New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? GFP_Plasmid_Sequ 1622 =66540 (1.590)22 (0.000) 5/192 109.0 0.036% intergenic (–/–) –/– –/–
?GFP_Plasmid_Sequ 1734 = 59550 (1.480)intergenic (–/–) –/– –/–
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 62428 total aligned reads displayed.

TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGC  >  GFP_Plasmid_Sequ/1634‑1834
                                                                                                    |                                                                                                    
  aTGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTgcgc                                                                                                    <  3:1436179/101‑1 (MQ=255)
  aTGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTgcgc                                                                                                    <  3:1468801/101‑1 (MQ=255)
   tGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCa                                                                                                   >  1:1226538/1‑101 (MQ=255)
   tGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCa                                                                                                   >  1:394423/1‑101 (MQ=255)
   tGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCa                                                                                                   <  3:1371973/101‑1 (MQ=255)
      aaCTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTGGCAATGGCAACAACGTTGCGCAAAc                                                                                                <  3:1119750/101‑1 (MQ=255)
       aCTCGCCTTGATCGTTGGGACCCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACt                                                                                               <  1:694115/101‑1 (MQ=255)
       aCTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACt                                                                                               <  1:160761/101‑1 (MQ=255)
        cTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTa                                                                                              >  1:49610/1‑101 (MQ=255)
          cGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTAtt                                                                                            >  2:69717/1‑101 (MQ=255)
          cGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTAtt                                                                                            <  1:660461/101‑1 (MQ=255)
           gCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTa                                                                                           >  1:727017/1‑101 (MQ=255)
            ccTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTaa                                                                                          <  3:1536081/101‑1 (MQ=255)
            ccTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTaa                                                                                          <  1:1491993/101‑1 (MQ=255)
             cTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAAc                                                                                         >  4:48127/1‑101 (MQ=255)
             cTTGATCGTTGGAAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAAc                                                                                         >  3:1602275/1‑101 (MQ=255)
               tGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTg                                                                                       >  3:1213731/1‑101 (MQ=255)
               tGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTg                                                                                       <  3:573213/101‑1 (MQ=255)
                gATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTgg                                                                                      <  1:629715/101‑1 (MQ=255)
                gATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTgg                                                                                      >  1:1358217/1‑101 (MQ=255)
                 aTCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGc                                                                                     >  1:856727/1‑101 (MQ=255)
                 aTCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGc                                                                                     >  1:296329/1‑101 (MQ=255)
                 aTCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGc                                                                                     >  1:1098276/1‑101 (MQ=255)
                  tCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCg                                                                                    >  3:54272/1‑101 (MQ=255)
                  tCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCg                                                                                    <  3:1306416/101‑1 (MQ=255)
                  tCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCg                                                                                    <  1:1194605/101‑1 (MQ=255)
                    gTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGCCACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGaa                                                                                  >  1:563267/1‑101 (MQ=255)
                    gTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACGATTAACTGGCGaa                                                                                  <  4:73417/101‑1 (MQ=255)
                     ttGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAac                                                                                 <  3:923890/101‑1 (MQ=255)
                     ttGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAac                                                                                 <  3:893878/101‑1 (MQ=255)
                      tGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAact                                                                                <  3:1056591/101‑1 (MQ=255)
                      tGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAact                                                                                <  1:1390856/101‑1 (MQ=255)
                      tGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAact                                                                                <  3:957097/101‑1 (MQ=255)
                      tGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAact                                                                                <  3:702435/101‑1 (MQ=255)
                         gAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAactact                                                                             >  1:758686/1‑101 (MQ=255)
                           aCCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACtactta                                                                           >  3:1568812/1‑101 (MQ=255)
                            ccGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACtacttac                                                                          >  3:381328/1‑101 (MQ=255)
                              ggAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTAGCGAACTACTTActc                                                                        >  3:1501357/1‑101 (MQ=255)
                              gccgctgcctgacGCCATACCACCCGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTActc                                                                        >  4:115962/10‑101 (MQ=255)
                               gAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTActct                                                                       <  1:1458646/101‑1 (MQ=255)
                                     aatgaaGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTc                                                                 <  1:1557714/101‑1 (MQ=255)
                                       tgaaGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTccc                                                               <  1:891273/101‑1 (MQ=255)
                                       tgaaGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTccc                                                               >  3:477205/1‑101 (MQ=255)
                                           gCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGca                                                           >  3:1087883/1‑101 (MQ=255)
                                           gCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGca                                                           <  1:1033982/101‑1 (MQ=255)
                                             cATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACCCTAGCTTCCCGGcaac                                                         <  1:1525709/101‑1 (MQ=255)
                                              aTACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGcaaca                                                        <  1:428977/101‑1 (MQ=255)
                                               tACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGGAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGcaacaa                                                       >  1:330103/1‑101 (MQ=255)
                                               tACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGcaacaa                                                       <  3:67039/101‑1 (MQ=255)
                                                aCCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTCGCGAACTACTTACTCTAGCTTCCCGGCAACAAt                                                      >  3:797096/1‑101 (MQ=255)
                                                  cAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTa                                                    <  1:957767/101‑1 (MQ=255)
                                                   aaaCGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTaa                                                   <  1:1000032/101‑1 (MQ=255)
                                                   aaaCGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTaa                                                   >  2:116723/1‑101 (MQ=255)
                                                   aaaCGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTaa                                                   <  3:1245826/101‑1 (MQ=255)
                                                   aaaCGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTaa                                                   >  3:186423/1‑101 (MQ=255)
                                                     acgacgAGCGTGACACCACGATGTCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATa                                                 >  3:1404129/1‑101 (MQ=255)
                                                      cgacgaGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAg                                                >  1:1128404/1‑101 (MQ=255)
                                                      cgacgaGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAg                                                <  2:141924/101‑1 (MQ=255)
                                                      cgacgaGCGTGAAAAACCGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAg                                                >  4:22380/1‑101 (MQ=255)
                                                        acgaGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAc                                              <  3:98774/101‑1 (MQ=255)
                                                          gaGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACtg                                            >  1:792262/1‑101 (MQ=255)
                                                          gaGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACtg                                            >  3:283713/1‑101 (MQ=255)
                                                           aGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACtgg                                           >  1:1424995/1‑101 (MQ=255)
                                                           aGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACtgg                                           <  1:1067103/101‑1 (MQ=255)
                                                             cGTGACACCACGATGCCTGTAGCAATGGTAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACtggat                                         >  1:532479/1‑101 (MQ=255)
                                                             cGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACtggat                                         >  2:19799/1‑101 (MQ=255)
                                                                   accacGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGc                                   >  1:462436/1‑101 (MQ=255)
                                                                     cacGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCgg                                 >  2:45428/1‑101 (MQ=255)
                                                                      acGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGa                                >  3:1276502/1‑101 (MQ=255)
                                                                       cGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAt                               <  1:362039/101‑1 (MQ=255)
                                                                        gATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATa                              >  1:990391/1‑101 (MQ=255)
                                                                        gATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATa                              <  3:250860/101‑1 (MQ=255)
                                                                        gATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATa                              >  1:824703/1‑101 (MQ=255)
                                                                         aTGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATaa                             >  1:92618/1‑101 (MQ=255)
                                                                             cTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGtt                         <  3:412269/101‑1 (MQ=255)
                                                                               gTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGc                       >  3:733243/1‑101 (MQ=255)
                                                                                tAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCa                      <  3:317365/101‑1 (MQ=255)
                                                                                    aaTGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGAc                  >  1:1162428/1‑101 (MQ=255)
                                                                                    aaTGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGAc                  <  3:828620/101‑1 (MQ=255)
                                                                                     aTGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGAcc                 <  4:141649/101‑1 (MQ=255)
                                                                                      tGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCa                <  1:263178/101‑1 (MQ=255)
                                                                                      tGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCa                <  3:605168/101‑1 (MQ=255)
                                                                                         caacaaCGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACtt             <  1:1324849/101‑1 (MQ=255)
                                                                                          aacaacGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTc            >  1:195168/1‑101 (MQ=255)
                                                                                           acaacGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCt           <  2:94404/101‑1 (MQ=255)
                                                                                           acaacGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCt           <  3:1184645/101‑1 (MQ=255)
                                                                                             aacGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTgc         >  1:22856/1‑101 (MQ=255)
                                                                                              acGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTgcg        <  3:1338741/101‑1 (MQ=255)
                                                                                              acGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTGCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTgcg        <  3:1023479/101‑1 (MQ=255)
                                                                                               cGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTgcgc       >  3:1151288/1‑101 (MQ=255)
                                                                                               cGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTgcgc       <  3:508262/101‑1 (MQ=255)
                                                                                               cGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTgcgc       >  1:595881/1‑101 (MQ=255)
                                                                                                gTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCt      >  3:637964/1‑101 (MQ=255)
                                                                                                gTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCt      <  3:764589/101‑1 (MQ=255)
                                                                                                 ttGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTc     <  3:218755/101‑1 (MQ=255)
                                                                                                 ttGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTc     >  3:349216/1‑101 (MQ=255)
                                                                                                  tGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCg    <  1:1291719/101‑1 (MQ=255)
                                                                                                   gcgcAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCgg   <  1:1259198/101‑1 (MQ=255)
                                                                                                    cgcAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGc  <  3:445593/101‑1 (MQ=255)
                                                                                                    cgcAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGc  >  3:86016/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGC  >  GFP_Plasmid_Sequ/1634‑1834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.