TA: Tetrazolium Sugar (TA, TM, TL, ...)
Combine in a 1 L flask:
| 500 mL |
Component |
| 5 g |
Tryptone |
| 500 mg |
Yeast Extract |
| 2.5 g |
NaCl |
| 8 g |
Agar |
| 500 µL |
5% Antifoam |
Add dH
2O to 433.33 mL.
Combine in a 125 ml flask:
| 500 mL |
Component |
| 5 g |
Sugar (arabinose = TA, maltose = TM, lactose = TL) |
Add dH
2O to 66.67 mL.
Cover each flask with foil, and autoclave sugar and media separately.
Total combined volume will be 500 mL.
Combine the autoclaved sugar and media solutions and add:
| 500 mL |
Component |
| 500 µL |
5% (w/v) Triphenyl Tetrazolium chloride (TTC), filter sterilized, stored at 4°C |
Source:
Lenski Lab Protocol