| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007779 | 357,146 | 0 | C | G | 43.3% | 38.4 / 13.2 | 30 | A257P (GCC→CCC) | cynR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (3/14); new base G (13/0); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.79e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCT > NC_007779/357046‑357242 | cATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc > 1:706154/1‑101 (MQ=255) ttATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGcggg > 1:1160370/1‑98 (MQ=255) aaaaaTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑GAAAAAGCTTTTAGccc > 1:482284/1‑101 (MQ=39) aaaaaTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGccc < 3:343615/101‑1 (MQ=255) cTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGGGA‑GAAAAAACTTTAAAccccccctgtt > 3:623483/1‑93 (MQ=255) cTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGGAAAAAGATTT‑AGCCCGTCCtgtt > 1:285294/1‑101 (MQ=255) tCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAAA‑AGAAAACCTTTTATACCGTCTtgttgt > 1:1052314/1‑101 (MQ=255) ggcTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCaa < 3:392359/101‑1 (MQ=255) gcTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑GAAAAAACATTAAAACCCGCATGTTTTGTGGGaaa > 3:1138747/1‑100 (MQ=255) tGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑GAAAAAACCTTAAACCCGTCCTGTTGTGTGGGGATCg > 1:314018/1‑101 (MQ=255) gTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑GAAATAGCTTTTAACCCCTCCTCTTGTGTGGCGATCgc > 3:1591495/1‑101 (MQ=255) tCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑GAAAAAGCTTTAAACCCGTCATGTTGTGTGGGAAACgct > 4:36124/1‑101 (MQ=255) cTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑GAAAAAGATTTAAGCCCGGCATGTTGTGTGGCGATCgctg > 1:1464916/1‑101 (MQ=255) gCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctgct < 1:839651/101‑1 (MQ=255) ccAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGctgctg < 1:1484855/101‑1 (MQ=255) tATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAc < 3:1542138/101‑1 (MQ=255) aTTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACa < 3:1215976/101‑1 (MQ=255) ccgcAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGaaa < 1:84305/101‑1 (MQ=255) caacaaAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑GAAAAAGCTTTAAAACCCTCATGTTGTGTGGGGATTGCTGCTGGTGACAATGTGGGAAggg > 1:1211270/1‑101 (MQ=255) aacaaAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGGGA‑GAAAAAAAGTTAAGACCCCCAAGTTGTGGGGCCAGCTCCGCCTGTAACAATGTGGAAAgggg > 3:1490676/1‑100 (MQ=255) caaAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑GAAATAGCATTTAGCCCCCCACTTTTTGTGGGACATGCTGCTGGTGACAATGTGGGAAGGGGAg > 1:1547328/1‑101 (MQ=255) aaaaCCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑GAAAAAGCTTTTAACCCCGCCTGTTGTGTGGCAAACGCTGCTTGTGACAAAGGGGGAAGGGGAga > 1:1259779/1‑100 (MQ=255) aaaCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTg < 3:713237/101‑1 (MQ=255) tctctcCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCa < 1:5883/101‑1 (MQ=255) cAGTAGTGGCGGGGCGAGA‑AGAAGAACTTTAAAGCCGTCTCGTTTTGTGGCAATCCCTGCTGGTAACAATGTGGGAAGGGGAGGGCCGGGGATCAGcctcc > 1:781222/1‑97 (MQ=255) cAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCa < 1:1131615/101‑1 (MQ=255) agtagtGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAg < 1:216690/101‑1 (MQ=255) tagtGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGaa < 1:839635/101‑1 (MQ=255) agtGGCGGGGCAAGA‑GAAAAAAATTTAAACCCCCCATGTTTTGTGGGAATCGCTGCTGGTAACAATGTGGGAAGGGGAGGGGGGGGGAACAGCTCCCGaaa > 1:114679/1‑100 (MQ=255) tGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcg < 3:1280643/101‑1 (MQ=255) cGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCgcgc > 3:181591/1‑101 (MQ=255) ggggCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCt < 3:38923/101‑1 (MQ=255) | CATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCT > NC_007779/357046‑357242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |