Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pYTK001_with_tdk_kan	782	A→G	intergenic (–/+1029)	– / ← CamR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pYTK001_with_tdk_kan	782	0	A	G	91.2%	105.8 / 2.1	34	intergenic (–/+1029)	–/CamR	–/–
Reads supporting (aligned to +/- strand):  ref base A (3/0);  new base G (11/20);  total (14/20)
Fisher's exact test for biased strand distribution p-value = 6.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GGCTATTCAGGAAAGGCATCGCCACGATTAA‑CGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAA  >  pYTK001_with_tdk_kan/663‑904                                                                                                                         |                                                                                                                           ggCTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTg                                                                                                                       <  1:30924/126‑1 (MQ=255)   cTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGttgcattgca                                                                                                                     >  2:290907/1‑126 (MQ=255)   cTATTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGttgcattgca                                                                                                                     >  2:270050/1‑126 (MQ=255)     aTTCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGcaca                                                                                                                   >  2:408506/1‑126 (MQ=255)      ttCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACaa                                                                                                                  <  1:55833/126‑1 (MQ=255)      ttCAGGAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACaa                                                                                                                  <  2:108985/126‑1 (MQ=255)          ggAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATa                                                                                                              <  1:158607/126‑1 (MQ=255)           gAAAGGCATCGCCACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATaa                                                                                                             >  2:199280/1‑126 (MQ=255)                       cACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATatcatc                                                                                                 >  1:187971/1‑126 (MQ=255)                       cACGATTAAGCGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATatcatc                                                                                                 >  1:419410/1‑126 (MQ=255)                            ttaagcGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGaa                                                                                            <  1:485315/121‑1 (MQ=255)                                 cGGCCTCCACCGCGGTGCAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATa                                                                                       >  2:345724/1‑126 (MQ=255)                                 cGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATa                                                                                       <  1:291343/126‑1 (MQ=255)                                 cGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATa                                                                                       <  1:270459/126‑1 (MQ=255)                                   gccgccACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATaaa                                                                                     <  1:374160/126‑1 (MQ=255)                                          ccGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCt                                                                              <  1:396370/126‑1 (MQ=255)                                              ggTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCtt                                                                          >  1:226957/1‑126 (MQ=255)                                                gggggcccGGTACCCGGGGATCCTCTTGAGCGGACCCGGGAAAGCCACGTTGTTTCTCAAAATCTCTTATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTAc                                                                        <  2:200455/119‑1 (MQ=255)                                                    gCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATaa                                                                    <  1:158145/126‑1 (MQ=255)                                                    gCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATaa                                                                    <  1:440491/126‑1 (MQ=255)                                                       cGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACa                                                                 <  1:59506/126‑1 (MQ=255)                                                            cccGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAAt                                                            <  1:409099/126‑1 (MQ=255)                                                               ggggATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACa                                                         <  2:64746/126‑1 (MQ=255)                                                                gggATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACaa                                                        <  1:381955/126‑1 (MQ=255)                                                                     ccTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGgt                                                   >  2:173399/1‑126 (MQ=255)                                                                      ctctAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGgtg                                                  >  1:117953/1‑126 (MQ=255)                                                                      ctctAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGgtg                                                  >  1:496133/1‑126 (MQ=255)                                                                          agagCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTAt                                              <  2:278373/126‑1 (MQ=255)                                                                           gagCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATg                                             >  1:360317/1‑126 (MQ=255)                                                                                                tttGTGTCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACg                        <  2:429351/125‑1 (MQ=255)                                                                                                    tgtCTCAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCtt                    >  1:498750/1‑126 (MQ=255)                                                                                                        tcAAAATCTCTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTc                <  1:362474/126‑1 (MQ=255)                                                                                                              tctcTGATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGcc          <  1:459361/126‑1 (MQ=255)                                                                                                                   gATGTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGAt     >  1:226414/1‑126 (MQ=255)                                                                                                                      gTTGCATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTaa  >  2:146747/1‑126 (MQ=255)                                                                                                                      gTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTaa  >  2:311347/1‑126 (MQ=255)                                                                                                                         |                                                                                                                           GGCTATTCAGGAAAGGCATCGCCACGATTAA‑CGGCCGCCACCGCGGTGGAGCTCGGTACCCGGGGATCCTCTAGAGCGGACCCGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAA  >  pYTK001_with_tdk_kan/663‑904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑