Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077791,512,9300TC42.5% 43.9 / 12.3 40L405P (CTC→CCC) ydcRfused DNA‑binding transcriptional regulator and amino transferase
Reads supporting (aligned to +/- strand):  ref base T (10/13);  new base C (0/17);  total (10/30)
Fisher's exact test for biased strand distribution p-value = 2.03e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTGCGCCGGGTAAAATGTTTTCTACCGGTGAAAACTGGTCA  >  NC_007779/1512830‑1513030
                                                                                                    |                                                                                                    
gCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCt                                                                                                      <  3:1159932/101‑1 (MQ=255)
gCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCt                                                                                                      >  3:742376/1‑101 (MQ=255)
gCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCt                                                                                                      <  3:728046/101‑1 (MQ=255)
gCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCt                                                                                                      >  3:368297/1‑101 (MQ=255)
  ggAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTcc                                                                                                    >  1:340393/1‑101 (MQ=255)
    aaCGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTcccc                                                                                                  <  3:1140128/101‑1 (MQ=255)
     aCGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCg                                                                                                 <  1:543994/101‑1 (MQ=255)
     aCGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCg                                                                                                 <  3:1200452/101‑1 (MQ=255)
            caacGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGtt                                                                                          >  1:1105920/1‑101 (MQ=255)
             accgggCCTGGCAGGCACTGCTGCGTTATCTGCGTGCGAAAGTGAAAATTCATCAAAATGACAGGGGTTTCTTCCTCTGGTGGGACCCCCCCGAGCCGTTa                                                                                         <  1:1125196/96‑1 (MQ=255)
                gTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGAt                                                                                      <  1:1379112/101‑1 (MQ=255)
                 tGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATg                                                                                     <  3:1477303/101‑1 (MQ=255)
                 tGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATg                                                                                     <  3:499201/101‑1 (MQ=255)
                   ccTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGcc                                                                                   <  1:1022917/101‑1 (MQ=255)
                    cTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCg                                                                                  >  3:30524/1‑101 (MQ=255)
                     tGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCgg                                                                                 <  1:147523/101‑1 (MQ=255)
                            caCTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTa                                                                          >  3:1189431/1‑101 (MQ=255)
                             aCTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTaa                                                                         >  3:541834/1‑101 (MQ=255)
                              ctgctgCGTTATCTGCCGGCGGAAGTGAAAATTCTTCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCGGAGCCGTTAGATGCCGGCGAATTAAg                                                                        <  1:997684/101‑1 (MQ=255)
                                 ctgcGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTCCTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCt                                                                     <  3:404002/101‑1 (MQ=255)
                                   gcGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCGGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTgg                                                                   <  3:1571076/101‑1 (MQ=255)
                                      ttATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggcgg                                                                <  1:1081520/101‑1 (MQ=255)
                                        aTCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCa                                                              >  3:379818/1‑101 (MQ=255)
                                          ctgcctgcGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACt                                                            <  1:3616/101‑1 (MQ=255)
                                             cctgcGGAAGTGAAAATTCATCATAATGACAGTGTTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGAc                                                         <  1:441828/101‑1 (MQ=255)
                                             cctgcGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGAc                                                         <  3:153792/101‑1 (MQ=255)
                                                gcGGAAGTGAAAATTCATCATAATGACGGTGGTTATTTTCTGTGGTTGGACCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGca                                                      <  3:719117/101‑1 (MQ=255)
                                                gcGGAAGTGAAAATTCATCATAATAAAAGTGGTTTCTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGca                                                      <  3:656060/101‑1 (MQ=255)
                                                       tGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATc                                               <  3:456721/101‑1 (MQ=255)
                                                           aaTTCATCATAAGGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTa                                           >  1:1041956/1‑101 (MQ=255)
                                                            aTTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTAt                                          <  1:550947/101‑1 (MQ=255)
                                                              tcatcaTAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTg                                        >  3:1137178/1‑101 (MQ=255)
                                                               catcatAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTgc                                       <  3:342789/101‑1 (MQ=255)
                                                               catcatAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTgc                                       <  1:152916/101‑1 (MQ=255)
                                                               catcatAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTgc                                       <  1:703009/101‑1 (MQ=255)
                                                                  catAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTGCGcc                                    <  3:1078768/101‑1 (MQ=255)
                                                                   atAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTGCGCCg                                   >  3:619518/1‑101 (MQ=255)
                                                                         aCAGTGTTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTGCGCCGGGTaaa                             <  1:734877/101‑1 (MQ=255)
                                                                          cAGTGTTTGTTTTCTCTGGTGGGAGCTCCCGGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTGCGCCGGGTaaaa                            <  3:313398/101‑1 (MQ=255)
                                                                           aGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTGCGCCGGGTAAAAt                           <  3:81226/101‑1 (MQ=255)
                                                                                     ttCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTGCGCCGGGTAAAATGTTTTCTAcc                 <  1:523014/101‑1 (MQ=255)
                                                                                        tctGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTGCGCCGGGTAAAATGTTTTCTACCGGt              <  3:1212514/101‑1 (MQ=255)
                                                                                          tGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTGCGCCGGGTAAAATGTTTTCTACCGGTGa            >  1:871421/1‑101 (MQ=255)
                                                                                               ggAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTGCGCCGGGTAAAATGTTTTCTACCGGTGAAAACt       <  3:116731/101‑1 (MQ=255)
                                                                                                    tCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTGCGCCGGGTAAAATGTTTTCTACCGGTGAAAACTGGTCa  >  1:211506/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGACGCATCATATCAGTATTGCGCCGGGTAAAATGTTTTCTACCGGTGAAAACTGGTCA  >  NC_007779/1512830‑1513030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑