Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077791,164,9440TG42.4% 43.2 / 11.7 34S36R (AGT→AGGycfNthiamin kinase
Reads supporting (aligned to +/- strand):  ref base T (2/17);  new base G (14/0);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.47e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCAC  >  NC_007779/1164850‑1165044
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gcaATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTtttc                                                                                                >  1:1132066/1‑101 (MQ=255)
         ccATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACAt                                                                                       <  3:564492/101‑1 (MQ=255)
         ccATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACAt                                                                                       <  3:104629/101‑1 (MQ=255)
                    gacgaATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTTCTCATTTAACATCAGGGCCAGCg                                                                            >  3:122237/1‑101 (MQ=255)
                     acgaATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGt                                                                           <  3:1531858/101‑1 (MQ=255)
                       gaATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTTCTCATTGAACATCAGGGCCCGCGgtt                                                                         >  1:415932/1‑101 (MQ=255)
                              tGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTtgtg                                                                  <  4:58084/101‑1 (MQ=255)
                                tCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCg                                                                <  1:435047/101‑1 (MQ=255)
                                   cgcTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGGGTTTTTATTTAAGATCAAGAGCACCGCTttgtgttggtgtt                                                             >  3:1398922/1‑92 (MQ=255)
                                    gcTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTTCTCCTTTAACATCAGGGCCAGCGGTTTGTTGTGCGTCAg                                                            >  1:352618/1‑101 (MQ=255)
                                      tttttCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGcc                                                          <  3:237353/101‑1 (MQ=255)
                                      tttttCCCGCAGTATCATCCCGTCACGACGTCTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGcc                                                          <  3:1588786/101‑1 (MQ=255)
                                          tCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTGTTCTCATTTGACATCATGACCAACGTATTGTTGTTGGTTAGCCGCAc                                                      >  2:74089/1‑101 (MQ=255)
                                          tCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGGTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCAc                                                      >  2:128660/1‑101 (MQ=255)
                                              gCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGATTTCTCCTTTAAACAAAGAGCCAGCGCTTTGTTTTGCGTCTGCCGCAcagcc                                                  >  1:1389478/1‑97 (MQ=255)
                                                aGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTTTTCATTTAAAATCAAGGCCCGCGGTTTTTTTTGGGTCAGCCGCCCGCTccc                                                >  3:34076/1‑100 (MQ=255)
                                                      atcCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTTCTCATTTTACATCAGGGCCCGCGTTTTGTTGTGCGTCAGGCGCCCCATCCTCGTgcg                                          >  3:662142/1‑101 (MQ=255)
                                                         ccGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTTTTCTTTTAACAACAGGGCCAGCGTTTTGTTTTGCGTCAGCCGCACGATCCTCATGcgccc                                       >  3:1352042/1‑100 (MQ=255)
                                                         ccGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTTCTCCTTTAACATCAGGGCCAGCGGTTTTTTGTGCGTCAGCCGCACGATCCTGATGcgccc                                       >  3:328351/1‑100 (MQ=255)
                                                          cGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGGTTTTTCATTGAACATCAGGGCCAGCGGTTTGTTGTGGGTCAGCCGCACCAGCCCGATGcgccgc                                      >  1:37205/1‑101 (MQ=255)
                                                            tCACGACGTTTAATAGTGGGCTTAGTGGCGGGGGTTTTCTCATTTAAAAACAAGGCCAAAGTTTTGTTTTGCGTCAGCCGCCCGAACCTCCTGCGCCGCAg                                    >  1:1407680/1‑101 (MQ=255)
                                                                gacgTTTAATAGTGGGCTTAGTGGCGGGGGGTTTTTCATTGAAAATCAGGGGCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGGCcg                                >  3:671215/1‑101 (MQ=255)
                                                                  cgTTTAATAGTGGGCTTAGTGGCGGGAGGTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCcgcg                              >  1:256893/1‑101 (MQ=255)
                                                                     ttAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTc                           <  1:179251/101‑1 (MQ=255)
                                                                      tAATAGTGGGCTTAGTGGCGGGGGGTTTTTTATTTAAAAATAGGGCCCGCGCTTTTTTTTTCGGCAGCCGCCCCACCCCGATGCGCCGCAGGcccccgtct                          >  3:810149/1‑94 (MQ=255)
                                                                      tAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCt                          <  3:451250/101‑1 (MQ=255)
                                                                       aaTAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCtt                         <  1:121023/101‑1 (MQ=255)
                                                                             gggCTTAGTGGCGGGATTTTTCTCATTGGACATCAGGGGCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCCAATGCGCCGCACTCCGCGTTCTTGCGCCa                   <  1:331102/101‑1 (MQ=255)
                                                                                  tAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCCGTCCGCGTTCTTGCGCCAGTATc              <  3:1596544/101‑1 (MQ=255)
                                                                                       gCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCt         <  1:1396723/101‑1 (MQ=255)
                                                                                       gCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCt         <  1:1142114/101‑1 (MQ=255)
                                                                                       gCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCt         <  1:490758/101‑1 (MQ=255)
                                                                                        cGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCtt        <  1:805343/101‑1 (MQ=255)
                                                                                          ggAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTa      <  1:597243/101‑1 (MQ=255)
                                                                                              ttttCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATcac  <  3:1077265/101‑1 (MQ=255)
                                                                                              ttttCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATcac  >  3:938952/1‑101 (MQ=255)
                                                                                              ttttCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATcac  >  3:1041185/1‑101 (MQ=255)
                                                                                              |                                                                                                    
GCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCAC  >  NC_007779/1164850‑1165044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: