| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007779 | 2,225,700 | 0 | T | G | 42.2% | 64.1 / 16.7 | 45 | V61G (GTG→GGG) | dld | D‑lactate dehydrogenase, FAD‑binding, NADH independent |
| Reads supporting (aligned to +/- strand): ref base T (2/24); new base G (18/1); total (20/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.96e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATC > NC_007779/2225607‑2225798 | cAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGCTGgaa > 3:804380/1‑101 (MQ=255) aaaaaCGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAg < 3:667235/101‑1 (MQ=255) aaaaCGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGc < 1:1283238/101‑1 (MQ=255) gCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCAcc < 3:1265109/101‑1 (MQ=255) tATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTTGTGTAAACACGCGCCAccgc > 3:294865/1‑101 (MQ=255) tATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCAccgc < 1:774172/101‑1 (MQ=255) aTCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGCCAccgcc < 1:1255735/101‑1 (MQ=255) gggCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACaaaa < 1:1097115/101‑1 (MQ=255) ttCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGCTGAAAAACCGCGGCACCGCCCCCAAAaatat > 1:163039/1‑101 (MQ=255) ttCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGCGGGAAGCCCGCGTCACCGCCGACAAAaatat > 1:1044516/1‑101 (MQ=255) ccGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGGTGAAAGCCTGCGTCACCCCCGACAAAaatattt > 3:953004/1‑100 (MQ=255) ccGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGCTGGAAGCCTGCGTCACCCCCCGCAAAaatattt > 3:24770/1‑100 (MQ=255) ccGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGCTGGAAAACCGCGGCCCCCCCCACAAAaatattt > 3:332623/1‑100 (MQ=255) ccGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGCTGAAAGCCCGCGGCGCCCCCGACAAAaatattt > 1:403592/1‑100 (MQ=255) cGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGCTGAAAGACCGCGTCACCGCCCCCAAAAATATTCt > 1:675998/1‑101 (MQ=255) gTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGGGGGGGAAAGCC‑GCGTCACCCCCGACAAAAATATTCtt > 3:946730/1‑100 (MQ=255) ttCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGa < 3:337875/101‑1 (MQ=255) tCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGCTGAAAGCCCGCGGCACCCCCGGCAAAattaattttat > 1:732258/1‑94 (MQ=255) cTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGCTGAAAGACCGCGGCACCGCCGCCAAAAATATTCTGATg > 4:30394/1‑101 (MQ=255) tCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAgg < 1:712614/101‑1 (MQ=255) tCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAgg < 1:208036/101‑1 (MQ=255) tCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGGTGAAAACCCGCGTCACCCCCCCCAAAAATATTCTTATGCAgg > 3:333775/1‑101 (MQ=255) cGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCa < 3:93749/101‑1 (MQ=255) gcgcTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGCCGCAAGACTCGGTCACCCCCAACCAAATTTTTCTTCAGAAGGCCCCCACTAc > 3:216474/1‑101 (MQ=255) ggctgTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGGTGAAAACCTGCGGCACCGCCGACAAAAATATTCTTATGCAGGCCGCCacaaaaagcc > 1:575416/1‑92 (MQ=255) cGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCg < 3:108170/101‑1 (MQ=255) ttttCCCTGGCTCACTACTAGAATTGTGGC‑GGGGGCTGAAAAACCGCGTCACCGCCCACAAAAATATTCTGATGCAGGCCGCCAATACAGGCCCGACCGaa > 1:556481/1‑101 (MQ=255) tCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGa < 1:653226/101‑1 (MQ=255) tCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGa < 1:1503321/101‑1 (MQ=255) tCCCTCGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGa < 1:1038300/101‑1 (MQ=255) ggCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGAc < 2:6633/101‑1 (MQ=255) gCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCGGGCCGCCAATACAGGCCTGACCGAAGGATCGCCg < 1:373150/101‑1 (MQ=255) gCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACg < 1:330364/101‑1 (MQ=255) gCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACg < 1:24472/101‑1 (MQ=255) cTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGc < 3:1507373/101‑1 (MQ=255) cTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGc < 3:1400739/101‑1 (MQ=255) actactCGCATTGTGGA‑GGGGGGTGAAAGCCCGCGGCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCaa > 4:128570/1‑101 (MQ=255) actactAGAATTGTGGC‑GGGGGCTGAAAGCCCGCGGCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCaa > 4:47524/1‑101 (MQ=255) tactaGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAAc < 3:682710/101‑1 (MQ=255) actaGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACg < 3:958486/101‑1 (MQ=255) actaGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACg < 2:30413/101‑1 (MQ=255) aGAATTGTGGC‑GGGGGCTGAAAACCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCCAAGGATCGACGCCCAACGGTa > 3:436807/1‑101 (MQ=255) gtgtC‑GGGGGCTGAAAGCCGGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCAAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGAtt < 1:1373464/101‑1 (MQ=255) gggTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGAt > 1:569276/1‑101 (MQ=255) ggTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATc < 1:937351/101‑1 (MQ=255) | CAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGC‑GGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATC > NC_007779/2225607‑2225798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |