---+++ TA: Tetrazolium Sugar (TA, TM, TL, ...) Combine in a 2 L flask: | 15 g | Tryptone | | 1.5 g | Yeast Extract | | 7.5 g | NaCl | | 24 g | Agar | | 1.5 ml | 5% Antifoam | or | .75 ml | 10% Antifoam | Add dH<sub>2</sub>O to 1.3 liter. Combine in a 500 ml flask: | 6 g | Sugar (arabinose = TA, maltose = TM, lactose = TL) | Add dH<sub>2</sub>O to 0.2 liter. Autoclave sugar and media separately. Total combined volume is 1.5 liters. Combine and add: | 1.5 ml | TTC (5%) | or | 7.5 ml | TTC (1%) |
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Topic revision: r1 - 2011-06-14 - CraigBarnhart