Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pYTK001_with_tdk_kan	693	+G	intergenic (–/+1118)	– / ← CamR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pYTK001_with_tdk_kan	693	1	.	G	100.0%	147.8 / NA	38	intergenic (–/+1118)	–/CamR	–/–
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (25/13);  total (25/13)

AAGGTGAGCAGGTGGTAATTGGTGGTAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAA‑CGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATC  >  pYTK001_with_tdk_kan/572‑809                                                                                                                           |                                                                                                                     aaGGTGAGCAGGTGGTAATTGGTGGTAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTaagcgg                                                                                                                   <  2:358817/126‑5 (MQ=255)         cAGGTGGTAATTGGTGGTAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCAcc                                                                                                           >  1:113534/1‑126 (MQ=255)             ttgtAATTGGTGGTAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCgg                                                                                                       >  1:417580/3‑126 (MQ=255)              ggtAATTGGTGGTAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGt                                                                                                      >  1:278790/1‑126 (MQ=255)                 aaTGGGTGGTAATGAACGATACGTTTTTGTATGCCGTAAACACTATAAAGCGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGa                                                                                                   >  2:157916/1‑126 (MQ=255)                   ttGGTGGTAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGc                                                                                                 >  1:5559/1‑126 (MQ=255)                     ggtggtAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTc                                                                                               >  2:30884/1‑126 (MQ=255)                       tggtAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCgg                                                                                             >  1:176938/1‑126 (MQ=255)                            aTGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTAccc                                                                                        >  2:333825/1‑126 (MQ=255)                             tGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCg                                                                                       >  2:55766/1‑126 (MQ=255)                             tGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCg                                                                                       <  2:422705/126‑1 (MQ=255)                                      cGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGACATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGatcatc                                                                              <  1:320706/126‑1 (MQ=255)                                         ttCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTag                                                                           >  2:439862/1‑126 (MQ=255)                                            tGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGc                                                                        <  1:328258/126‑1 (MQ=255)                                             gTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCg                                                                       <  2:416975/126‑1 (MQ=255)                                               aTGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGa                                                                     >  2:381414/1‑126 (MQ=255)                                                  ccGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGAccc                                                                  >  2:373633/1‑126 (MQ=255)                                                      aaaCACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGACATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGa                                                              >  2:458699/1‑126 (MQ=255)                                                         cacTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAg                                                           <  1:334281/126‑1 (MQ=255)                                                         cacTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAg                                                           <  1:392924/126‑1 (MQ=255)                                                               aaaGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGt                                                     >  1:64826/1‑126 (MQ=255)                                                                  gagGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTtgt                                                  >  2:361966/1‑126 (MQ=255)                                                                      cGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGtct                                              <  2:5551/126‑1 (MQ=255)                                                                       gTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGtctc                                             >  1:109120/1‑126 (MQ=255)                                                                         tACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCaa                                           >  1:429966/1‑126 (MQ=255)                                                                            aaGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAAt                                        <  2:113390/126‑1 (MQ=255)                                                                            aaGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAAt                                        >  1:465693/1‑126 (MQ=255)                                                                               tCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAAtctc                                     >  1:200761/1‑126 (MQ=255)                                                                                     cATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGt                               <  2:176675/126‑1 (MQ=255)                                                                                            ggCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTg                        <  1:30924/126‑1 (MQ=255)                                                                                              cTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGttgcattgca                      >  2:270050/1‑126 (MQ=255)                                                                                              cTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGttgcattgca                      >  2:290907/1‑126 (MQ=255)                                                                                                aTTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGcaca                    >  2:408506/1‑126 (MQ=255)                                                                                                 ttCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACaa                   <  1:55833/126‑1 (MQ=255)                                                                                                 ttCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACaa                   <  2:108985/126‑1 (MQ=255)                                                                                                     ggAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATa               <  1:158607/126‑1 (MQ=255)                                                                                                      gAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATaa              >  2:199280/1‑126 (MQ=255)                                                                                                                  cACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATatcatc  >  1:419410/1‑126 (MQ=255)                                                                                                                  cACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATatcatc  >  1:187971/1‑126 (MQ=255)                                                                                                                           |                                                                                                                     AAGGTGAGCAGGTGGTAATTGGTGGTAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAA‑CGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATC  >  pYTK001_with_tdk_kan/572‑809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑