---+++ YPD Medium General media used for culturing Yeast. Adapted from [[http://cshprotocols.cshlp.org/content/2010/9/pdb.rec12315.full][Cold spring harbor]] In 1 L bottle: | *1L* | *Component* | | 20g | Peptone | | 10 g | Yeast Extract | * Dissolve above in ~900 mL water. * Bring to 950 mL (final volume will be 1L after autoclaving) In separate 500mL bottle, prepare 40% Glucose w/v Autoclave separately. *WARNING* If glucose added before autoclaving, media will caramelize and be unusable. * Add 50 mL of 40% glucose solution to YPD media.
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Topic revision: r3 - 2022-06-01 - JeffreyBarrick