=255)                                                  ccGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTc                                           <  3:495880/101‑1 (MQ=255)                                                   cGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTcc                                          <  1:1339419/101‑1 (MQ=255)                                                    ggTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCg                                         >  3:821712/1‑101 (MQ=255)                                                      tATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGcc                                       >  1:1118394/1‑101 (MQ=255)                                                       aTCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGccc                                      <  3:749964/101‑1 (MQ=255)                                                       aTCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGccc                                      <  1:49766/101‑1 (MQ=255)                                                       aTCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGccc                                      >  1:1557661/1‑101 (MQ=255)                                                       aTCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGccc                                      <  1:334143/101‑1 (MQ=255)                                                        tCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCcca                                     <  1:881123/101‑1 (MQ=255)                                                        tCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCcca                                     <  1:639655/101‑1 (MQ=255)                                                         cGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCccac                                    >  3:892063/1‑101 (MQ=255)                                                          ggCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCccacc                                   <  2:100765/101‑1 (MQ=255)                                                           gCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCccacca                                  >  1:1285075/1‑101 (MQ=255)                                                             tCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAgg                                >  1:736125/1‑101 (MQ=255)                                                              ccGCGAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGc                               <  1:375185/101‑1 (MQ=255)                                                                 cgAATGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGtt                            <  1:1011701/101‑1 (MQ=255)                                                                   aaTGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTaa                          >  1:507903/1‑101 (MQ=255)                                                                   aaTGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTaa                          >  1:667456/1‑101 (MQ=255)                                                                   aaTGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTaa                          <  1:67150/101‑1 (MQ=255)                                                                    aTGGTTGGCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAAc                         >  1:1511256/1‑101 (MQ=255)                                                                          ggCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGtgctg                   >  3:842146/1‑101 (MQ=255)                                                                           gCATCAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGtgctgc                  <  3:787845/101‑1 (MQ=255)                                                                             aTCAGAAAACGGCAGAAATAGGTTTCATTCTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCAc                <  1:742338/101‑1 (MQ=255)                                                                               cAGAAAACGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCa              >  1:653967/1‑101 (MQ=255)                                                                                  aaaaCGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTg           >  3:101874/1‑101 (MQ=255)                                                                                  aaaaCGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTg           >  3:1365274/1‑101 (MQ=255)                                                                                    aaCGGCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCa         <  1:1580932/101‑1 (MQ=255)                                                                                       ggCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGa      >  3:293533/1‑101 (MQ=255)                                                                                        gCAGAAATAGGTTTCATGCTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGAc     <  3:55760/101‑1 (MQ=255)                                                                                        gCAGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGAc     >  1:1085387/1‑101 (MQ=255)                                                                                          aGAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCg   >  1:33402/1‑101 (MQ=255)                                                                                           gAAATAGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGa  >  1:785799/1‑101 (MQ=255)                                                                                                |                                                                                                CCGCTGCTTGTCGCCCTCCTGAATCAGTCCGGCATAACGCTTTTTACTGCCGGTATCGGCTCCGCGAATGGTTGGCATCAGAAAACGGCAGAAATGGGTTTCATACTCCAGTTCTAATGCGCTGGTCAGCCGTTGTTTTTGCAGCGTTTCCGCCCACCAGGCGTTAACGTGCTGCACCAGTGCACGACCGA  >  NC_007779/63897‑64087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑