New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	GFP_Plasmid_Sequ	3001 =	33048 (0.790)	15 (0.000) +AGTGTGACTCTAGTA	6/170	3.0	0.11%	intergenic (–/–)	–/–	–/–
	?	NC_007779	3464853 =	17 (0.370)					intergenic (‑229/+72)	murB/rrfB	UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding/5S ribosomal RNA
Rejected: Frequency below/above cutoff threshold.

GCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCG  >  NC_007779/3464785‑3464948                                                                     |                                                                                                ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccagtgtgactctagtaGAGAGCGTTCACCGACAAACAACAGATAAAACg                                                                 >  3:520740‑M2/69‑101 (MQ=255)        tgaacgctctctactagagtcacactggctcaccttcgggtgggccagtgtgactctagtaGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGcc                                                          >  3:634972‑M2/62‑101 (MQ=255)                    gTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGAc                                              <  3:648033/101‑1 (MQ=32)                    actagagtcacactggctcaccttcgggtgggccagtgtgactctagtaGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGAc                                              >  2:78235‑M2/50‑101 (MQ=255)                     ttGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACt                                             <  3:1121295/101‑1 (MQ=32)                       agagtcacactggctcaccttcgggtgggccagtgtgactctagtaGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGa                                           <  1:628856‑M2/55‑1 (MQ=255)                        gagtcacactggctcaccttcgggtgggccagtgtgactctagtaGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCGTTCTTTCTACTGAg                                          <  4:78130‑M2/56‑1 (MQ=255)                        cTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAg                                          >  3:418136/1‑101 (MQ=32)                         ttCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGc                                         >  3:1542648/1‑101 (MQ=32)                         ttCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGc                                         >  3:1132971/1‑101 (MQ=32)                           cGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCt                                       >  1:509395/1‑101 (MQ=32)                           cGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTCAGCCt                                       >  1:1567479/1‑101 (MQ=17)                            cacactggctcaccttcgggtgggccagtgtgactctagtaGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCtt                                      <  1:516052‑M2/60‑1 (MQ=255)                             cAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCttt                                     >  3:624817/1‑101 (MQ=32)                                cGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGt                                  >  1:1006644/1‑101 (MQ=32)                                     aaaTCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTAt                             <  1:164842/101‑1 (MQ=32)                                      cacccgccggtgcgcccgtgtgactctagtaGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTAtt                            >  4:141391‑M2/32‑101 (MQ=255)                                       acccgcgggtgcgcgagtgtgcctctagtaGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTAttt                           >  4:71863‑M2/31‑101 (MQ=255)                                       aTCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTAttt                           >  3:203149/1‑101 (MQ=32)                                          cGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGAt                        <  1:414808/101‑1 (MQ=33)                                           gCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATg                       >  1:587749/1‑101 (MQ=32)                                             tCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGcc                     >  1:1305702/1‑101 (MQ=32)                                              ggtgcgccagtgtgcctctcgtaGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCt                    >  4:130397‑M2/24‑101 (MQ=255)                                                ttaggcagtctgactctagtaTAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTgg                  <  1:1452600‑M2/80‑1 (MQ=255)                                                tgcgcccgtgtgactctagtcGCGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTgg                  >  4:53491‑M2/22‑101 (MQ=255)                                                tgatccagtgtgactctagtaGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTgg                  <  3:1605721‑M2/80‑1 (MQ=255)                                                tgatacagtgtgcctctagtaGAGAGCGTTCACCGACAAAAAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTgg                  <  1:979902‑M2/80‑1 (MQ=255)                                                 ggcggATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGc                 >  3:1504936/1‑101 (MQ=32)                                                    ggATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGggaa              <  3:303660‑M2/101‑5 (MQ=255)                                                    ccagtgtgactctagtaGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgcact              <  1:1319026‑M2/84‑3 (MQ=255)                                                     gATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGggaat             >  1:828983‑M2/1‑96 (MQ=255)                                                     gATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGggaat             >  1:434881‑M2/1‑96 (MQ=255)                                                     gATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGtt             >  1:469007/1‑101 (MQ=32)                                                     cagtgtgactctagtaGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTGCGTTGGAGGTGAGGCCTggcacta             <  4:99107‑M2/85‑4 (MQ=255)                                                         tGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGggaattggg         <  1:1473043‑M2/101‑10 (MQ=255)                                                         tGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGggaattggg         <  1:1078739‑M2/101‑10 (MQ=255)                                                          gTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGggaattaatt        >  3:1234588‑M2/1‑91 (MQ=255)                                                           tCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGggaattgggaa       >  1:782842‑M2/1‑90 (MQ=255)                                                                cTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCg  >  2:35158/1‑101 (MQ=32)                                                                     |                                                                                                GCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCG  >  NC_007779/3464785‑3464948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.