---+++ TA: Tetrazolium Sugar (TA, TM, TL, ...) Combine in a 2 L flask: | *1.5L* | *Component* | | 15 g | Tryptone | | 1.5 g | Yeast Extract | | 7.5 g | NaCl | | 24 g | Agar | | 1.5 ml | 5% Antifoam | Add dH<sub>2</sub>O to 1.3 liter. Combine in a 500 ml flask: | *1.5L* | *Component* | | 15 g | Sugar (arabinose = TA, maltose = TM, lactose = TL) | Add dH<sub>2</sub>O to 0.2 liter. Autoclave sugar and media separately. Total combined volume is 1.5 liters. Combine and add: | *1.5L* | *Component* | | 1.5 ml | 5% (w/v) Tetrazolium chloride (TTC), filter sterilized, stored at 4°C | Source: [[http://myxo.css.msu.edu/ecoli/taagar.html][Lenski Lab Protocol]]
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Topic revision: r6 - 2018-10-25 - JeffreyBarrick