Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_007779 | 298,184 | 0 | A | C | 42.3% | 72.7 / 19.6 | 53 | Y659D (TAT→GAT) | yagR | oxidoreductase with molybdenum‑binding domain |
Reads supporting (aligned to +/- strand): ref base A (22/8); new base C (0/22); total (22/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.20e-08 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
CGACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGAC > NC_007779/298088‑298283 | cGACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATAcccc < 3:846930/101‑1 (MQ=255) gACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCg < 3:333964/101‑1 (MQ=255) cacCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGcc < 1:348695/101‑1 (MQ=255) ccTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCat < 3:543224/101‑1 (MQ=255) tGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCg > 1:1457919/1‑101 (MQ=255) ggCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATCCCCCGCCATATCGt < 3:622858/101‑1 (MQ=255) ttCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCCCCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGAtt < 1:303866/101‑1 (MQ=255) ttCATCGGGGAGGATATGGGGCCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACACCGCCATATCGTGAtt > 1:204033/1‑101 (MQ=255) cATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTaa > 1:968720/1‑101 (MQ=255) cATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTaa > 1:480011/1‑101 (MQ=255) aTCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAAc > 1:26507/1‑101 (MQ=255) tCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCCCCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATCCCCCGCCATATCGTGATTAACg < 3:854319/101‑1 (MQ=255) tCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACg > 1:244031/1‑101 (MQ=255) ggggAGGATATGGGGTCGGTATCATCCAGGAAAATCCCCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGaa < 1:616405/101‑1 (MQ=255) ggggAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGaa > 1:741625/1‑101 (MQ=255) ggaggaTATGGGGTCGGTATCATCCAGGAAAATCCCCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGt < 1:556809/101‑1 (MQ=255) aggaTATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATCCCCCGCCATATCGTGATTAACGAAGTAg < 3:1357605/101‑1 (MQ=255) atatGGGGTCGGTATCATCCAGGAAAATCCCCTCCTGTTTTGGGATATCCGCATGAACCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGccc < 1:1061053/101‑1 (MQ=255) tatGGGGTCGGTATCATCCAGGAAAATCCCCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCa < 3:1537423/101‑1 (MQ=255) atGGGGTCGGTATCATCCAGGAAAATCCCCTCCTGTTTTGGGATATCCGCATGAACCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCaa < 1:1350614/101‑1 (MQ=255) tagagccGGTATCATACAGGAAAATCGCCTCCTATTTTGGAATACCCGGATGAACCGNCACCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCaaa < 3:1154079/95‑1 (MQ=255) tGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGACCCGGCCCCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCaaa < 1:1540948/101‑1 (MQ=255) ggggTCGGTATCATCCAGGAAAATCCCCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAAc < 3:635608/101‑1 (MQ=255) ggggTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAAc > 3:382357/1‑101 (MQ=255) tATCATCCAGTAAACCCTCCCCCTGTTTTGGCATATCCCCAAGAGCGGGCACCACTTTCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATcc < 1:1190880/101‑1 (MQ=255) acatcCAGGCAACTCTCCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCAc > 4:59841/2‑101 (MQ=255) atcCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCAGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCg > 1:884796/1‑101 (MQ=255) tcCAGGAAAATCCCCTCCTGTTTTGGGATATCCGCATGACCCGGCACCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGc < 3:1346229/101‑1 (MQ=255) aGGAAAATCCCCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAg < 3:386086/101‑1 (MQ=255) aGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAg > 3:1564665/1‑101 (MQ=255) aaaaTCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGctc > 1:1127972/1‑101 (MQ=255) aaaTCCCCTCTTGTTTTGGGATATCCGCATGACCCGGCACCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGctcc < 3:1087928/101‑1 (MQ=255) tcccctcctGTTTTGGGATATCCGCATGAACCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCtcctcc < 3:1474408/98‑1 (MQ=255) aCCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCAt > 1:828852/1‑101 (MQ=255) ctcctGTTTTGGGATACCCGCATGAACCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCa < 3:611067/101‑1 (MQ=255) ctcctGTTTGGGGATATCCGCATGACCCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCGCCGCCATCa < 4:85326/101‑1 (MQ=255) tcctGTTTTGGGATATCCGCATGAACCGGCACCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAg < 3:954903/101‑1 (MQ=255) cttGTTTTGGGATATCCGCATGAACCGCCACCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGt < 3:35184/99‑1 (MQ=255) ctGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTg > 3:1111928/1‑101 (MQ=255) ttttGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCcg > 1:735/1‑101 (MQ=255) ttGGGATAGCCGAATGAACCGGCACCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCcgcg < 3:173997/101‑1 (MQ=255) tatCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCgcccatg > 1:846928/1‑101 (MQ=255) tCCGCATGACCCGGCACCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCgcccatgcc < 3:122822/101‑1 (MQ=255) ccGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCgcccatgccc > 1:1539251/1‑101 (MQ=255) ctaatgccccGCCACCCCATCCCCCGCCATATCGTGATTAACGAATTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGAGCCGCtcccatgccca < 1:735671/93‑1 (MQ=255) gCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCAGCgcccatgcccat > 1:599257/1‑101 (MQ=255) tGAACCGGCACCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGt < 3:1403558/101‑1 (MQ=255) ggCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTgc < 1:352321/101‑1 (MQ=255) cACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTgcgc > 3:1163685/1‑101 (MQ=255) aCCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGcc > 3:597292/1‑101 (MQ=255) tCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAAt < 1:9608/101‑1 (MQ=255) cATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATg > 1:900257/1‑101 (MQ=255) tACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGAc > 1:1537124/1‑101 (MQ=255) | CGACACCTTTGGCCTTCATCGGGGAGGATATGGGGTCGGTATCATCCAGGAAAATCACCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGAC > NC_007779/298088‑298283 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |