Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077794,639,6580GA41.9% 66.8 / 16.5 43A111A (GCC→GCT) robDNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (6/19);  new base A (18/0);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 2.28e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGC  >  NC_007779/4639560‑4639757
                                                                                                  |                                                                                                   
ctctGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGgc                                                                                                   <  3:995843/101‑1 (MQ=255)
      gTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCa                                                                                             <  3:1008617/101‑1 (MQ=255)
      gTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCa                                                                                             <  3:787706/101‑1 (MQ=255)
       tAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCAt                                                                                            <  3:1215205/101‑1 (MQ=255)
         acacCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTc                                                                                          <  1:1443700/101‑1 (MQ=255)
          cacCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGGGGATAACAAAAGAGCTCCCTTcc                                                                                         >  1:191747/1‑100 (MQ=255)
            ccAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGGATACCAAAAGCGCTcccctcacg                                                                                       >  2:10189/1‑94 (MQ=255)
            ccAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAgg                                                                                       <  3:729683/101‑1 (MQ=255)
            ccAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAgg                                                                                       <  1:378411/101‑1 (MQ=255)
              aaTCAGCGGCGTCTCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGGGGAAACCAAAAGCGCCCCCTTCAGgag                                                                                     >  4:118927/1‑101 (MQ=255)
                  aGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGGGGATAACAAAAGCGCCCCCCTCCAGAGAAcg                                                                                 >  1:417030/1‑101 (MQ=255)
                     ggcgTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAAcggcg                                                                              <  3:193127/101‑1 (MQ=255)
                      gcgTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGGGGATAACAAAAGCGGCCCACTCAGGAGAAaggcgg                                                                             >  3:1066240/1‑101 (MQ=255)
                          aTCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGGGGATACCCCAAGCGCCCCCCTCAGGAGAAcgggggggaa                                                                         >  3:1134717/1‑97 (MQ=255)
                            cTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGGAAACCAAAAGCGGCCCACTCAGGAGAAAGGCGGGAAAgg                                                                       >  1:1463147/1‑100 (MQ=255)
                            cTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAAATCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGt                                                                       <  1:805612/101‑1 (MQ=255)
                             ttCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGGGAAAAACCAAAGAGCGCCATTCACGAAGAcagcggcgaaaag                                                                      >  3:1477018/1‑94 (MQ=255)
                               ccAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCa                                                                    <  3:345896/101‑1 (MQ=255)
                                 aGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGGATACCAAAAGCGCCCCATTCAGGAGAACGGCGGGGAAGGGCAgg                                                                  >  3:149818/1‑101 (MQ=255)
                                          aaTTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGc                                                         <  1:611997/101‑1 (MQ=255)
                                            tttGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCATGAGGCGGGGCaa                                                       <  4:10184/101‑1 (MQ=255)
                                             ttGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGGAAAACAAAAGCGCCCCCTTCAGGAGAACGGCGGGGAAGGGGAGGGGGCTggggcaa                                                      >  1:548215/1‑97 (MQ=255)
                                               gtgCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGGAAACCAAAAGCGCCCCCTTCAGGAGAAAGGGGGGGAAAAGGAGGAGGCTGGGGaaaat                                                    >  3:22823/1‑99 (MQ=255)
                                                  ctctGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGGGGATACCAAAAGCGCCCCCTTCAGGAGGACGGCGGGGAAGAGCAGGGGGGTGGGGAAAATGct                                                 >  1:443088/1‑101 (MQ=255)
                                                  ctctGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGGAAACCAAAAGGGCCCCCTTCAGGAGAACGGCGGGGAAGGGGAGGGGGCTGGGGaaaaaggt                                                 >  1:1350141/1‑96 (MQ=255)
                                                   tctGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGGGGATACCCAAAGCGCCCCCTTCAGGAGGACGGCGGGAAAGGGCAGGAGGCTGGGCCAACTGcct                                                >  3:712468/1‑99 (MQ=255)
                                                    ctGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGcttc                                               <  2:142928/101‑1 (MQ=255)
                                                      ggCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTATGGGCAAACTGcttctt                                             <  1:1319994/101‑1 (MQ=255)
                                                              gAATTCACCCAGGCGTAGCGGCGGGCGAAAAACAAAAGGGCCCCCTTCAGGGGAAAGGCGGGAAAAGGGAGGGGGCTGGGCAAAACGCTTCTTGAATacgc                                     >  1:225299/1‑101 (MQ=255)
                                                                  tCACCCAGGCGTAGCGGCGGGCGAATACCAAAAGCGCCCCATTCAGGGGAACGGCGGGGAAAAGGAGGGGGCTGGGGGAAACGCTTCTTTGATGCGCgggg                                 >  1:1338236/1‑100 (MQ=255)
                                                                      ccAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAAt                             <  3:698962/101‑1 (MQ=255)
                                                                      ccAGGCGTAGCGGCGGGCGAATACCAAAAGCGCTCCATTCAGGAGAACGGCGGGAAAAGGCAGGAGGCTGGGCAAACTGCTTCTTGAATGCGCGGGGAAAt                             >  1:165517/1‑101 (MQ=255)
                                                                       cAGGCGTAGCGGCGGGCGAAAACCAAAAGCGCCCCATTCAGGAGAAAGGCGGGAAAGGGCAGGAGGCTGGGCAAACTGCTTCTTTAATGCGCGGGGGAATg                            >  1:1106797/1‑101 (MQ=255)
                                                                              aGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTg                     <  1:408144/101‑1 (MQ=255)
                                                                              aGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTg                     <  1:118806/101‑1 (MQ=255)
                                                                                    gggcgAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTc               >  1:1541367/1‑101 (MQ=255)
                                                                                    gggcgAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTc               >  1:85055/1‑101 (MQ=255)
                                                                                        gAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAg           <  1:1569722/101‑1 (MQ=255)
                                                                                        gAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAg           >  1:597630/1‑101 (MQ=255)
                                                                                          aTACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCg         >  3:1528772/1‑101 (MQ=255)
                                                                                           tACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCgg        >  3:1088749/1‑101 (MQ=255)
                                                                                             ccAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTGGAGAGTCGAATCGGTa      <  4:120265/101‑1 (MQ=255)
                                                                                                 aGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGc  >  3:1307511/1‑101 (MQ=255)
                                                                                                  |                                                                                                   
CTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGC  >  NC_007779/4639560‑4639757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑