Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077792,648,5950AC47.2% 39.3 / 19.8 37G783G (GGT→GGGyfhMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (16/3);  new base C (0/17);  total (16/21)
Fisher's exact test for biased strand distribution p-value = 1.35e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCG  >  NC_007779/2648497‑2648691
                                                                                                  |                                                                                                
ttATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTAcc                                                                                                <  1:751340/101‑1 (MQ=255)
  aTCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACccg                                                                                              <  3:918141/101‑1 (MQ=255)
      cccGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCCCCACAGCGGCCCTTCACTGGACTCGACCATCGCATACCCTTTACCCGCCGt                                                                                          <  1:1278096/100‑1 (MQ=255)
         ggAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCCCCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTCCCCGCCGTTgg                                                                                       <  1:330859/101‑1 (MQ=255)
          gAATCGTCAGATCCAGCCCTTGAGCCCGAACTTCAATCTCTTGCCCCCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTCCCCGCCGTTGgc                                                                                      <  1:163144/101‑1 (MQ=255)
                tCAGACCCAGCCCTTGAGCCCAAACATCAATCTCTTGCCCCCACAGCGGCCCTTCACGGGACTCGACCATCGCATACCCTTTCCCCGCCGTTGGCgcggcg                                                                                <  1:948087/101‑1 (MQ=255)
                  agcccccccccTGGACCCCAAACATCATTCTCTTCCCCCCCCGGCGCCCCTTCCCTGGCCTCGCCCACCGCATACCCTTTCCCCGCCGTTGGCGCGGCGat                                                                              <  1:14060/93‑1 (MQ=255)
                           ccTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACtt                                                                     >  3:90916/1‑101 (MQ=255)
                           ccTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACtt                                                                     >  1:1530778/1‑101 (MQ=255)
                                 gCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGgt                                                               >  1:430171/1‑101 (MQ=255)
                                   ccGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGgtgt                                                             >  3:1411767/1‑101 (MQ=255)
                                    cGAACATCAATCTCTTGCCCCCACGGCGGCCCTTCCCTGGACTCGCCCATCGCATACCCTTTCCCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTc                                                            <  1:1548689/101‑1 (MQ=255)
                                     gAACATCAATCTCTTGCCCCCACGGCGGCCCTTCACTGGCCTCGACCATCGCATACCCTTTCCCCGCCGTTGGCGCGGCGATATGCAACGGAAGGgtgtgg                                                           <  4:12410/101‑3 (MQ=255)
                                       aCATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGcc                                                         >  3:677056/1‑101 (MQ=255)
                                          tCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAgg                                                      >  1:1285346/1‑101 (MQ=255)
                                               ctctTCCCCCCCCGGCGCCCCTTCACGGGCCTCGCCCATCGCATACCCTTTCCCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGAt                                                 <  1:962216/101‑1 (MQ=255)
                                                 ctTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATaa                                               >  1:852649/1‑101 (MQ=255)
                                                    cccaccccaccGTCCCTACACGGGACTCCCCCACCGCTTACCCTTGTCCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTg                                            <  3:1271430/100‑1 (MQ=255)
                                                     cccccccggcgcCCCTTCACGGGACTCGACCATCGCATACCCTTTCCCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTgg                                           <  1:319341/93‑1 (MQ=255)
                                                     ccaccaCAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTgg                                           >  3:1582189/1‑101 (MQ=255)
                                                                    ttCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCa                            >  1:397676/1‑101 (MQ=255)
                                                                    ttCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCa                            >  1:182085/1‑101 (MQ=255)
                                                                    tccccTGGCCCCGCCCACCGAATACCCTTCCCCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCa                            <  1:947283/97‑1 (MQ=255)
                                                                      ccctgcccTCGCCCACCGCATACCCTTTCCCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGc                          <  1:711111/94‑1 (MQ=255)
                                                                      cACTGGACTCGACCACCGCATACCCTTTCCCCGCCGTTGGCGCGGTGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGc                          <  3:1245674/101‑1 (MQ=255)
                                                                       tcggcaCTCGCCCTTCCCATATCCTTTCCCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCg                         <  1:117476/96‑1 (MQ=255)
                                                                       aCTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCg                         >  1:728442/1‑101 (MQ=255)
                                                                       aCTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCg                         >  1:1257610/1‑101 (MQ=255)
                                                                       aCTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCg                         >  1:1391754/1‑101 (MQ=255)
                                                                         gggccTCGCCCACCCCATACCCTTCCCCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTg                       <  1:293828/97‑1 (MQ=255)
                                                                           gccgcgcccACCGCATACCCTTTCCCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGac                     <  3:1200357/94‑1 (MQ=255)
                                                                                gcccATCGCATACCCTTCCCCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACggt                <  1:718879/99‑1 (MQ=255)
                                                                                      cGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACggtcgggtc          >  1:1262565/1‑101 (MQ=255)
                                                                                         aTAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCa       >  1:803024/1‑101 (MQ=255)
                                                                                              ttttCCCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTgcg  <  1:1109526/100‑1 (MQ=255)
                                                                                              cTTTCCCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTgcg  <  1:694183/101‑1 (MQ=255)
                                                                                              cTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTgcg  >  3:434143/1‑101 (MQ=255)
                                                                                                  |                                                                                                
TTATCGACCGGAATCGTCAGATCCAGCCCTTGAGCCCGAACATCAATCTCTTGCCACCACAGCGGCCCTTCACTGGACTCGACCATCGCATAACCTTTACCCGCCGTTGGCGCGGCGATATGCAACTTAATGGTGTCGCCAGGGCGATAACTGGCTTTATCCAGTTTCAGCGTGACACGGTCGGGTCGCACTGCG  >  NC_007779/2648497‑2648691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: